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December 03, 2008

Radioimmunoassay / Endocrine Assays

Insulin, Glucagon, Leptin, Growth Hormone, C-peptide, Cortisol, Salivary Cortisol, IGF-1, Ghrelin, Pancreatic Polypeptide, Aldosterone, ACTH, etc.

All hormones are assayed via RIA using a double antibody procedure. Radioimmunoassay allows for the detection of minute concentrations of biological (hormones) or pharmacological substances in blood or other fluid samples using antigen/antibody reactions. The method provides a very sensitive, inexpensive method of measurement. Final analysis is accomplished through quantifying the bound radioactive counts with a Packard Gamma counter connected to a computerized data reduction station.

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The Vanderbilt Diabetes Research and Training Center is supported by NIH grant DK20593.

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