- In Vivo Metabolic
Regulation and Obesity - Islet Development,
Biology, and
Immunology - Complications of
Diabetes - Oxidative stress
- Signal transduction
and Gene Regulation - Clinical Research
- Translational Research
November 23, 2009
Summary of Services & Methods –
Catecholamines
Plasma norepinephrine and epinephrine are measured by HPLC via electrochemical detection using the method adapted from Anton and Sayre (1962). 250 micro liters of plasma are absorbed onto alumina at a ph of 8.6, eluted with dilute perchloric acid, and auto-injected onto a c18 reversed-phase column. An internal standard (dehydroxylbenzylamine / DHBA) is included with each extraction to monitor recovery and aid in quantitation. Results are quantitated through a chromatography data station.
