May 23, 2013
Radioimmunoassay / Endocrine Assays
Insulin, Glucagon, Leptin, Growth Hormone, C-peptide, Cortisol, Salivary Cortisol, IGF-1, Ghrelin, Pancreatic Polypeptide, Aldosterone, ACTH, etc.
All hormones are assayed via RIA using a double antibody procedure. Radioimmunoassay allows for the detection of minute concentrations of biological (hormones) or pharmacological substances in blood or other fluid samples using antigen/antibody reactions. The method provides a very sensitive, inexpensive method of measurement. Final analysis is accomplished through quantifying the bound radioactive counts with a Packard Gamma counter connected to a computerized data reduction station.
The Vanderbilt Diabetes Research and Training Center is supported by NIH grant DK20593.
