Center for Matrix Biology - Vanderbilt University and Medical Center

Billy G. Hudson, Ph.D.
Professor in the Department of Medicine
Elliot V. Newman Professor of Medicine, Pathology and Biochemistry
Director, Vanderbilt Center for Matrix Biology

	Office Address:	B-3102 Medical Center North
	Mailing Address:	1161 Twenty-first Avenue South B-3102 Medical Center North Nashville, TN 37232-2372
	Lab address:	B-3102 Medical Center North
	Phone:	615-322-7298
		email \| website

Secondary appointment in the Pathology department.
Secondary appointment in the Biochemistry department.

Research Summary

My research program focuses on the structure and function of type IV collagen, the major constitutent of basement membranes (BMs). BMs are a specialized form of extracellular matrix that compartmentalizes tissues and play an important role in tissue remodeling and morphogenesis. BM function is impaired in both hereditary and acquired diseases that affect type IV collagen. In Alport syndrome, a hereditary renal disease, the collagen genes are mutated. In Goodpasture syndrome, a rapidly progressive renal disease with lung hemorrhage, the collagen is the target for autoantibodies. In diabetes, the vascular complications of nephropathy and retinopathy also appear to involve this collagen.
Type IV collagen has recently emerged as a family of six homologous alpha-chains, designated alpha1 to alpha6. My research group discovered the alpha3 and alpha4 chains. The alpha3 chain is the target for autoantibodies in Goodpasture syndrome and the alpha5 chain is the target for autoantibodies in a novel autoimmune disease that causes renal disease and skin blistering. The alpha3, alpha4, and alpha5 chains are mutated in Alport syndrome causing defective assembly of a supramolecular network which leads to progressive renal failure. Recently we have shown that the code for the assembly of chain-specific networks resides within the noncollagenous (NC1) domain of the collagen chains. The three-dimensional structure of this domain was recently determined by x-ray crystallography, revealing novel information about structure and function.
Most recently, we discovered that type IV collagen may represent an important new class of anti-angiogenic molecules for the treatment of neovascular diseases, such as tumor growth and metastasis. Our findings have led to the development of two drugs: Pyridorin, a molecule inhibitor for the treatment of diabetic renal disease now in phase II trials, and Angiocol an anti-angiogenesis agent that targets basal lamina, slated for a phase I trial next year.

Research Keywords

Biochemistry, Cancer, Developmental biology, Diabetes, Immunology, Kidney, Malignancy, Pathology, Protein Structure, Renal biology, Structural Biology, Vascular Biology,

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Associated initiative(s):

Angiogenesis

Diabetic Complications

Fibrosis

Tumor Microenvironment

Career opportunities:
None currently available

Selected Publications

» Rebustini, IT, Myers, C, Lassiter, KS, Surmak, A, Szabova, L, Holmbeck, K, Pedchenko, V, Hudson, BG, Hoffman, MP MT2-MMP-Dependent Release of Collagen IV NC1 Domains Regulates Submandibular Gland Branching Morphogenesis Dev Cell 2009. 17:482-93

» Aggeli, AS, Kitsiou, PV, Tzinia, AK, Boutaud, A, Hudson, BG, Tsilibary, EC Selective binding of integrins from different renal cell types to the NC1 domain of alpha3 and alpha1 chains of type IV collagen J Nephrol 2009. 22:130-6

» Vanacore, R, Ham, AJ, Voehler, M, Sanders, CR, Conrads, TP, Veenstra, TD, Sharpless, KB, Dawson, PE, Hudson, BG A sulfilimine bond identified in collagen IV Science 2009. 325:1230-4

» Abrahamson, DR, Hudson, BG, Stroganova, L, Borza, DB, St John, PL Cellular origins of type IV collagen networks in developing glomeruli J Am Soc Nephrol 2009. 20:1471-9

» Pozzi, A, Voziyan, PA, Hudson, BG, Zent, R Regulation of matrix synthesis, remodeling and accumulation in glomerulosclerosis Curr Pharm Des 2009. 15:1318-33

» Pozzi, A, Jarad, G, Moeckel, GW, Coffa, S, Zhang, X, Gewin, L, Eremina, V, Hudson, BG, Borza, DB, Harris, RC, Holzman, LB, Phillips, CL, Fassler, R, Quaggin, SE, Miner, JH, Zent, R Beta1 integrin expression by podocytes is required to maintain glomerular structural integrity Dev Biol 2008. 316:288-301

» Kang, JS, Colon, S, Hellmark, T, Sado, Y, Hudson, BG, Borza, DB Identification of noncollagenous sites encoding specific interactions and quaternary assembly of alpha 3alpha 4alpha 5(IV) collagen: Implications for Alport gene therapy J Biol Chem 2008. :

» Borza, CM, Borza, DB, Pedchenko, V, Saleem, MA, Mathieson, PW, Sado, Y, Hudson, HM, Pozzi, A, Saus, J, Abrahamson, DR, Zent, R, Hudson, BG Human podocytes adhere to the KRGDS motif of the alpha3alpha4alpha5 collagen IV network J Am Soc Nephrol 2008. 19:677-84

» Calvete, JJ, Revert, F, Blanco, M, Cervera, J, T?!rrega, C, Sanz, L, Revert-Ros, F, Granero, F, P??rez-Pay?!, E, Hudson, BG, Saus, J Conformational diversity of the Goodpasture antigen, the noncollagenous-1 domain of the alpha3 chain of collagen IV Proteomics 2008. 8:217

» Khoshnoodi, J, Pedchenko, V, Hudson, BG Mammalian collagen IV Microsc Res Tech 2008. :

» Granero-Molt??, F, Sarmah, S, O''Rear, L, Spagnoli, A, Abrahamson, D, Saus, J, Hudson, BG, Knapik, EW Goodpasture antigen-binding protein and its spliced variant, ceramide transfer protein, have different functions in the modulation of apoptosis during zebrafish development J Biol Chem 2008. :

