Figure 6: Behaviour screens: visual adaptation mutants.  Excerpted From "The art and design of genetic screens: zebrafish"
BOOT CAMP ORGANISMS
What Is Developmental Biology?
Roadmap to the Embryo
Early Fish Development
Neural Fish Development
Mouse
Chick
Worm
Fly
Frog
Trainee Contributions
 
BOOT CAMP DIRECTORS
David Bader  Director
Hillary Hager Student Director
Rachel Skelton Student Director in Training
Kim Kane Support
 
BOOT CAMP INSTRUCTORS
David Bader   Basics
Josh Gamse  Fish
Maureen Gannon   Mouse
Hillary Hager  Chick
Trish Labosky  Mouse
Laura Lee  Fly
David Miller  Worm
Chris Wright  Frog


Day 6 of Boot Camp continues to focus on the classic contributions of Danio rerio, or the zebrafish to developmental biology research: 1) forward genetic screens, and 2) early development mutants in a vertebrate model.

Before your training begins on Thursday, June 18, you should read the following:

TRAINING DAY 6 - PRESENTATION (available)
AIM 1
To appreciate the power of forward genetics in understanding vertebrate development
AIM 2
To examine zebrafish neurulation: how fish "roll their own" compared to other vertebrates
AIM 3
To investigate the progressive refinement of the anteroposterior pattern of the nervous system from gastrulation onwards
AIM 4
To understand that the organizers in the nervous system are not just for gastrulation anymore
AIM 5
Making a case for nervous system development - how a clear brain and fast development can reveal a lot
 
PROS & CONS OF USING ZEBRAFISH IN NEURAL RESEARCH
Live imaging of developing zebrafish brains plus transgenes has allowed detailed studies of cells in vivo
yes
 The use of fluorescent photo-convertible and lipophilic compounds permits straightforward lineage labeling and axon tracing
Explant embryology is very difficult compared to chick and frog
no
 Conditional expression is in its infancy so conditional mutations are not yet possible
yes
 Forward genetics has identified mutants affecting CNS development, sometimes surprising in gene identity
 

 

  

Vanderbilt University is committed to principles of equal opportunity and affirmative action

Copyright 2004, Educational Technology, Biomedical Research Education & Training
 Last modified: Monday, June 22, 2009 by Kim.Kane@vanderbilt.edu