Center for Matrix Biology - Vanderbilt University and Medical Center

Steven K. Hanks, Ph.D.
Professor in the Department of Cell and Developmental Biology

	Office Address:	U-4200 MCN
	Mailing Address:	U-4200 MCN Nashville, TN 8240
	Lab address:	U-4206 Learned Labs, MRB-III
	Phone:	615-343-8502
		email

Secondary appointment in the Medicine department.

Research Summary

Our broad research goals are to understand the molecular events that regulate mammalian cell growth, differentiation, and motility, and to determine how these events go awry in cancer and other human diseases. Our primary focus is on tyrosine phosphorylation signaling events regulated by cell-ECM interactions and their roles in cell motility and invasion.

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Associated initiative(s):

Diabetic Complications

Wound Repair

Career opportunities:
None currently available

Selected Publications

» Siesser, PM, Meenderink, LM, Ryzhova, L, Michael, KE, Dumbauld, DW, Garc?-a, AJ, Kaverina, I, Hanks, SK A FAK/Src chimera with gain-of-function properties promotes formation of large peripheral adhesions associated with dynamic actin assembly Cell Motil Cytoskeleton 2008. 65:25-39

» Luo, W, Slebos, RJ, Hill, S, Li, M, BraI?bek, J, Amanchy, R, Chaerkady, R, Pandey, A, Ham, AJ, Hanks, SK Global Impact of Oncogenic Src on a Phosphotyrosine Proteome J Proteome Res 2008. :

» Kim, W, Seok Kang, Y, Soo Kim, J, Shin, NY, Hanks, SK, Song, WK The Integrin-coupled Signaling Adaptor p130Cas Suppresses Smad3 Function in Transforming Growth Factor-{beta} Signaling Mol Biol Cell 2008. 19:2135-46

» Constancio-Lund, SS, Brabek, J, Hanks, SK Src transformation of colonic epithelial cells: Enhanced anchorage-independent growth in an Apc(+/min) background Mol Carcinog 2008. :

» Liu, G, Li, W, Gao, X, Li, X, J??rgensen, C, Park, HT, Shin, NY, Yu, J, He, ML, Hanks, SK, Wu, JY, Guan, KL, Rao, Y p130CAS is required for netrin signaling and commissural axon guidance J Neurosci 2007. 27:957-68

» Frey, MT, Tsai, IY, Russell, TP, Hanks, SK, Wang, YL Cellular responses to substrate topography: role of myosin II and focal adhesion kinase Biophys J 2006. 90:3774-82

» Siesser, PM, Hanks, SK The signaling and biological implications of FAK overexpression in cancer Clin Cancer Res 2006. 12:3233-7

» Rivera, GM, Antoku, S, Gelkop, S, Shin, NY, Hanks, SK, Pawson, T, Mayer, BJ Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B Proc Natl Acad Sci U S A 2006. 103:9536-41

» Bianchi, M, De Lucchini, S, Marin, O, Turner, DL, Hanks, SK, Villa-Moruzzi, E Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration Biochem J 2005. 391:359-70

» Cai, S, Bulus, N, Fonseca-Siesser, PM, Chen, D, Hanks, SK, Pozzi, A, Zent, R CD98 modulates integrin beta1 function in polarized epithelial cells J Cell Sci 2005. 118:889-99

» Br?!bek, J, Constancio, SS, Siesser, PF, Shin, NY, Pozzi, A, Hanks, SK Crk-associated substrate tyrosine phosphorylation sites are critical for invasion and metastasis of SRC-transformed cells Mol Cancer Res 2005. 3:307-15

» Shin, Nah-Young, Dise, Rebecca S, Schneider-Mergener, Jens, Ritchie, Marylyn D, Kilkenny, Dawn M, Hanks, Steven K Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration J Biol Chem 2004. 279:38331-7

» Br?!bek, Jan, Hanks, Steven K Assaying protein kinase activity Methods Mol Biol 2004. 284:79-90