» Chetyrkin, SV, Mathis, ME, Ham, AJ, Hachey, DL, Hudson, BG, Voziyan, PA Propagation of protein glycation damage involves modification of tryptophan residues via reactive oxygen species: inhibition by pyridoxamine Free Radic Biol Med 2008. 44:1276-85

» Abair, TD, Sundaramoorthy, M, Chen, D, Heino, J, Ivaska, J, Hudson, BG, Sanders, CR, Pozzi, A, Zent, R Cross-talk between integrins alpha1beta1 and alpha2beta1 in renal epithelial cells Exp Cell Res 2008. :

» Vanacore, RM, Ham, AJ, Cartailler, JP, Sundaramoorthy, M, Todd, P, Pedchenko, V, Sado, Y, Borza, DB, Hudson, BG A Role for Collagen IV Cross-links in Conferring Immune Privilege to the Goodpasture Autoantigen: STRUCTURAL BASIS FOR THE CRYPTICITY OF B CELL EPITOPES J Biol Chem 2008. 283:22737-22748

» Khoshnoodi, J, Hill, S, Tryggvason, K, Hudson, B, Friedman, DB Identification of N-linked glycosylation sites in human nephrin using mass spectrometry J Mass Spectrom 2007. :

» Chetyrkin, SV, Zhang, W, Hudson, BG, Serianni, AS, Voziyan, PA Pyridoxamine Protects Proteins from Functional Damage by 3-Deoxyglucosone: Mechanism of Action of Pyridoxamine Biochemistry 2007. :

» Fox, MA, Sanes, JR, Borza, DB, Eswarakumar, VP, F??ssler, R, Hudson, BG, John, SW, Ninomiya, Y, Pedchenko, V, Pfaff, SL, Rheault, MN, Sado, Y, Segal, Y, Werle, MJ, Umemori, H Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals Cell 2007. 129:179-93

» Steenhard, BM, Freeburg, PB, Isom, K, Stroganova, L, Borza, DB, Hudson, BG, St John, PL, Zelenchuk, A, Abrahamson, DR Kidney development and gene expression in the HIF2alpha knockout mouse Dev Dyn 2007. 236:1115-1125

» Kang, JS, Kashtan, CE, Turner, AN, Heidet, L, Hudson, BG, Borza, DB The Alloantigenic Sites of {alpha}3{alpha}4{alpha}5(IV) Collagen: PATHOGENIC X-LINKED ALPORT ALLOANTIBODIES TARGET TWO ACCESSIBLE CONFORMATIONAL EPITOPES IN THE {alpha}5NC1 DOMAIN J Biol Chem 2007. 282:10670-7

» Davies, SS, Brantley, EJ, Voziyan, PA, Amarnath, V, Zagol-Ikapitte, I, Boutaud, O, Hudson, BG, Oates, JA, Ii, LJ Pyridoxamine Analogues Scavenge Lipid-Derived gamma-Ketoaldehydes and Protect against H(2)O(2)-Mediated Cytotoxicity Biochemistry 2006. 45:15756-15767

» Zent, R, Yan, X, Su, Y, Hudson, BG, Borza, DB, Moeckel, GW, Qi, Z, Sado, Y, Breyer, MD, Voziyan, P, Pozzi, A Glomerular injury is exacerbated in diabetic integrin alpha1-null mice Kidney Int 2006. 70:460-70

» Miyoshi, T, Hirohata, S, Ogawa, H, Doi, M, Obika, M, Yonezawa, T, Sado, Y, Kusachi, S, Kyo, S, Kondo, S, Shiratori, Y, Hudson, BG, Ninomiya, Y Tumor-specific expression of the RGD-alpha3(IV)NC1 domain suppresses endothelial tube formation and tumor growth in mice FASEB J 2006. 20:1904-6

» Khoshnoodi, J, Cartailler, JP, Alvares, K, Veis, A, Hudson, BG Molecular recognition in the assembly of collagens: Terminal noncollagenous domains are key recognition modules in the formation of triple-helical protomers J Biol Chem 2006. :

» Harvey, SJ, Perry, J, Zheng, K, Chen, D, Sado, Y, Jefferson, B, Ninomiya, Y, Jacobs, R, Hudson, BG, Thorner, PS Sequential expression of type IV collagen networks: testis as a model and relevance to spermatogenesis Am J Pathol 2006. 168:1587-97

» Borza, CM, Pozzi, A, Borza, DB, Pedchenko, V, Hellmark, T, Hudson, BG, Zent, R Integrin alpha 3beta 1: a novel receptor for alpha 3 (IV) noncollagenous domain and a trans-dominant inhibitor for integrin alpha vbeta 3 J Biol Chem 2006. :

» Chen, L, Hellmark, T, Pedchenko, V, Hudson, BG, Pusey, CD, Fox, JW, Bolton, WK A Nephritogenic Peptide Induces Intermolecular Epitope Spreading on Collagen IV in Experimental Autoimmune Glomerulonephritis J Am Soc Nephrol 2006. 17:3076-81

» Khoshnoodi, J, Sigmundsson, K, Cartailler, JP, Bondar, O, Sundaramoorthy, M, Hudson, BG Mechanism of Chain Selection in the Assembly of Collagen IV: A PROMINENT ROLE FOR THE {alpha}2 CHAIN J Biol Chem 2006. 281:6058-69

» Chetyrkin, SV, Kim, D, Belmont, JM, Scheinman, JI, Hudson, BG, Voziyan, PA Pyridoxamine lowers kidney crystals in experimental hyperoxaluria: a potential therapy for primary hyperoxaluria Kidney Int 2005. 67:53-60

» Dean, EG, Wilson, GR, Li, M, Edgtton, KL, O''Sullivan, KM, Hudson, BG, Holdsworth, SR, Kitching, AR Experimental autoimmune Goodpasture''s disease: a pathogenetic role for both effector cells and antibody in injury Kidney Int 2005. 67:566-75

