Recent Publications


McDonald NA, Vander Kooi CW, Ohi MD, Gould KL. (2015). Oligomerization but not membrane bending underlies the function of certain F-BAR proteins in cell motility and cytokinesis. Dev Cell In press

Makarova M, Gu Y, Chen JS, Beckley JR, Gould KL, Oliferenko S. (2015). Temporal regulation of Lipin activity diverged to account for differences in mitotic programs. Curr Biol In press

Ullal P, McDonald NA, Chen JS, Lo Presti L, Roberts-Galbraith RH, Gould KL, Martin SG. (2015). The DYRK-family kinase Pom1 phosporylates the F-BAR protein Cdc15 to prevent division at cell poles. J Cell Biol 211(3):653-68

McDonald NA, Gould KL. (2015). Characterization of cytokinetic F-BARs and other membrane binding proteins. Methods Mol Biol 1369:181-9

Beckley JR, Chen JS, Yang Y, Peng J, Gould KL. (2015). A degerate cohort of yeast membrane trafficking DUBs mediate cell polarity and survival. Mol Cell Proteomics In press

Willet AH*, McDonald NA*, Gould KL. (2015). Regulation of contractile ring formation and septation in Schizosaccharomyces pombe. Curr Opin Microbiol 28:46-52

Willet AH, McDonald NA, Bohnert KA, Baird MA, Allen JR, Davidson MW, Gould KL. (2015). The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12. J Cell Biol 208(4):391-9

Chen JS, Beckley JR, McDonald NA, Ren L, Mangione M, Jang SJ, Elmore ZC, Rachfall N, Feoktistova A, Jones CM, Willet AH, Guillen R, Bitton DA, Bähler J, Jensen MA, Rhind N, Gould KL. (2015). Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains. Genes, Genomes, Genetics 5(3):361-70

Ren L, Willet AH, Roberts-Galbraith RH, McDonald NA, Feoktistova A, Chen JS, Huang H, Guillen R, Boone C, Sidhu SS, Beckley JR, Gould KL. (2015). The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast. Mol Biol Cell 26(2):256-69.


Elmore ZC, Beckley JR, Chen JS, Gould KL. (2014). Histone H2B Ubiquitination Promotes the Function of the Anaphase-Promoting Complex/Cyclosome in Schizosaccharomyces pombe. Genes, Genomes, Genetics 4(8):1529-38.

Ma H, McLean JR, Gould KL, McCollum D. (2014). An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells. Methods Mol Biol 1177:175-91.

Rachfall N, Johnson AE, Mehta S, Chen JS, Gould KL. (2014). Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network. Mol Biol Cell 5(15):2250-9.


Bohnert KA, Willet AH, Kovar DR, Gould KL. (2013). Formin-based control of the actin cytoskeleton during cytokinesis. Biochem Soc Trans. 41(6):1750-4.

Johnson AE, Chen JS, Gould KL. (2013). CKI is required for a mitotic checkpoint that delays cytokinesis. Current Biology. 23:1920-1926.

Bohnert KA, Grzegorzewska AP, Willet AH, Vander Kooi CW, Kovar DR, Gould KL. (2013). SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis. Genes & Development. 27:2164-2177

Bajpai A, Feoktistova A, Chen JS, McCollum D, Sato M, Carazo-Salas RE, Gould KL, Csikász-Nagy A. (2013). Dynamics of SIN Asymmetry Establishment. PLoS Comput Biol. 9(7):e1003147.

Dephoure N, Gould KL, Gygi SP, Kellogg DR. (2013).Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Mol Biol Cell. 24(5):535-42.

Gupta S, Mana-Capelli S, McLean JR, Chen CT, Ray S, Gould KL, McCollum D. (2013). Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Current Biology. 23(4):333-8.

Chen JS, Broadus MR, McLean JR, Feoktistova A, Ren L, Gould KL. (2013). Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Mol Cell Proteomics. 12(5):1074-86.


Gupta S, Mana-Capelli S, McLean JR, Chen CT, Ray S, Gould KL, McCollum D. (2012). Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Current Biology. 23(4):333-8.

Mana-Capelli S, McLean JR, Chen CT, Gould KL, McCollum D. (2012). The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning. Mol Biol Cell. 23(23):4592-600.

Clément A, Solnica-Krezel L, Gould KL. (2012). Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Dev Dyn. 241(12):1911-21.

Broadus MR and Gould KL. (2012). Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress. Mol Biol Cell. 23(20):4118-28.

Bohnert KA and Gould KL. (2012). Cytokinesis-based constraints on polarized cell growth in fission yeast. PLoS Genetics. 8(10):e1003004.

Wang Y, Li WZ, Johnson AE, Luo ZQ, Sun XL, Feoktistova A, McDonald WH, McLeod I, Yates JR III, Gould KL, McCollum D, and Jin QW. (2012). Dnt1 Acts as a mitotic inhibitor of the spindle checkpoint protein Dma1 in fission yeast Mol Biol Cell. 23(17):3348-56.

