A shared resource available for researchers with appointments in the Vanderbilt University Medical Center, supported by the Vanderbilt-Ingram Cancer Center, the Diabetes Research and Training Center, the Digestive Disease Research Center, the Kennedy Center, the Vision Research Center, and the Mouse Metabolic Phenotyping Center.
 

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Zeiss Inverted LSM510 Confocal Microscope

Why Use This Microscope?

This microscope is well suited for viewing samples on sealed, inverted slides or in 35mm dishes with a #1.5 coverglass (Matek) compatible with the inverted format. All slides MUST be sealed, clean and dry prior to placing on the stage

Location:

742 Light Hall

Imaging Modes:

Laser scanning fluorescence and DIC (Nomarski), 3-D "Z-series," and time-series.

Fluorescence filters: (excitation/emission)

    Blue/Green (457,488,514nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
    Green/    Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
    Red/    Far-Red (633 nm for far-red dyes like Cy-5)

Objective lenses:

    10x / 0.30 Plan Neofluar
    20x / 0.75 Plan-Apochromat
    25x / 0.80 Multi-Immursion
    40x / 1.30 Plan-NEOFLUAR OIL
    63x / 1.40 Plan-APOCHROMAT OIL
    100x / 1.30 Plan-NEOFLUAR OIL

Charges: $35 per hour

Note: To use the microscope you must have an active Account with the Cell Imaging Shared Resource and reserve time using our on-line Scheduling calendar. Contact Dawn, Carol Ann, Sean, Shawn or Sam, to arrange training.

Download/View Quickstart Guide.

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URL: http://www.mc.Vanderbilt.Edu/cellimage/index.htm
For More Information: <sam.wells@vanderbilt.edu>
Last Modified: Tuesday, February 5, 2008
<sean.schaffer@vanderbilt.edu>