» Voziyan, PA, Hudson, BG Pyridoxamine as a multifunctional pharmaceutical: targeting pathogenic glycation and oxidative damage Cell Mol Life Sci 2005. 62:1671-81

» Borza, DB, Bondar, O, Colon, S, Todd, P, Sado, Y, Neilson, EG, Hudson, BG Goodpasture autoantibodies unmask cryptic epitopes by selectively dissociating autoantigen complexes lacking structural reinforcement: novel mechanisms for immune privilege and autoimmune pathogenesis J Biol Chem 2005. 280:27147-54

» Vanacore, RM, Friedman, DB, Ham, AJ, Sundaramoorthy, M, Hudson, BG Identification of S-hydroxylysyl-methionine as the covalent cross-link of the noncollagenous (NC1) hexamer of the alpha1alpha1alpha2 collagen IV network: a role for the post-translational modification of lysine 211 to hydroxylysine 211 in hexamer assembly J Biol Chem 2005. 280:29300-10

» Voziyan, PA, Hudson, BG Pyridoxamine: the many virtues of a maillard reaction inhibitor Ann N Y Acad Sci 2005. 1043:807-16

» Voziyan, PA, Chetyrkin, SV, Hudson, BG Amadori-lysyl-induced propagation of protein modifications is inhibited by pyridoxamine Ann N Y Acad Sci 2005. 1043:945

» Pedchenko, VK, Chetyrkin, SV, Chuang, P, Ham, AJ, Saleem, MA, Mathieson, PW, Hudson, BG, Voziyan, PA Mechanism of perturbation of integrin-mediated cell-matrix interactions by reactive carbonyl compounds and its implication for pathogenesis of diabetic nephropathy Diabetes 2005. 54:2952-60

» Zheng, K, Perry, J, Harvey, SJ, Sado, Y, Ninomiya, Y, Jefferson, B, Jacobs, R, Hudson, BG, Thorner, PS Regulation of collagen type IV genes is organ-specific: Evidence from a canine model of Alport syndrome Kidney Int 2005. 68:2121-30

» Scheinman, JI, Voziyan, PA, Belmont, JM, Chetyrkin, SV, Kim, D, Hudson, BG Pyridoxamine lowers oxalate excretion and kidney crystals in experimental hyperoxaluria: a potential therapy for primary hyperoxaluria Urol Res 2005. 33:368-71

» Hudson, BG The molecular basis of Goodpasture and Alport syndromes: beacons for the discovery of the collagen IV family J Am Soc Nephrol 2004. 15:2514-27

» Vanacore, RM, Shanmugasundararaj, S, Friedman, DB, Bondar, O, Hudson, BG, Sundaramoorthy, M The alpha1.alpha2 network of collagen IV. Reinforced stabilization of the noncollagenous domain-1 by noncovalent forces and the absence of Met-Lys cross-links J Biol Chem 2004. 279:44723-30

» Kitching, AR, Turner, AL, Semple, T, Li, M, Edgtton, KL, Wilson, GR, Timoshanko, JR, Hudson, BG, Holdsworth, SR Experimental autoimmune anti-glomerular basement membrane glomerulonephritis: a protective role for IFN-gamma J Am Soc Nephrol 2004. 15:1764-74

» Pedchenko, V, Zent, R, Hudson, BG Alpha(v)beta3 and alpha(v)beta5 integrins bind both the proximal RGD site and non-RGD motifs within noncollagenous (NC1) domain of the alpha3 chain of type IV collagen: implication for the mechanism of endothelia cell adhesion J Biol Chem 2004. 279:2772-80

» Hudson, BG, Tryggvason, K, Sundaramoorthy, M, Neilson, EG Alport''s syndrome, Goodpasture''s syndrome, and type IV collagen N Engl J Med 2003. 348:2543-56

» Borza, DB, Hudson, BG Molecular characterization of the target antigens of anti-glomerular basement membrane antibody disease Springer Semin Immunopathol 2003. 24:345-61

» Borza, DB, Neilson, EG, Hudson, BG Pathogenesis of Goodpasture syndrome: a molecular perspective Semin Nephrol 2003. 23:522-31

» Heidet, L, Borza, DB, Jouin, M, Sich, M, Mattei, MG, Sado, Y, Hudson, BG, Hastie, N, Antignac, C, Gubler, MC A human-mouse chimera of the alpha3alpha4alpha5(IV) collagen protomer rescues the renal phenotype in Col4a3-/- Alport mice Am J Pathol 2003. 163:1633-44

» Cussimanio, BL, Booth, AA, Todd, P, Hudson, BG, Khalifah, RG Unusual susceptibility of heme proteins to damage by glucose during non-enzymatic glycation Biophys Chem 2003. 105:743-55

» Voziyan, PA, Khalifah, RG, Thibaudeau, C, Yildiz, A, Jacob, J, Serianni, AS, Hudson, BG Modification of proteins in vitro by physiological levels of glucose: pyridoxamine inhibits conversion of Amadori intermediate to advanced glycation end-products through binding of redox metal ions J Biol Chem 2003. 278:46616-24

» Borza, DB, Bondar, O, Todd, P, Sundaramoorthy, M, Sado, Y, Ninomiya, Y, Hudson, BG Quaternary organization of the goodpasture autoantigen, the alpha 3(IV) collagen chain. Sequestration of two cryptic autoepitopes by intrapromoter interactions with the alpha4 and alpha5 NC1 domains J Biol Chem 2002. 277:40075-83

» Voziyan, PA, Metz, TO, Baynes, JW, Hudson, BG A post-Amadori inhibitor pyridoxamine also inhibits chemical modification of proteins by scavenging carbonyl intermediates of carbohydrate and lipid degradation J Biol Chem 2002. 277:3397-403

