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Day 5 of Boot Camp focuses on understanding the classic contributions of Danio rerio, or the zebrafish to developmental biology research: 1) fate restriction and germ layer formation, and 2) In vivo imaging of various developmental processes.
Before your training begins on Tuesday, June 16, you should read the following:
- Gilbert's 7th Edition "Developmental Biology" - Chapter 11 (pgs 341-381)
| TRAINING DAY 5 - PRESENTATION (available) | |
| EARLY ZEBRAFISH DEVELOPMENT MOVIES | |
| In Toto Microscopy | |
| In Toto Reconstruction | |
| Animal Hemisphere Movements | |
| Animal Hemisphere Cell Divisions | |
| Dorsal Hemisphere Microscopy | |
| AIM
1 To understand the pattern of zebrafish cleavage compared to that seen in birds, mammals, reptiles and amphibians |
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AIM 2
To be able to compare and contrast the cellular mechanisms of zebrafish epiboly and gastrulation |
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AIM 3
To understand germ layer formation and compare to other species |
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AIM 4
To be able to integrate fate mapping with experimental evidence for signaling centers as a way to understand pattern formation. |
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PROS & CONS OF USING ZEBRAFISH
IN RESEARCH
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The zebrafish embryo is outstanding for fate mapping, in vivo imaging, gene overexpression studies and forward genetics |
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Transgenesis is good but lacks the sophistication of mouse tools |
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Various reverse genetics approaches are available but have limitations |
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Useful homologous recombination has not been reported |
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The genome is being sequenced and ESTs are available |
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Copyright 2004, Educational
Technology, Biomedical Research Education & Training
Last modified: Monday, June 22, 2009 by
Kim.Kane@vanderbilt.edu