Microscopy Mini-Course, May 14-18, 2007

(Sponsored by the Cell Imaging Shared Resource and various corporate contributions – see below)

 

Please refer to this site immediately prior to the course for last minute information, including room numbers and protocols.  The lectures are important and will be in Room 1220 MRBIII, except Tues (5/15) in Stevenson 4327.  The lectures are not restricted – anyone can attend.

 

The labs are restricted.  Click here to view Lab Assignments.  Lab locations and other instructions will be provided at the beginning of morning lectures.  The labs are for exploring basic concepts learned in the lectures.  If you are assigned to a lab, you must attend the relevant lecture or forfeit your lab assignment.

 

Monday, May 14                   Introduction to Optical Microscopy

09:00 – 09:15                       Introduction and Overview of Mini-course

09:15 – 10:30                       Transmitted Light Microscopy: definitions, principles, contrast modes (Piston)

10:30 – 10:45                       Break

10:45 – 12:00                       Fluorescence Microscopy: overview of fluorescence, definitions, principles (Wells)

 

    1:00 – 2:30                       Lab 1: identify knobs, buttons and hardware, practice alignments, Kφhler, BF, Ph, DIC (staff)

    2:30 – 3:00                       Break

    3:00 – 4:30                       Lab 2: fluorescence –practice alignments, select filters, control exposure and contrast (staff)

 

Tuesday, May 15                  Digital Wide-field Microscopy  (lectures in Stevenson 4327)

09:15 – 10:30                       Solid-state Detectors and Computers: compared with eye and brain or film (Piston)

10:30 – 10:45                       Break

10:45 – 12:00                       Quantitative Microscopy: assigning meaningful numbers to microscope images (Wells)

 

    1:00 – 2:30                       Lab 3(a): acquire digital images – reducing noise (staff)

    2:30 – 3:00                       Break

    3:00 – 4:30                       Lab 4(a): manipulate digital images – dynamic range and threshold (staff)

 

Wednesday, May 16            Live Cell Imaging

09:15 – 10:30                       Principles of Live Cell Imaging (Rocheleau)

10:30 – 10:45                       Break

10:45 – 12:00                       Advanced Imaging Techniques: FRET, FRAP, FLIP, FLIM, TIRF, etc. (Goodwin)

 

    1:00 – 2:30                       Lab 5a(c): live cell time-lapse/special imaging-1 (staff)

    2:30 – 3:00                       Break

    3:00 – 4:30                       Lab 5b(c): live cell time-lapse/special imaging-2 (staff)

 

Thursday, May 17               Confocal Microscopy and 3D-Resolved Imaging

09:15 – 10:30                       Basic Principles and Advanced Capabilities of Confocal Microscopy (Wells)

10:30 – 10:45                       Break

10:45 – 12:00                       Other 3D Techniques: Spinning Disk, Deconvolution, 2PEF (Rocheleau)

 

   1:00 – 04:30                      open for vendor equipment demonstrations

 

Friday, May 18                      Electron Microscopy

09:00 – 09:15                       Course Summary

09:15 – 10:30                       Overview of TEM and SEM, EM Specimen Preparation (Jerome)

10:30 – 10:45                       Break

10:45 – 12:00                       Schemes for Correlating Data from Light and Electron Microscopy (Jerome)

 

    1:00 – 2:30                       Lab 6(a): dimensions, lens NA, pinholes, resolution (staff)

    2:30 – 3:00                       Break

    3:00 – 4:30                       Lab 7(b): FRET imaging (staff)

                             

 

Prerequisites

a)  Requires skills developed in Labs 1, 2 or elsewhere.

b)  Requires skills developed in Labs 1, 2 and 5 or elsewhere.

c)  Requires skills developed in Labs 1, 2 and 3 or elsewhere.

 

 

Corporate Contributions of Equipment, Material and Staff by:

Nikon, Olympus America, Roper Scientific, Carl Zeiss, Invitrogen (Molecular Probes)