» Sundaramoorthy, M, Meiyappan, M, Todd, P, Hudson, BG Crystal structure of NC1 domains. Structural basis for type IV collagen assembly in basement membranes J Biol Chem 2002. 277:31142-53

» Borza, DB, Hudson, BG Of mice and men: murine models of anti-GBM antibody nephritis Kidney Int 2002. 61:1905-6

» Borza, DB, Bondar, O, Ninomiya, Y, Sado, Y, Naito, I, Todd, P, Hudson, BG The NC1 domain of collagen IV encodes a novel network composed of the alpha 1, alpha 2, alpha 5, and alpha 6 chains in smooth muscle basement membranes J Biol Chem 2001. 276:28532-40

» David, M, Borza, DB, Leinonen, A, Belmont, JM, Hudson, BG Hydrophobic amino acid residues are critical for the immunodominant epitope of the Goodpasture autoantigen. A molecular basis for the cryptic nature of the epitope J Biol Chem 2001. 276:6370-7

» Petitclerc, E, Boutaud, A, Prestayko, A, Xu, J, Sado, Y, Ninomiya, Y, Sarras, MP, Hudson, BG, Brooks, PC New functions for non-collagenous domains of human collagen type IV. Novel integrin ligands inhibiting angiogenesis and tumor growth in vivo J Biol Chem 2000. 275:8051-61

» Borza, DB, Netzer, KO, Leinonen, A, Todd, P, Cervera, J, Saus, J, Hudson, BG The goodpasture autoantigen. Identification of multiple cryptic epitopes on the NC1 domain of the alpha3(IV) collagen chain J Biol Chem 2000. 275:6030-7

» Ghohestani, RF, Hudson, BG, Claudy, A, Uitto, J The alpha 5 chain of type IV collagen is the target of IgG autoantibodies in a novel autoimmune disease with subepidermal blisters and renal insufficiency J Biol Chem 2000. 275:16002-6

» Boutaud, A, Borza, DB, Bondar, O, Gunwar, S, Netzer, KO, Singh, N, Ninomiya, Y, Sado, Y, Noelken, ME, Hudson, BG Type IV collagen of the glomerular basement membrane. Evidence that the chain specificity of network assembly is encoded by the noncollagenous NC1 domains J Biol Chem 2000. 275:30716-24

» Khalifah, RG, Baynes, JW, Hudson, BG Amadorins: novel post-Amadori inhibitors of advanced glycation reactions Biochem Biophys Res Commun 1999. 257:251-8

» Leinonen, A, Netzer, KO, Boutaud, A, Gunwar, S, Hudson, BG Goodpasture antigen: expression of the full-length alpha3(IV) chain of collagen IV and localization of epitopes exclusively to the noncollagenous domain Kidney Int 1999. 55:926-35

» Netzer, KO, Leinonen, A, Boutaud, A, Borza, DB, Todd, P, Gunwar, S, Langeveld, JP, Hudson, BG The goodpasture autoantigen. Mapping the major conformational epitope(s) of alpha3(IV) collagen to residues 17-31 and 127-141 of the NC1 domain J Biol Chem 1999. 274:11267-74

» Fervenza, FC, Terreros, D, Boutaud, A, Hudson, BG, Williams, RA, Donadio, JV, Schwab, TR Recurrent Goodpasture''s disease due to a monoclonal IgA-kappa circulating antibody Am J Kidney Dis 1999. 34:549-55

» Harvey, SJ, Zheng, K, Sado, Y, Naito, I, Ninomiya, Y, Jacobs, RM, Hudson, BG, Thorner, PS Role of distinct type IV collagen networks in glomerular development and function Kidney Int 1998. 54:1857-66

» Abbate, M, Kalluri, R, Corna, D, Yamaguchi, N, McCluskey, RT, Hudson, BG, Andres, G, Zoja, C, Remuzzi, G Experimental Goodpasture''s syndrome in Wistar-Kyoto rats immunized with alpha3 chain of type IV collagen Kidney Int 1998. 54:1550-61

» Kalluri, R, Gattone, VH, Hudson, BG Identification and localization of type IV collagen chains in the inner ear cochlea Connect Tissue Res 1998. 37:143-50

» Gunwar, S, Ballester, F, Noelken, ME, Sado, Y, Ninomiya, Y, Hudson, BG Glomerular basement membrane. Identification of a novel disulfide-cross-linked network of alpha3, alpha4, and alpha5 chains of type IV collagen and its implications for the pathogenesis of Alport syndrome J Biol Chem 1998. 273:8767-75

» Sado, Y, Boutaud, A, Kagawa, M, Naito, I, Ninomiya, Y, Hudson, BG Induction of anti-GBM nephritis in rats by recombinant alpha 3(IV)NC1 and alpha 4(IV)NC1 of type IV collagen Kidney Int 1998. 53:664-71

» Netzer, KO, Suzuki, K, Itoh, Y, Hudson, BG, Khalifah, RG Comparative analysis of the noncollagenous NC1 domain of type IV collagen: identification of structural features important for assembly, function, and pathogenesis Protein Sci 1998. 7:1340-51

» Kalluri, R, Shield, CF, Todd, P, Hudson, BG, Neilson, EG Isoform switching of type IV collagen is developmentally arrested in X-linked Alport syndrome leading to increased susceptibility of renal basement membranes to endoproteolysis J Clin Invest 1997. 99:2470-8

» Booth, AA, Khalifah, RG, Todd, P, Hudson, BG In vitro kinetic studies of formation of antigenic advanced glycation end products (AGEs). Novel inhibition of post-Amadori glycation pathways J Biol Chem 1997. 272:5430-7

» Mott, JD, Khalifah, RG, Nagase, H, Shield, CF, Hudson, JK, Hudson, BG Nonenzymatic glycation of type IV collagen and matrix metalloproteinase susceptibility Kidney Int 1997. 52:1302-12