» Wang, YL, Burridge, K, Dembo, M, Gabbiani, G, Hanks, SK, Hosoya, H, Janmey, P, Karlsson, R, Lindberg, U, Mabuchi, I, Otey, C, Rottner, K, Small, JV, Wang, CL, Zigmond, S Biomedical research publication system Science 2004. 303:1974-6

» Yano, H, Mazaki, Y, Kurokawa, K, Hanks, SK, Matsuda, M, Sabe, H Roles played by a subset of integrin signaling molecules in cadherin-based cell-cell adhesion J Cell Biol 2004. 166:283-95

» Br?!bek, Jan, Constancio, S?!bata S, Shin, Nah-Young, Pozzi, Ambra, Weaver, Alissa M, Hanks, Steven K CAS promotes invasiveness of Src-transformed cells Oncogene 2004. 23:7406-15

» Hecker, Timothy P, Ding, Qiang, Rege, Tanya A, Hanks, Steven K, Gladson, Candece L Overexpression of FAK promotes Ras activity through the formation of a FAK/p120RasGAP complex in malignant astrocytoma cells Oncogene 2004. 23:3962-71

» Kurenova, Elena, Xu, Li-Hui, Yang, Xihui, Baldwin, Albert S, Craven, Rolf J, Hanks, Steven K, Liu, Zheng-Gang, Cance, William G Focal adhesion kinase suppresses apoptosis by binding to the death domain of receptor-interacting protein Mol Cell Biol 2004. 24:4361-71

» Fonseca, Priscila M, Shin, Nah-Young, Br?!bek, Jan, Ryzhova, Larisa, Wu, Jiong, Hanks, Steven K Regulation and localization of CAS substrate domain tyrosine phosphorylation Cell Signal 2004. 16:621-9

» Torsoni, Adriana S, Constancio, Sabata S, Nadruz, Wilson, Hanks, Steven K, Franchini, Kleber G Focal adhesion kinase is activated and mediates the early hypertrophic response to stretch in cardiac myocytes Circ Res 2003. 93:140-7

» Hanks, Steven K, Ryzhova, Larisa, Shin, Nah-Young, Br?!bek, Jan Focal adhesion kinase signaling activities and their implications in the control of cell survival and motility Front Biosci 2003. 8:d982-96

» Hanks, Steven K Genomic analysis of the eukaryotic protein kinase superfamily: a perspective Genome Biol 2003. 4:111

» Li, Guochun, Gustafson-Brown, Cindy, Hanks, Steven K, Nason, Katie, Arbeit, Jeffrey M, Pogliano, Kit, Wisdom, Ronald M, Johnson, Randall S c-Jun is essential for organization of the epidermal leading edge Dev Cell 2003. 4:865-77

» Miller, Michael A, Ruest, Paul J, Kosinski, Mary, Hanks, Steven K, Greenstein, David An Eph receptor sperm-sensing control mechanism for oocyte meiotic maturation in Caenorhabditis elegans Genes Dev 2003. 17:187-200

» Roy, Shyamali, Ruest, Paul J, Hanks, Steven K FAK regulates tyrosine phosphorylation of CAS, paxillin, and PYK2 in cells expressing v-Src, but is not a critical determinant of v-Src transformation J Cell Biochem 2002. 84:377-88

» Donaldson, John C, Dise, Rebecca S, Ritchie, Marylyn D, Hanks, Steven K Nephrocystin-conserved domains involved in targeting to epithelial cell-cell junctions, interaction with filamins, and establishing cell polarity J Biol Chem 2002. 277:29028-35

» Wang, H B, Dembo, M, Hanks, S K, Wang, Y Focal adhesion kinase is involved in mechanosensing during fibroblast migration Proc Natl Acad Sci U S A 2001. 98:11295-300

» Ruest, P J, Shin, N Y, Polte, T R, Zhang, X, Hanks, S K Mechanisms of CAS substrate domain tyrosine phosphorylation by FAK and Src Mol Cell Biol 2001. 21:7641-52

» Maru, Y, Hanks, S K, Shibuya, M The tubulogenic activity associated with an activated form of Flt-1 kinase is dependent on focal adhesion kinase Biochim Biophys Acta 2001. 1540:147-53

