- In Vivo Metabolic
Regulation and Obesity - Islet Development,
Biology, and
Immunology - Complications of
Diabetes - Oxidative stress
- Signal transduction
and Gene Regulation - Clinical Research
- Translational Research
November 23, 2009
Analysis of Isoprostanes
A highly sensitive and specific method for the analysis of isoprotanes in vitro and in vivo is utilized. The methodology is based primarily upon capillary column gas chromatography (GC) negative ion chemical ionization (NICI)mass spectrometry (MS) or liquid chromatography (LC)/MS using stable isotope dilution techniques employing deuterated or [18O]-labeled internal standards. The assays offered are parent F2-isoprostanes and the major urinary isoprostane metabolite (2,3-dinor-5,6,-dihydro-15-F2t-IsoP,F2-isoprostane-M).