» Kahsai, TZ, Enders, GC, Gunwar, S, Brunmark, C, Wieslander, J, Kalluri, R, Zhou, J, Noelken, ME, Hudson, BG Seminiferous tubule basement membrane. Composition and organization of type IV collagen chains, and the linkage of alpha3(IV) and alpha5(IV) chains J Biol Chem 1997. 272:17023-32

» Kalluri, R, Sun, MJ, Hudson, BG, Neilson, EG The Goodpasture autoantigen. Structural delineation of two immunologically privileged epitopes on alpha3(IV) chain of type IV collagen J Biol Chem 1996. 271:9062-8

» Khalifah, RG, Todd, P, Booth, AA, Yang, SX, Mott, JD, Hudson, BG Kinetics of nonenzymatic glycation of ribonuclease A leading to advanced glycation end products. Paradoxical inhibition by ribose leads to facile isolation of protein intermediate for rapid post-Amadori studies Biochemistry 1996. 35:4645-54

» Cheung, WF, van den Born, J, K??hn, K, Kjell??n, L, Hudson, BG, Stafford, DW Identification of the endothelial cell binding site for factor IX Proc Natl Acad Sci U S A 1996. 93:11068-73

» Thorner, PS, Zheng, K, Kalluri, R, Jacobs, R, Hudson, BG Coordinate gene expression of the alpha3, alpha4, and alpha5 chains of collagen type IV. Evidence from a canine model of X-linked nephritis with a COL4A5 gene mutation J Biol Chem 1996. 271:13821-8

» Boutaud, AA, Kalluri, R, Kahsai, TZ, Noelken, ME, Hudson, BG Goodpasture syndrome: selective removal of anti-alpha 3 (IV) collagen autoantibodies. A potential therapeutic alternative to plasmapheresis Exp Nephrol 1996. 4:205-12

» Booth, AA, Khalifah, RG, Hudson, BG Thiamine pyrophosphate and pyridoxamine inhibit the formation of antigenic advanced glycation end-products: comparison with aminoguanidine Biochem Biophys Res Commun 1996. 220:113-9

» Merkel, F, Kalluri, R, Marx, M, Enders, U, Stevanovic, S, Giegerich, G, Neilson, EG, Rammensee, HG, Hudson, BG, Weber, M Autoreactive T-cells in Goodpasture''s syndrome recognize the N-terminal NC1 domain on alpha 3 type IV collagen Kidney Int 1996. 49:1127-33

» Enders, GC, Kahsai, TZ, Lian, G, Funabiki, K, Killen, PD, Hudson, BG Developmental changes in seminiferous tubule extracellular matrix components of the mouse testis: alpha 3(IV) collagen chain expressed at the initiation of spermatogenesis Biol Reprod 1995. 53:1489-99

» Peissel, B, Geng, L, Kalluri, R, Kashtan, C, Rennke, HG, Gallo, GR, Yoshioka, K, Sun, MJ, Hudson, BG, Neilson, EG Comparative distribution of the alpha 1(IV), alpha 5(IV), and alpha 6(IV) collagen chains in normal human adult and fetal tissues and in kidneys from X-linked Alport syndrome patients J Clin Invest 1995. 96:1948-57

» Kalluri, R, Wilson, CB, Weber, M, Gunwar, S, Chonko, AM, Neilson, EG, Hudson, BG Identification of the alpha 3 chain of type IV collagen as the common autoantigen in antibasement membrane disease and Goodpasture syndrome J Am Soc Nephrol 1995. 6:1178-85

» Kalluri, R, van den Heuvel, LP, Smeets, HJ, Schroder, CH, Lemmink, HH, Boutaud, A, Neilson, EG, Hudson, BG A COL4A3 gene mutation and post-transplant anti-alpha 3(IV) collagen alloantibodies in Alport syndrome Kidney Int 1995. 47:1199-204

» Noelken, ME, Reddy, GK, Kalluri, R, Gunwar, S, Hudson, BG The chain composition of aorta basement membrane collagen IV, localization of the chains in aorta and supramolecular assembly of the collagen IV protomers Contrib Nephrol 1994. 107:216-9

» Baynes, RD, Shih, YJ, Hudson, BG, Cook, JD Identification of the membrane remnants of transferrin receptor with domain-specific antibodies J Lab Clin Med 1994. 123:407-14

» Kalluri, R, Weber, M, Netzer, KO, Sun, MJ, Neilson, EG, Hudson, BG COL4A5 gene deletion and production of post-transplant anti-alpha 3(IV) collagen alloantibodies in Alport syndrome Kidney Int 1994. 45:721-6

» Hudson, BG, Kalluri, R, Tryggvason, K Pathology of glomerular basement membrane nephropathy Curr Opin Nephrol Hypertens 1994. 3:334-9

» Kalluri, R, Gattone, VH, Noelken, ME, Hudson, BG The alpha 3 chain of type IV collagen induces autoimmune Goodpasture syndrome Proc Natl Acad Sci U S A 1994. 91:6201-5

» Zhang, X, Hudson, BG, Sarras, MP Hydra cell aggregate development is blocked by selective fragments of fibronectin and type IV collagen Dev Biol 1994. 164:10-23

» Hudson, BG, Kalluri, R, Gunwar, S, Noelken, ME Structure and organization of type IV collagen of renal glomerular basement membrane Contrib Nephrol 1994. 107:163-7

» Hudson, BG, Reeders, ST, Tryggvason, K Type IV collagen: structure, gene organization, and role in human diseases. Molecular basis of Goodpasture and Alport syndromes and diffuse leiomyomatosis J Biol Chem 1993. 268:26033-6

» Baynes, RD, Shih, YJ, Hudson, BG, Cook, JD Production of the serum form of the transferrin receptor by a cell membrane-associated serine protease Proc Soc Exp Biol Med 1993. 204:65-9

