Why Use This Microscope?

This microscope is well suited for viewing polarized cells and tissue samples on sealed, inverted slides or in 35mm Matek dishes with a #1.5 coverglass. All slides MUST be sealed, clean and dry prior to placing on the stage.

Imaging Modes:

Laser scanning fluorescence and DIC (Nomarski), Spectral Imaging, 3-D "Z-series", and time-series.

Fluorescence filters: (excitation/emission)

Blue/Green (457,488,514nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
Red/Far-Red (633 nm for far-red dyes like Cy-5)

Objective lenses:

5x/.30 Plan-Neofluar
10x / 0.30 Plan Neofluar
20x / 0.75 Plan-Apochromat
40x / 1.30 Plan-Neofluar OIL
63x / 1.40 Plan-Apochromat OIL
100x / 1.30 Plan-Neofluar OIL

Charges: $35 per hour

Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Sean, Carol Ann, Bob, Mary or Sam, to arrange training.

Download/View Quickstart Guide.

VUMC Home About VUMC | Health Care Services | Schools | Research | Search ]

Copyright © 1998-2008, Vanderbilt University Medical Center
URL: http://www.mc.Vanderbilt.Edu/cellimage/index.htm
For More Information: <sam.wells@vanderbilt.edu>
Last Modified: Tuesday, February 5, 2008
<sean.schaffer@vanderbilt.edu>