» Sonoda, Y, Matsumoto, Y, Funakoshi, M, Yamamoto, D, Hanks, S K, Kasahara, T Anti-apoptotic role of focal adhesion kinase (FAK). Induction of inhibitor-of-apoptosis proteins and apoptosis suppression by the overexpression of FAK in a human leukemic cell line, HL-60 J Biol Chem 2000. 275:16309-15

» Donaldson, J C, Dempsey, P J, Reddy, S, Bouton, A H, Coffey, R J, Hanks, S K Crk-associated substrate p130(Cas) interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells Exp Cell Res 2000. 256:168-78

» Ruest, P J, Roy, S, Shi, E, Mernaugh, R L, Hanks, S K Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site Cell Growth Differ 2000. 11:41-8

» Yamakita, Y, Totsukawa, G, Yamashiro, S, Fry, D, Zhang, X, Hanks, S K, Matsumura, F Dissociation of FAK/p130(CAS)/c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK J Cell Biol 1999. 144:315-24

» Owen, J D, Ruest, P J, Fry, D W, Hanks, S K Induced focal adhesion kinase (FAK) expression in FAK-null cells enhances cell spreading and migration requiring both auto- and activation loop phosphorylation sites and inhibits adhesion-dependent tyrosine phosphorylation of Pyk2 Mol Cell Biol 1999. 19:4806-18

» Zhang, X, Chattopadhyay, A, Ji, Q S, Owen, J D, Ruest, P J, Carpenter, G, Hanks, S K Focal adhesion kinase promotes phospholipase C-gamma1 activity Proc Natl Acad Sci U S A 1999. 96:9021-6

» Cary, L A, Han, D C, Polte, T R, Hanks, S K, Guan, J L Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration J Cell Biol 1998. 140:211-21

» Hanks, S K, Polte, T R Signaling through focal adhesion kinase Bioessays 1997. 19:137-45

» Polte, T R, Hanks, S K Complexes of focal adhesion kinase (FAK) and Crk-associated substrate (p130(Cas)) are elevated in cytoskeleton-associated fractions following adhesion and Src transformation. Requirements for Src kinase activity and FAK proline-rich motifs J Biol Chem 1997. 272:5501-9

» Withers, B E, Hanks, S K, Fry, D W Correlations between the expression, phosphotyrosine content and enzymatic activity of focal adhesion kinase, pp125FAK, in tumor and nontransformed cells Cancer Biochem Biophys 1996. 15:127-39

» Calalb, M B, Zhang, X, Polte, T R, Hanks, S K Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src Biochem Biophys Res Commun 1996. 228:662-8

» Polte, T R, Hanks, S K Interaction between focal adhesion kinase and Crk-associated tyrosine kinase substrate p130Cas Proc Natl Acad Sci U S A 1995. 92:10678-82

» Zhang, X, Wright, C V, Hanks, S K Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development Gene 1995. 160:219-22

» Calalb, M B, Polte, T R, Hanks, S K Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases Mol Cell Biol 1995. 15:954-63

» Hanks, S K, Hunter, T Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification FASEB J 1995. 9:576-96

» Kitagawa, M, Higashi, H, Suzuki-Takahashi, I, Segawa, K, Hanks, S K, Taya, Y, Nishimura, S, Okuyama, A Phosphorylation of E2F-1 by cyclin A-cdk2 Oncogene 1995. 10:229-36

» Gates, R E, King, L E, Hanks, S K, Nanney, L B Potential role for focal adhesion kinase in migrating and proliferating keratinocytes near epidermal wounds and in culture Cell Growth Differ 1994. 5:891-9

» Schlaepfer, D D, Hanks, S K, Hunter, T, van der Geer, P Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase Nature 1994. 372:786-91

» Polte, T R, Naftilan, A J, Hanks, S K Focal adhesion kinase is abundant in developing blood vessels and elevation of its phosphotyrosine content in vascular smooth muscle cells is a rapid response to angiotensin II J Cell Biochem 1994. 55:106-19