» Reddy, GK, Gunwar, S, Kalluri, R, Hudson, BG, Noelken, ME Structure and composition of type IV collagen of bovine aorta Biochim Biophys Acta 1993. 1157:241-51

» Neilson, EG, Kalluri, R, Sun, MJ, Gunwar, S, Danoff, T, Mariyama, M, Myers, JC, Reeders, ST, Hudson, BG Specificity of Goodpasture autoantibodies for the recombinant noncollagenous domains of human type IV collagen J Biol Chem 1993. 268:8402-5

» Reddy, GK, Hudson, BG, Bailey, AJ, Noelken, ME Reductive cleavage of the disulfide bonds of the collagen IV noncollagenous domain in aqueous sodium dodecyl sulfate: absence of intermolecular nondisulfide cross-links Biochem Biophys Res Commun 1993. 190:277-82

» Hudson, BG, Kalluri, R, Gunwar, S, Noelken, ME, Mariyama, M, Reeders, ST Molecular characteristics of the Goodpasture autoantigen Kidney Int 1993. 43:135-9

» Shih, YJ, Baynes, RD, Hudson, BG, Cook, JD Characterization and quantitation of the circulating forms of serum transferrin receptor using domain-specific antibodies Blood 1993. 81:234-8

» Mariyama, M, Kalluri, R, Hudson, BG, Reeders, ST The alpha 4(IV) chain of basement membrane collagen. Isolation of cDNAs encoding bovine alpha 4(IV) and comparison with other type IV collagens J Biol Chem 1992. 267:1253-8

» Hudson, BG, Kalluri, R, Gunwar, S, Weber, M, Ballester, F, Hudson, JK, Noelken, ME, Sarras, M, Richardson, WR, Saus, J The pathogenesis of Alport syndrome involves type IV collagen molecules containing the alpha 3(IV) chain: evidence from anti-GBM nephritis after renal transplantation Kidney Int 1992. 42:179-87

» Wisdom, BJ, Gunwar, S, Hudson, MD, Noelken, ME, Hudson, BG Type IV collagen of Engelbreth-Holm-Swarm tumor matrix: identification of constituent chains Connect Tissue Res 1992. 27:225-34

» Kalluri, R, Gunwar, S, Reeders, ST, Morrison, KC, Mariyama, M, Ebner, KE, Noelken, ME, Hudson, BG Goodpasture syndrome. Localization of the epitope for the autoantibodies to the carboxyl-terminal region of the alpha 3(IV) chain of basement membrane collagen J Biol Chem 1991. 266:24018-24

» Langeveld, JP, Noelken, ME, H?Yrd, K, Todd, P, Vliegenthart, JF, Rouse, J, Hudson, BG Bovine glomerular basement membrane. Location and structure of the asparagine-linked oligosaccharide units and their potential role in the assembly of the 7 S collagen IV tetramer J Biol Chem 1991. 266:2622-31

» Gunwar, S, Noelken, ME, Hudson, BG Properties of the collagenous domain of the alpha 3(IV) chain, the Goodpasture antigen, of lens basement membrane collagen. Selective cleavage of alpha (IV) chains with retention of their triple helical structure and noncollagenous domain J Biol Chem 1991. 266:14088-94

» Gunwar, S, Bejarano, PA, Kalluri, R, Langeveld, JP, Wisdom, BJ, Noelken, ME, Hudson, BG Alveolar basement membrane: molecular properties of the noncollagenous domain (hexamer) of collagen IV and its reactivity with Goodpasture autoantibodies Am J Respir Cell Mol Biol 1991. 5:107-12

» Gunwar, S, Ballester, F, Kalluri, R, Timoneda, J, Chonko, AM, Edwards, SJ, Noelken, ME, Hudson, BG Glomerular basement membrane. Identification of dimeric subunits of the noncollagenous domain (hexamer) of collagen IV and the Goodpasture antigen J Biol Chem 1991. 266:15318-24

» Sarras, MP, Madden, ME, Zhang, XM, Gunwar, S, Huff, JK, Hudson, BG Extracellular matrix (mesoglea) of Hydra vulgaris. I. Isolation and characterization Dev Biol 1991. 148:481-94

» Baynes, RD, Shih, YJ, Hudson, BG, Cook, JD Characterization of transferrin receptor released by K562 erythroleukemia cells Proc Soc Exp Biol Med 1991. 197:416-23

» Fagg, WR, Timoneda, J, Schwartz, CE, Langeveld, JP, Noelken, ME, Hudson, BG Glomerular basement membrane: evidence for collagenous domain of the alpha 3 and alpha 4 chains of collagen IV Biochem Biophys Res Commun 1990. 170:322-7

» Timoneda, J, Gunwar, S, Monfort, G, Saus, J, Noelken, ME, Hudson, BG Unusual dissociative behavior of the noncollagenous domain (hexamer) of basement membrane collagen during electrophoresis and chromatofocusing Connect Tissue Res 1990. 24:169-86

» Zhang, X, Huff, JK, Hudson, BG, Sarras, MP A non-mammalian in vivo model for cellular and molecular analysis of glucose-mediated thickening of basement membranes Diabetologia 1990. 33:704-7

» Shih, YJ, Baynes, RD, Hudson, BG, Flowers, CH, Skikne, BS, Cook, JD Serum transferrin receptor is a truncated form of tissue receptor J Biol Chem 1990. 265:19077-81

» Gunwar, S, Saus, J, Noelken, ME, Hudson, BG Glomerular basement membrane. Identification of a fourth chain, alpha 4, of type IV collagen J Biol Chem 1990. 265:5466-9

» Hudson, BG, Wieslander, J, Wisdom, BJ, Noelken, ME Goodpasture syndrome: molecular architecture and function of basement membrane antigen Lab Invest 1989. 61:256-69

