For more data about these procedures, contact David Wasserman, Ph.D.
Core personnel have the experience required to perform numerous, quantitatively precise experimental techniques
that are unique to this laboratory. These are outlined below:
- Glucose clamp technique (rat, dog): This procedure allows the circulating blood glucose concentration
to be held at a desired level. This can be achieved during intravenous insulin infusions or
in situations which require constant glucose levels.
- Amino acid clamp technique (rat, dog): This very similar to the glucose clamp technique
and permits precise regulation of circulating concentrations of individual amino acids.
Currently the technique is limited to only a few amino acids, primarily the branch chained amino acids
The technique is dependent upon the ability to monitor the concentrations of the amino acids rapidly
(less than 10 min.).
- Pancreatic clamp technique (rat, dog): This is accomplished by the introduction of somatostatin into a peripheral vein
at a concentration that inhibits the endogenous release of insulin and glucagon and at the same time
an intraportal infusion of insulin and glucagon is initiated.
- Indirect calorimetry (rat, dog): The laboratory has developed surgical techniques and invested
in equipment that allows investigators to address the rates of whole body substrate oxidation by the assessment
of VO2 and VCO2 rates in the expired air.
In large animals this is accomplished with the introduction of a soft tracheostomy cuff
into a previously constructed permanent tracheostomy at the time of the experiment.
In small animals whole body VO2 and VCO2 are measured continuously using an Oxymax Deluxe System.
Both large animal and small animal systems consist of a personal computer based measurement system
that employs the host computer as both a system controller and as a data collection, storage, and presentation device.
- Measurement of blood pressure (rat, dog): Blood pressure represents a remarkably integrated measure
of overall cardiovascular function and is affected by stroke volume, heart rate, inotropic state, and vascular tone.
Abnormalities of blood pressure regulation (primarily hypertension, but also hypotension) are associated
with major cardiovascular morbidity and mortality and are epidemiologically associated with diabetes.
Blood pressure can be measured in large and small animals with a transducer connected to an indwelling arterial catheter.
Additionally, in small animals, measurements of blood pressure can be made noninvasively using a tail cuff.