Why Use This Microscope?

This microscope is well suited for viewing polarized cells and tissue samples on sealed, inverted slides or in 35mm Matek dishes with a #1.5 coverglass. Imaging through a non-glass surface is discouraged. All slides MUST be sealed, clean and dry prior to placing on the stage.

Imaging Modes:

Laser scanning fluorescence and DIC (Nomarski), 3-D "Z-series," and time-series. Simultaneous bleach/scan for PA-GFP and FRAP.

Fluorescence filters: (excitation/emission)

NearUV/Blue (405nm for UV dyes e.g, DAPI)
Blue/Green (457,488,515nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
Green/Red (543nm for Cy-3, Alexa568 or rhodamine-like dyes)
Red/Far-Red (633nm for far-red dyes like Alexa 633, Cy-5)

Objective lenses:

10x / 0.30 Plan Neofluar
20x / 0.80 SPlan-UApo
40x / 1.30 Plan-Neofluar OIL
60x / 1.45 Plan-Apochromat OIL
100x / 1.40 SPlan-UApo OIL

Charges: $35 per hour

Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Carol Ann, Bob, or Sean to arrange training.

Download/View Quickstart Guide.

VUMC Home About VUMC | Health Care Services | Schools | Research | Search ]

Copyright © 1998-2008, Vanderbilt University Medical Center
URL: http://www.mc.Vanderbilt.Edu/cellimage/index.htm
For More Information: <sam.wells@vanderbilt.edu>
Last Modified: Wednesday, August 13, 2008
<sean.schaffer@vanderbilt.edu>