» Bejarano, PA, Langeveld, JP, Hudson, BG, Noelken, ME Degradation of basement membranes by Pseudomonas aeruginosa elastase Infect Immun 1989. 57:3783-7

» Langeveld, JP, Wieslander, J, Timoneda, J, McKinney, P, Butkowski, RJ, Wisdom, BJ, Hudson, BG Structural heterogeneity of the noncollagenous domain of basement membrane collagen J Biol Chem 1988. 263:10481-8

» Saus, J, Wieslander, J, Langeveld, JP, Quinones, S, Hudson, BG Identification of the Goodpasture antigen as the alpha 3(IV) chain of collagen IV J Biol Chem 1988. 263:13374-80

» Bejarano, PA, Noelken, ME, Suzuki, K, Hudson, BG, Nagase, H Degradation of basement membranes by human matrix metalloproteinase 3 (stromelysin) Biochem J 1988. 256:413-9

» Butkowski, RJ, Langeveld, JP, Wieslander, J, Hamilton, J, Hudson, BG Localization of the Goodpasture epitope to a novel chain of basement membrane collagen J Biol Chem 1987. 262:7874-7

» Wieslander, J, Kataja, M, Hudson, BG Characterization of the human Goodpasture antigen Clin Exp Immunol 1987. 69:332-40

» Noelken, ME, Wisdom, BJ, Dean, DC, Hung, CH, Hudson, BG Intestinal basement membrane of Ascaris suum. Molecular organization and properties of the collagen molecules J Biol Chem 1986. 261:4706-14

» Wieslander, J, Langeveld, J, Butkowski, R, Jodlowski, M, Noelken, M, Hudson, BG Physical and immunochemical studies of the globular domain of type IV collagen. Cryptic properties of the Goodpasture antigen J Biol Chem 1985. 260:8564-70

» Butkowski, RJ, Carone, FA, Grantham, JJ, Hudson, BG Tubular basement membrane changes in 2-amino-4,5-diphenylthiazole-induced polycystic disease Kidney Int 1985. 28:744-51

» Butkowski, RJ, Wieslander, J, Wisdom, BJ, Barr, JF, Noelken, ME, Hudson, BG Properties of the globular domain of type IV collagen and its relationship to the Goodpasture antigen J Biol Chem 1985. 260:3739-47

» Wieslander, J, Barr, JF, Butkowski, RJ, Edwards, SJ, Bygren, P, Heineg?Yrd, D, Hudson, BG Goodpasture antigen of the glomerular basement membrane: localization to noncollagenous regions of type IV collagen Proc Natl Acad Sci U S A 1984. 81:3838-42

» Dean, DC, Barr, JF, Freytag, JW, Hudson, BG Isolation of type IV procollagen-like polypeptides from glomerular basement membrane. Characterization of pro-alpha 1(IV) J Biol Chem 1983. 258:590-6

» Brieden, HC, Good, ED, Hudson, BG, Mishelevich, DJ, Mize, EI, Robinson, AL, Rogers, C The Parkland On-Line Information System (POIS) associate concept Comput Healthc 1983. 4:40-4

» Hung, CH, Noelken, ME, Hudson, BG Intestinal basement membrane of Ascaris suum. Physical properties of the collagenous domain J Biol Chem 1981. 256:3822-6

» Dean, DC, Peczon, BD, Noelken, ME, Hudson, BG Bovine glomerular basement membrane. Characterization of an alpha-size collagenous polypeptide J Biol Chem 1981. 256:7543-8

» Noelken, ME, Wisdom, BJ, Hudson, BG Estimation of the size of collagenous polypeptides by sodium dodecyl sulfate--polyacrylamide gel electrophoresis Anal Biochem 1981. 110:131-6

» Fox, JW, Butkowski, RJ, Hudson, BG Detergent-prepared glomerular basement membrane is composed of a heterogeneous group of polypeptides J Biol Chem 1981. 256:9313-5

» Mishelevich, DJ, Kesinger, G, Jasper, M, Inga, P, Robinson, AL, Gaige, W, Hudson, BG, Tyler, N Medical record control and the computer Top Health Rec Manage 1981. 2:47-55

» Butkowski, RJ, Brungardt, GS, Grantham, JJ, Hudson, BG Characterization of the collagenous domain of tubular basement membrane J Biol Chem 1981. 256:7603-9

» West, TW, Fox, JW, Jodlowski, M, Freytag, JW, Hudson, BG Bovine glomerular basement membrane. Properties of the collagenous domain J Biol Chem 1980. 255:10451-9

» Hung, CH, Butkowski, RJ, Hudson, BG Intestinal basement membrane of Ascaris suum. Properties of the collagenous domain J Biol Chem 1980. 255:4964-71

» Butkowski, RJ, Grantham, JJ, Hudson, BG Comparison of the chemical and polypeptide composition of tubular and glomerular basement membranes Ren Physiol 1980. 3:14-22

» Hudson, BG, Fox, JW Characterization of the collagenous domain of bovine glomerular basement membrane Ren Physiol 1980. 3:9-13

» Prasad, R, Hudson, BG, Strickland, DK, Ebner, KE The structure of the asparagine-linked carbohydrate unit of rat alpha-lactalbumin J Biol Chem 1980. 255:1248-51

» Peczon, BD, Peczon, JD, Cintron, C, Hudson, BG Changes in chemical composition of anterior lens capsules of cataractous human eyes as a function of age Exp Eye Res 1980. 30:155-65

» Butkowski, RJ, Todd, P, Grantham, JJ, Hudson, BG Rabbit tubular basement membrane. Isolation and analysis of polypeptides J Biol Chem 1979. 254:10503-13

» Leung, CC, Hung, CH, Hudson, BG, Brent, RL, Cotton, RC Congenital abnormalities induced by heterologous antisera directed against rat kidney glycoproteins isolated by concanavalin A affinity chromatography Pediatr Res 1979. 13:1211-6

