Phenotype Core | Molecular Genetics Core | Immunohistochemistry Core | Home
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Available Core ServicesSDRCC Phenotype Core For Animal Surgical Services |
| Standard Rat Models: | Includes up to four dorsal 6mm full thickness excisions or incisions, one ventral incision for placement of 4 PVA sponges (dead space wounds). Average study duration: 7 to 14 days. |
Standard Rat Model plus diabetic induction. Modification: stent / ischemia: |
Includes the above plus diabetic induction with streptozotocin and monitoring. |
| Typical Methods of Analysis: | Wound closure study of excisions (digital planimetry) |
Doppler perfusion (Lisca) study of excisions (superficial blood flow) |
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Histological and Morphometric Analysis of sponges (epithelialization, granulation, tissue area, vascularity, inflammation) |
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BTC or Instron analysis of incision (mechanical properties) |
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HPLC analysis for Collagen and Elastin Content (based on Hydroxyproline and Desmosine determination |
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| Standard Mouse Model: | Includes two dorsal, 6mm full thickness excisions, one ventral incision for placement of 2-4 PVA sponges (dead space wounds). Average study duration: 7 to 14 days |
| Standard Mouse Model plus diabetic induction. Modification: stent / ischemia: | Includes the above plus diabetic induction and monitoring. |
| Typical Methods of Analysis: | Wound closure study of excisions |
Doppler perfusion (Lisca) study of excisions |
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Histological and Morphometric Analysis of sponges |
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BTC or Instron analysis of incision |
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HPLC analysis for hydroxyproline |
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DNA and total protein determination |
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| Rabbit Ear Excision Model: | Includes four full thickness excisions (down to the cartilage) on the interior aspect of ear. Average study duration: 7-28 days |
| Parameters: | Wound closure, histology, morphometry, laser doppler perfusion imaging, biomechanical analysis |
Analysis Tools:
For in situ testing of wound closure strength in a destructive or nondestructive format.
Typically used in rodents with incisional wounds not more that 10 days old.
For in situ testing of blood perfusion around and in healing wounds (excisional or dead space). Data to be collected over course of a study.
For testing strips of skin 1 cm wide and 3-5 cm long that includes the area of interest (healed wound or normal skin). Data is a terminal measure of breaking strength.
Includes digital photograph of excisional wounds over the course of the study. Makes use of analytical software to measure wound closure over time.
In cooperation with the SDRC Morphology Core the Phenotype Core will provide analysis of results based upon H/E, trichrome and other available staining regimes. (Morphology core and Phenotype core charges apply)
In cooperation with the SDRC Morphology Core the Phenotype Core will provide analysis of results based upon reepithelialization, quantification of other markers of wound healing (presence of collagen, granulation tissue, inflammation, angiogenesis.)
(Morphology core and Phenotype core charges apply)
For determining percentage of collagen in harvested wounds. Total collagen and other amino acid determinations available as well as elastin.
Colorimetric determination of protein in samples. Typically done in conjunction with HPLC.
Colorimetric determination of DNA in samples. Typically done in conjunction with HPLC.
Includes preparation and adminstration of gene bearing plasmids into wound sites or normal skin with the use of the Helios (BIORAD) Gene Gun.
Includes the above plus diabetic induction and monitoring.
Custom Services available upon request. Our surgical expertise and technological capability are adaptable to your application.
* indicates not a “facilities only” service—“full service” charges apply
For additional information or scheduling contact:
Core Director:
Jeffrey M. Davidson, Ph.D.
Phone: (615) 327-4751 ext. 5488
Fax: (615) 327-5393
E-Mail: jeff.davidson@vanderbilt.edu
Location:
Small Animal Model: Cher Carlisle (3-2321) U-2215 MCN