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| A shared resource available for researchers with appointments in the Vanderbilt University Medical Center, supported by the Vanderbilt-Ingram Cancer Center, the Diabetes Research and Training Center, the Digestive Disease Research Center, the Kennedy Center, the Vision Research Center, and the Mouse Metabolic Phenotyping Center. |
What size are my images? The following values apply to the Axiophot microscope front and back camera ports. Note that both cameras clip the edges of the field, so that you take a picture of only the center of the view through the oculars. |
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Objective
lens used |
CoolSnap
rear port |
MicroMax
front port |
| 2.5x | 2040x1520 | 3420x2640 |
| 5x | 1020x760 | 1800x1410 |
| 10x | 510x380 | 900x705 |
| 20x | 255x190 | 450x353 |
| 40x | 128x95 | 225x176 |
| 63x | 81x60 | 140x110 |
| 100x | 51x38 | 90x70 |
| Resolution (pixels) | 1392x1040 | 1317x1035 |
The "magnification" factor of an image is dependent on the size that you ultimately display or print the image. When looking through the microscope while using a 20X objective lens, with the standard 10X ocular lenses, what you see is a 200X magnified image. But consider this example: If you take an image with the 20X objective lens using the MicroMax CCD, your field size is 450 micrometers. If this is printed, as is common, to take up one column in a journal, it will be about 55mm wide, which is roughly 122X magnification. But if the same image is printed on the cover, taking up the full page, it will be about 200mm, or almost 444X. For this reason, it is often preferable to simply insert a scale bar in the image, the length of which is determined by its size as a fraction of the whole image, which is known. |
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