The Vanderbilt Institute for Clinical and Translational Clinical Research Center Laboratory, a CAP/CLIA certified laboratory is available for your research assay testing needs. A diverse team of technologists and instrumentation specialists with over fifty years of combined experience are here to assist you. The following analyses are currently available.
HPLC and ELISA Laboratory
DNA Extraction (B)
Catecholamine (P, U)
Total Nitrogen (U)
* B = Blood, P = Plasma, S = Serum, Sal = Saliva, U = Urine
DNA extraction is performed manually, by licensed technologists using Qiagen Puregene DNA purification. This provides archive-quality DNA for your long-term storage.
Our Catecholamine analysis, using the latest High Performance Liquid Chromatography (HPLC) instrumentation, adhering to strict National Institute of Health (NIH) standards, is second to none world-wide with separation, identification, purification, and quantification. The laboratory currently offers Catecholamine analysis, which includes epinephrine (E), norepinephrine (NE), dihydroxyphenylglycol (DHPG), dihydroxyphenyl-acetic acid (DOPAC), dihydroxyphenyl-L-alanine (DOPA), and dopamine hydrochloride (DA).
State of the art ELISA assays are provided by the lab. We work hand-in-hand with commercial vendors to provide a collaborative relationship with our customers. The 96 well kits, combined with our innovative instrumentation provide a symbiotic relationship with the investigators. This results in a diverse line of assays that appeal to a limitless provider need base. In other words, if the kit is offered commercially by a reputable dealer, fits our instrumentation parameters, we will work with you in an attempt to accurately analyze and technically support your ELISA testing needs.
Patient Sample Submission for inpatients is completed by the nursing staff. Outpatient samples are scheduled and requisitioned with either nursing staff or with the technologists in the Laboratory, depending on the testing services needed. Sample storage after testing is for three years, according to College of American Pathologists (CAP) regulations. After that, the investigator is responsible for his or her sample storage. All sample storage must be arranged with technologists prior to testing after VICTR approval.
Sample result retrieval is accomplished using the Vanderbilt VUnet ID access code to access the CRC result database. This secure website data is only available to key personnel approved by the VICTR approved investigator.
The VICTR CRC Clinical Research Laboratory staff looks forward to assisting you with your laboratory testing needs.
Vanderbilt Clinical Research Center Core Lab
1161 21st Ave. South
Nashville, TN 37232-2195
The Catecholamine procedure performed in the CRC Core laboratory for plasma and urine is an extraction technique using alumina. The quantitation of catecholamines is accomplished employing the HPLC method, high performance liquid chromatography with electrochemical detection. In the initial step of the procedure, compounds are separated from one another based on relative strength of their interaction with a stationary phase (a C18 hydrocarbon chain bonded to a 5um silica particle packed into a column) and a mobile phase. The mobile phase is a buffer pumped through a column. The two phases compete for the sample molecules attraction. The sample molecules with the greater attraction to the mobile phase will elute first, therefore will have the shorter retention time. The sample molecules with the most attraction to the stationary front will elute last and have a longer retention time.
In the second part of the HPLC method, the eluate after passing through the column passes through a series of electrochemical cells. The sample molecules are first oxidized at the conditioning cell. At the first analytical electrode a screening reaction takes place to eliminate interference but the catecholamines do not react. At the second analytical cell, reduction of the catecholamines is accomplished and the observed voltage is converted to a chromatogram. On the chromatogram peaks are observed representing the six catecholamines in each sample analyzed. The height of each peak is directly proportional to the amount of catecholamine analyte in the sample.