» Prasad, R, Hudson, BG, Butkowski, R, Hamilton, JW, Ebner, KE Resolution of the charge forms and amino acid sequence and location of a tryptic glycopeptide in rat alpha-lactalbumin J Biol Chem 1979. 254:10607-14

» Davis, JL, Chandler, DK, Riquetti, P, Rogers, S, Kocan, KM, Ownby, CL, Hudson, BG, Carraway, KL Interaction of bovine platelets with bovine glomerular basement membrane Thromb Haemost 1979. 42:1217-29

» Freytag, JW, Noelken, ME, Hudson, BG Physical properties of collagen--sodium dodecyl sulfate complexes Biochemistry 1979. 18:4761-8

» Strickland, DK, Hamilton, JW, Hudson, BG Structure of the tryptic glycopeptide isolated from rabbit transferrin Biochemistry 1979. 18:2549-54

» Freytag, JW, Dalrymple, PN, Maguire, MH, Strickland, DK, Carraway, KL, Hudson, BG Glomerular basement membrane. Studies on its structure and interaction with platelets J Biol Chem 1978. 253:9069-74

» Strickland, DK, Hudson, BG Structural studies on rabbit transferrin: isolation and characterization of the glycopeptides Biochemistry 1978. 17:3411-8

» Freytag, JW, Ohno, M, Hudson, BG Large scale preparation of bovine renal glomerular basement membrane in the presence of protease inhibitors Prep Biochem 1978. 8:215-24

» Brown, RC, Fish, WW, Hudson, BG, Ebner, KE Isolation and characterization of rat alpha-lactalbumin: a glycoprotein Biochim Biophys Acta 1977. 491:82-92

» Hung, CH, Strickland, DK, Hudson, BG Estimation of molecular weights of peptides and glycopeptides by thin-layer gel filtration Anal Biochem 1977. 80:91-100

» Hung, CH, Ohno, M, Freytag, JW, Hudson, BG Intestinal basement membrane of Ascaris suum. Analysis of polypeptide components J Biol Chem 1977. 252:3995-4001

» Peczon, BD, Wegener, LJ, Hung, CH, Hudson, BG Intestinal basement membrane of Ascaris suum. Characterization of carbohydrate units J Biol Chem 1977. 252:4002-6

» Tsao, D, Colton, DG, Chang, JS, Buck, RL, Hudson, BG, Carraway, KL Alterations of red cell membranes from phenylhydrazine-treated rabbits Biochim Biophys Acta 1977. 469:61-73

» Freytag, JW, Ohno, M, Hudson, BG Bovine renal glomerular basement membrane. Assessment of proteolysis during isolation Biochem Biophys Res Commun 1976. 72:796-802

» Lehman, ED, Hudson, BG, Ebner, KE Studies on the carbohydrate structure of bovine milk galactosyltransferase FEBS Lett 1975. 54:65-9

» Hudson, BG, Wegener, LJ, Wingate, JM, Carraway, KL Chemical studies of erythrocyte membrane glycoproteins from several species Comp Biochem Physiol B 1975. 51:127-35

» Shin, BC, Ebner, KE, Hudson, BG, Carraway, KL Membrane glycoprotein differences between normal lactating mammary tissue and the R3230 AC mammary tumor Cancer Res 1975. 35:1135-40

» Hudson, BG, Heldebrant, CM, Mann, KG Distribution of prothrombin carbohydrate units upon thrombin activation Thromb Res 1975. 6:215-21

» Oho, M, Riquetti, P, Hudson, BG Bovine renal glomerular basement membrane. Isolation and characterization of a glycoprotein component J Biol Chem 1975. 250:7780-7

» Guire, P, Riquetti, P, Hudson, BG An accelerated single column procedure for the programmed analysis of naturally-occuring amino acids of collagen and basement membrane J Chromatogr 1974. 90:350-3

» Odell, V, Wegener, L, Peczon, B, Hudson, BG A rapid automatic analysis of hydroxylysine, hydroxylysine glycosides, and methionine sulfoxide in collagen and basement membrane J Chromatogr 1974. 88:245-52

» Lehman, ED, Hudson, BG, Ebner, KE Micro-chromatographic and radiochemical analysis of glycopeptides J Chromatogr 1973. 76:345-50

» Hudson, BG, Ono, M, Brockway, WJ, Castellino, FJ Chemical and physical properties of serum transferrins from several species Biochemistry 1973. 12:1047-53

» Hudson, BG, McKenzie, L, Ebner, KE Enzymic synthesis of lactose- 14 C (galactosyl- 14 C) and lactose- 3 H (glucosyl- 3 H) Anal Biochem 1972. 48:524-8

» Hudson, BG, Spiro, RG Fractionation of glycoprotein components of the reduced alkylated renal glomerular basement membrane J Biol Chem 1972. 247:4239-47

» Hudson, BG, Spiro, RG Studies on the native and reduced alkylated renal glomerular basement membrane. Solubility, subunit size, and reaction with cyanogen bromide J Biol Chem 1972. 247:4229-38

» Zipilivan, EM, Hudson, BG, Blatt, WF Separation of immunologically active fragments by membrane partition chromatography Anal Biochem 1969. 30:91-8

» Blatt, WF, Hudson, BG Removal of low molecular weight contaminants from 131-I-labeled insulin by selective ultrafiltration Anal Biochem 1968. 26:329-33

» Blatt, WF, Hudson, BG, Robinson, SM, Zipilivan, EM A modified ultrafiltration cell for separating the products of proteolysis Anal Biochem 1968. 22:161-5

» Blatt, WF, Hudson, BG, Robinson, SM, Zipilivan, EM Fractionation of protein solutions by membrane partition chromatography Nature 1967. 216:511-3

» Hudson, BG, Barker, R The overoxidation of carbohydrates with sodium metaperiodate J Org Chem 1967. 32:2101-9