pri-miRNA nuclear processing: Drosha
Figure 2. Mating Strategy.  Excerpted from "Rescue of Pituitary Function in a Mouse Model of Isolated Growth Hormone Deficiency Type II by RNAi" by N. Shariat et al

Figure 5.  The TDRD7 3′UTR is targeted by miR-430 in somatic cells and allows protein expression in PGCs. Excerpted from "Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430" by Y. Mishima et al.

Figure 2. The mRNA Cycle: A Model for the Cycling of Eukaryotic mRNAs between Different Subcellular Compartments.  Excerpetd from P bodies and the control of mRNA translation and degradation by A. Eulalio et al.
Tissue specific miRNA expression: in-situ expression


 

The The Module 1 Exam is now available
All exams are DUE to Kim Kane by February 14, 2008
Christopher Wright (Organizer and Instructor )
Professor - Cell and Developmental Biology 

Guoqiang Gu (Organizer and Instructor) 
Assistant Professor - Cell and Developmental Biology

Jim Patton (Instructor)
 Professor - Biological Sciences
(For more information about CBIO341 please contact the Course Coordinator)

There are two books for this course that are highly recommended:  Principles of Development by Lewis Wolpert, and Developmental Biology by Scott F. Gilbert.  Students should also familiarize themselves with the basic concepts of embryology by reviewing the following background information:

Introduction to Embryology  (Background Lectures & Readings)
A broad-based overview-type group of lectures and readings designed to bring students up to speed and optimize engagement and learning for the 2008 modules. The goal is to teach essential terms and concepts of embryology so that students can move rapidly from classical descriptive and experimental embryology to recent findings that have high impact at the molecular and mechanistic levels.  Topics covered by these presentations include: key concepts, terms and definitions, frog, mouse, and zebrafish early development including comparative studies of gastrulation, fruitfly development, and ectodermal, mesodermal, and endodermal derivatives

Click on the appropriate module name for class specifics by date.

Class Date
Topics, Presentations & Readings
1/15/08 microRNA Fundamentals - Part I  (Wright)
1/17/08
 "The Role of microRNA's during Zebrafish Development"   Antonio Giraldez, Ph.D. 
BACKGROUND READING
"Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430" by W-Y Choi et al
1/22/08 microRNA Fundamentals - Part II  (Wright)
       DOI LINKS:  BACKGROUND READING FOR microRNA FUNDAMENTALS
1/24/08 Personal Lab Description of microRNA Research (Patton)
1/29/08 Bioinformatics, Target Selection, Loss of Function Methods and Applications (Gu)
1/31/08
Loss of Function - Dicer, Individual microRNAs, Animals (cell lines, flies, and mice)
2/5/08 Ago/Piwi in Development (Gu)   Student groups notify Sarah Kucenas of 2/7 paper choices
2/7/08 Postdoctoral Fellow/Grad Student led discussion of student-chosen microRNA papers (Kucenas, Mundell, Jernigan )
   
  GROUP 1 READINGS
  Nathan Mundell (Leader), Valda Hilliard, Jeff Bennett, Jenny Plank, Tocarra Cecil, Mallory Hacker, Spencer Willet, and David Shifrin
READING 1 Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA. 
2007 J. Biol. Chem 282: 17649-17657
READING 2 Processing of pre-microRNAs by the Dicer-1 Loquacious complex in Drosophila cells.
2005 PLos Biol 3:e235
   
  GROUP 2 READINGS
  Sarah Kucnas (Leader), Abby Olena, Jonathan Fleming, Billy Carver, Chunyao Wei, Jessica Mazerik, Tomas You,and David Melville
READING 1 Argonaute 1 regulates the fate of germline stem cells in Drosophila. 
2007 Development 134: 4265-4272
READING 2 RNA Pol IV plays catch with Argonaute 4. 
2007 Cell 131: 643-645
   
  GROUP 3 READINGS
  Kristin Jernigan (Leader), Robert Smith, Jiang Liu, Ben Grady, Yinzi Liu, Rachel Reinert, and JoAnn Cooper
READING 1 P bodies and the control of mRNA translation and degradation.
2007 Mol Cell 25: 635-646
READING 2 P-body formation is a consequence, not the cause, of RNA-mediated gene silencing.
2007 Mol Biol Cell 27: 3970-3981
 
The Module 1 Exam will be distributed on February 7, 2008 and is
DUE by February 14, 2008
 

 

  

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Copyright 2004, Educational Technology, Biomedical Research Education & Training
 Last modified: Monday, February 4, 2008 by Kim.Kane@vanderbilt.edu