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Available Core Services

SDRCC Phenotype Core For Animal Surgical Services

Standard Rat Models:

Includes up to four dorsal 6mm full thickness excisions or incisions, one ventral incision for placement of 4 PVA sponges (dead space wounds). Average study duration: 7 to 14 days.

Standard Rat Model plus diabetic induction. Modification: stent / ischemia:

Includes the above plus diabetic induction with streptozotocin and monitoring.

Typical Methods of Analysis:

Wound closure study of excisions (digital planimetry)

 

Doppler perfusion (Lisca) study of excisions (superficial blood flow)

 

Histological and Morphometric Analysis of sponges (epithelialization, granulation, tissue area, vascularity, inflammation)

 

BTC or Instron analysis of incision (mechanical properties)

 

HPLC analysis for Collagen and Elastin Content (based on Hydroxyproline and Desmosine determination

   
Standard Mouse Model:

Includes two dorsal, 6mm full thickness excisions, one ventral incision for placement of 2-4 PVA sponges (dead space wounds). Average study duration: 7 to 14 days

Standard Mouse Model plus diabetic induction. Modification: stent / ischemia:

Includes the above plus diabetic induction and monitoring.

Typical Methods of Analysis:

Wound closure study of excisions

 

Doppler perfusion (Lisca) study of excisions

 

Histological and Morphometric Analysis of sponges

 

BTC or Instron analysis of incision

 

HPLC analysis for hydroxyproline

 

DNA and total protein determination

Rabbit Ear Excision Model:

Includes four full thickness excisions (down to the cartilage) on the interior aspect of ear.  Average study duration: 7-28 days

Parameters:

Wound closure, histology, morphometry, laser doppler perfusion imaging, biomechanical analysis

   

Analysis Tools:

Biomechanical Tissue Characterization (BTC):

For in situ testing of wound closure strength in a destructive or nondestructive format.
Typically used in rodents with incisional wounds not more that 10 days old.

Doppler Perfusion Imaging (Lisca):

For in situ testing of blood perfusion around and in healing wounds (excisional or dead space).  Data to be collected over course of a study.

Analysis of Tensile Strength (Instron):

For testing strips of skin 1 cm wide and 3-5 cm long that includes the area of interest (healed wound or normal skin).  Data is a terminal measure of breaking strength.

Wound Closure Analysis:

Includes digital photograph of excisional wounds over the course of the study.  Makes use of analytical software to measure wound closure over time.

Histological Analysis: *

In cooperation with the SDRC Morphology Core the Phenotype Core will provide analysis of results based upon H/E, trichrome and other available staining regimes.  (Morphology core and Phenotype core charges apply)

Morphometric Analysis: *

In cooperation with the SDRC Morphology Core the Phenotype Core will provide analysis of results based upon reepithelialization, quantification of other markers of wound healing (presence of collagen, granulation tissue, inflammation, angiogenesis.)
(Morphology core and Phenotype core charges apply)

HPLC Analysis for Hydroxyproline and Desmosine / Isodesmosine: *

For determining percentage of collagen in harvested wounds.  Total collagen and other amino acid determinations available as well as elastin.

Protein Quantification: *

Colorimetric determination of protein in samples.  Typically done in conjunction with HPLC.

DNA Quantification: *

Colorimetric determination of DNA in samples. Typically done in conjunction with HPLC.

Gene Gun:

Includes preparation and adminstration of gene bearing plasmids into wound sites or normal skin with the use of the Helios (BIORAD) Gene Gun.

Gene Gun plus diabetic induction:

Includes the above plus diabetic induction and monitoring.

Custom Services:

Custom Services available upon request.  Our surgical expertise and technological capability are adaptable to your application.

* indicates not a “facilities only” service—“full service” charges apply

For additional information or scheduling contact:

Core Director:
Jeffrey M. Davidson, Ph.D.
Phone: (615) 327-4751 ext. 5488
Fax: (615) 327-5393
E-Mail: jeff.davidson@vanderbilt.edu

Location:
Small Animal Model: Cher Carlisle (3-2321) U-2215 MCN