» Devor, B B, Zhang, X, Patel, S K, Polte, T R, Hanks, S K Chicken and mouse focal adhesion kinases are similar in structure at their amino termini Biochem Biophys Res Commun 1993. 190:1084-9

» Gates, R E, Hanks, S K, King, L E Focal-adhesion components are enriched in ventral membranes isolated from transformed keratinocytes in culture Biochem J 1993. 289 ( Pt 1):221-6

» Hanks, S K, Calalb, M B, Harper, M C, Patel, S K Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin Proc Natl Acad Sci U S A 1992. 89:8487-91

» Calalb, M B, Fox, D T, Hanks, S K Molecular cloning and enzymatic analysis of the rat homolog of 'PhK-gamma T,' an isoform of phosphorylase kinase catalytic subunit J Biol Chem 1992. 267:1455-63

» Matsushime, H, Ewen, M E, Strom, D K, Kato, J Y, Hanks, S K, Roussel, M F, Sherr, C J Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins Cell 1992. 71:323-34

» Hanks, S K, Lindberg, R A Use of degenerate oligonucleotide probes to identify clones that encode protein kinases Methods Enzymol 1991. 200:525-32

» Hanks, S K, Quinn, A M Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members Methods Enzymol 1991. 200:38-62

» Hanks, S.K Eukaryotic protein kinases Curr. Op. Struct. Biol. 1991. 1:369-383

» Lawton, M A, Yamamoto, R T, Hanks, S K, Lamb, C J Molecular cloning of plant transcripts encoding protein kinase homologs Proc Natl Acad Sci U S A 1989. 86:3140-4

» Hanks, S K Messenger ribonucleic acid encoding an apparent isoform of phosphorylase kinase catalytic subunit is abundant in the adult testis Mol Endocrinol 1989. 3:110-6

» Maldonado, F, Hanks, S K A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha Nucleic Acids Res 1988. 16:8189-90

» Hanks, S K, Quinn, A M, Hunter, T The protein kinase family: conserved features and deduced phylogeny of the catalytic domains Science 1988. 241:42-52

» Hanks, S K, Armour, R, Baldwin, J H, Maldonado, F, Spiess, J, Holley, R W Amino acid sequence of the BSC-1 cell growth inhibitor (polyergin) deduced from the nucleotide sequence of the cDNA Proc Natl Acad Sci U S A 1988. 85:79-82

» Hanks, S K Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family Proc Natl Acad Sci U S A 1987. 84:388-92

» Mitchell, R L, Hanks, S K, Verma, I M Proto-oncogene fos: an inducible multifaceted gene Symp Fundam Cancer Res 1986. 39:99-113

» Hanks, S K, Riggs, M G Selective insolubility of active hsp70 gene chromatin Biochim Biophys Acta 1986. 867:124-34

» Brown, D B, Hanks, S K, Murphy, E C, Rao, P N Early initiation of DNA synthesis in G1 phase HeLa cells following fusion with red cell ghosts loaded with S-phase cell extracts Exp Cell Res 1985. 156:251-9

» Gollin, S M, Wray, W, Hanks, S K, Hittelman, W N, Rao, P N The ultrastructural organization of prematurely condensed chromosomes J Cell Sci Suppl 1984. 1:203-21

» Hanks, S K, Gollin, S M, Rao, P N, Wray, W, Hittelman, W N Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes Chromosoma 1983. 88:333-42

» Hanks, S K, Rodriguez, L V, Rao, P N Relationship between histone phosphorylation and premature chromosome condensation Exp Cell Res 1983. 148:293-302

» Hanks, S K, Brown, D B, Rao, P N Induction of premature chromosome condensation at high frequency following polyethylene glycol-mediated fusion of lectin-bound cells Exp Cell Res 1982. 138:215-9

» Rao, P N, Hanks, S K Chromatin structure during the prereplicative phases in the life cycle of mammalian cells Cell Biophys 1980. 2:327-37

» Hanks, S K, Rao, P N Initiation of DNA synthesis in the prematurely condensed chromosomes of G1 cells J Cell Biol 1980. 87:285-91