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Days 7 through 9 of Boot Camp focus on the classic contributions of Mus musculus, or the common house mouse, to developmental biology research: 1) the derivation of embryonic stem (ES) cells and homologous recombination in ES cells to manipulate gene expression, 2) transgenic manipulation of gene expression, and 3) models for human disease.
Note that these lectures will be held on Tuesday (6/23), Wednesday (6/24) and Tuesday (6/30)
Before your training begins on Tuesday, June 23, you should read the following:
|
Gilbert's 7th Edition "Developmental
Biology" - Chapter |
|
| Wolpert's 2nd Edition "Principles of Development" | |
| The Mouse (p 41-46) | |
| Mouse Axes (p 77-80) | |
| Mouse Neural Development (p 278-280) | |
| Mouse Neural Crest (p 313-315) | |
| Torrey's 2nd Edition "Morphogenesis of the Vertebrates" | |
| Chapter 8 Amphibian Cleavage and Germ Layer Formation (p 83-90) | |
|
TRAINING
DAY 7 - PRESENTATION
(not
yet available) |
|
| AIM
1 To compare cleavage and blastocyst formation with other vertebrate and invertebrate animals |
|
|
AIM 2
To understand how gastrulation gives rise to both embryonic and extra-embryonic structures |
|
|
AIM 3
To discuss mechanisms of gastrulation; contrast primitive streak, node and movement of gastrulating cells in the mouse with chick, frog and fish development |
|
|
AIM 4
To discuss germ layer formation and subsequent derivatives |
|
| AIM
5 To understand how the neural tube becomes patterned along dorsal ventral axis |
|
| AIM
6 To discuss formation of the neural crest cell population and its subsequent derivatives |
|
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PROS & CONS OF
USING MUS MUSCULUS
|
|
 |
Early development of the mouse embryo more closely relates to human development |
|
|
Mammals are the only model organisms relevant to study placentation |
 |
It is difficult to perform classical 'cut and paste' type of experiments |
|
|
Development is internal and thus embryos cannot be easily observed over time, although methods for embryo culture do exist |
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Mice are amenable to both forward and reverse genetics to analyze genes involved in developmental processes |
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Lineage tracing studies with cell marking techniques or genetic lineage labels can be performed |
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University is committed to principles of equal opportunity and affirmative
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Copyright 2004, Educational
Technology, Biomedical Research Education & Training
Last modified: Monday, June 22, 2009 by
Kim.Kane@vanderbilt.edu