Models of Diabetes and Metabolic Disorders
For more data about these procedures, contact David Wasserman, Ph.D..
The laboratory makes available animal models created by various surgical and/or experimental techniques.
The models simulate or produce various disease states or physiologic states.
In the provision of these models the personnel of the core provide the daily care and management of the animal
and report to the investigators on the condition of the animals.
Again many of the experimental models described below can be adapted for the pig.
- Chronic diabetic model (rat, dog): This model provides investigators with animals that are insulin deficient.
Two approaches have been utilized in the past in large animals, pancreatectomy and chemical induction.
In addition, Dr. Cherrington has worked with the core to establish a model of T2DM in the dog,
which should have considerable utility.
- Adrenalectomized model (rat, dog): This model provides the investigators with an animal devoid of cortisol
and epinephrine and allows the investigator to manipulate replacement therapy or address questions relating
to catecholamine and/or glucocorticoid action, hypoglycemia or stress.
- Hypophysectomy model (rat): Hypophysectomies are performed in animals to induce pituitary insufficiency.
This procedure permits investigators to examine the role of pituitary hormones such as
growth hormone, prolactin, thyrotropin etc.
- Exercise models (rat, dog): These models provide investigators with animals that are acclimated to a treadmill.
For large animals a treadmill was modified to accommodate exercising dogs while allowing access to catheters.
For small animals two methods of assessment are possible.
Each method permits investigators to address specific questions such as spontaneous exercise activity
and treadmill exercise to study the response to acute muscular work.
- Denervated liver model (dog): This model provides investigators with a conscious animal that has no innervation
to the liver, but otherwise healthy.
- Denervated pancreas model (dog): The laboratory has developed a surgical denervation model that permits investigators
to examine the neural control of the pancreas.
- Vagal nerve impairment (dog): This model allows investigators to address the role
of the parasympathetic nervous system in modulating hepatic metabolism.