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SDRCC - Phenotype Core:

Post-Surgical Analysis Tools

Vascular Perfusion Analysis

The Laser Doppler Perfusion Imager scans the skin surface and identifies areas of increased blood flow. This tool is helpful in determining the intensity of response in angiogenesis enhancing test materials as well as inflammation erythema. Penetration is 2mm or less.

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Tensiometry

Tensiometry is used to determine the ultimate breaking strength and other biomechanical parameters of a tissue sample. A tissue sample is placed between two clamping devices and is slowly pulled apart until the area under test is separated. Highly sensitive strain gauges record the real time patterns of failure for graphic analysis. Results are calculated as breaking strength, breaking energy, elastic modulus, and tensile strength.

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Bio-Mechanical Tissue Analysis

This device allows in situ testing of the integrity of an area of skin by using a high vacuum coupled with a laser deflection detector to determine skin elasticity, breaking pressure, laxity, deformation, etc. Two different methods are used to gather this data: a non-destructive method for determining the deformability of skin; a destructive (rupture) method, done post-mortem to determine the detailed tensile properties of incisional wounds.

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Collagen and Elastin Analysis
 
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Gene Gun

The Bio-Rad, hand-held Helios Gene Gun makes possible the transfection and expression of DNA in living tissue by utilizing a biolistic method of propelling tiny gold particles carrying plasmid DNA into the target tissues. This method creates dispersed and superficial implantation of plasmids while minimizing tissue damage.

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