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| A shared resource available for researchers with appointments in the Vanderbilt University Medical Center, supported by the Vanderbilt-Ingram Cancer Center, the Diabetes Research and Training Center, the Digestive Disease Research Center, the Kennedy Center, the Vision Research Center, and the Mouse Metabolic Phenotyping Center. |
Zeiss Axiophot Microscope |
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Why Use This Microscope?This microscope is well suited for viewing samples upright on slides using a SEALED #1.5 coverglass. This instrument is not to be used with dishes or open containers of any sort - these formats should be used on the Leica inverted microscope. All slides MUST be sealed, clean and dry prior to placing on the stage. |
Location:
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Imaging Modes:
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Detector/Cameras: Princeton Instruments MicroMax CCD camera for low-light, quantitative fluorescence Photometrics CoolSnap digital camera for color brightfield images, can also be used for multi-color fluorescence, but less sensitive than the MicroMax and not quantitative. Explanation of size of digital images taken by CCD cameras Two 35mm film cameras with Zeiss computerized controller |
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Fluorescence filters: (excitation/emission) UV/Blue (365 nm for blue dyes such as DAPI and Hoechst) |
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Objective lenses: 5x / 0.15 Plan-NEOFLUAR |
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Charges: $35 Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Carol Ann, Bob, Mary or Sam, to arrange training. Download/View Quickstart Guide. |
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