Johnson AE, McCollum D, Gould KL. (2012). Polar opposites: Fine-tuning cytokinesis through SIN asymmetry. Cytoskeleton. 69(10):686-99.

Johnson AE, Collier SE, Ohi MD, Gould KL. (2012). Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function. J Biol Chem. 287(31):25741-8.

Lu LX, Domingo-Sananes MR, Huzarska M, Novak B, Gould KL. (2012). Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches. Proc Natl Acad Sci. 109(25):9899-904. 

Pancaldi V, Sarac O.S, Převorovský M, McLean JR, Rallis C, Gould KL, Beyer A, and Bähler J. (2012). Predicting the fission yeast protein interaction network. G3: Genes, Genomes and Genetics. 2(4):453-467. 

Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D. (2012). A highly efficient multifunctional tandem affinity purification approachapplicable to diverse organisms. Mol Cell Proteomics. 11(8):501-511.

Feoktistova A, Morrell-Falvey J, Chen JS, Singh NS, Balasubramanian MK, Gould KL. (2012). The fission yeast SIN kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11. Mol Biol Cell. 23(9):1636-45.


Singh NS, Shao N, McLean JR, Sevugan M, Ren L, Chew TG, Bimbo A, Sharma R, Tang X, Gould KL, Balasubramanian MK. (2011). SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast. Current Biology. 21(23):1968-78.

Chen JS, Lu LX, Ohi MD, Creamer KM, English C, Partridge JF, Ohi R, Gould KL. (2011). Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 195(4):583-93.

Převorovský M, Atkinson SR, Ptáčková M, McLean JR, Gould KL, Folk P, Půta F, Bähler J. (2011). N-termini of fungal CSL transcription factors are disordered, enriched in regulatory motifs and inhibit DNA binding in fission yeast. PLoS One. 6(8):e23650.

Peckys DB, Mazur P, Gould KL, de Jonge N. (2011). Fully hydrated yeast cells imaged with electron microscopy. Biophys J. 100(10):2522-9.

Bohnert KA, Gould KL. (2011). On the cutting edge: post-translational modifications in cytokinesis. Trends Cell Biol. 21(5):283-92.

McLean JR, Chaix D, Ohi MD, Gould KL. (2011). State of the APC/C: organization, function, and structure. Crit Rev Biochem Mol Biol. 46(2):118-36

Almonacid M, Celton-Morizur S, Jakubowski JL, Dingli F, Loew D, Mayeux A, Chen JS, Gould KL, Clifford DM, Paoletti A. (2011). Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin. Current Biology. 21(6):473-9

McLean JR, Kouranti I, Gould KL. (2011). Survey of the phosphorylation status of the Schizosaccharomyces pombe deubiquitinating enzyme (DUB) family. J Proteome Res. 10(3):1208-15.

Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, Ohi MD, Gould KL. (2011). Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS One. 6(2):e16719.

Goettel JA, Liang D, Hilliard VC, Edelblum KL, Broadus MR, Gould KL, Hanks SK, Polk DB. (2011). KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1. Exp Cell Res. 317(4):452-63

Johnson AE, Gould KL. (2011). Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.
EMBO. 30(2):341-54

Clément A, Solnica-Krezel L, Gould KL. (2011). The Cdc14B phosphatase contributes to ciliogenesis in zebrafish.
. 138(2):291-302.


Papadopoulou K, Chen JS, Mead E, Feoktistova A, Petit C, Agarwal M, Jamal M, Malik A, Spanos A, Sedgwick SG, Karagiannis J, Balasubramanian MK, Gould KL, McInerny CJ. (2010). Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p. J Cell Sci. 123:4374-81.

Roberts-Galbraith RH and Gould KL. (2010). Setting the F-BAR: functions and regulation of the F-BAR protein family.
Cell Cycle. 9(20):4091-7

Kouranti I, McLean JR, Feoktistova A, Liang P, Johnson AE, Roberts-Galbraith RH, Gould KL. (2010). A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity. PLoS Biology. 8(9):e1000471.

Roberts-Galbraith RH, Ohi MD, Ballif BA, Chen JS, McLeod I, McDonald WH, Gygi SP, Yates JR, Gould KL. (2010). Dephosphorylation of F-BAR Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.
Molecular Cell 39(1):86-99.

Vander Kooi CW, Ren L, Xu P, Oh, MD, Gould KL, Chazin WJ. (2010) The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure. 18(5):584-593.


Bohnert KA, Chen JS, Clifford DM, Vander Kooi CW, Gould KL. (2009) A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein. Mol Biol Cell. 20(16):3646-59.

Roberts-Galbraith RH, Chen JS, Wang J, Gould KL. (2009) The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring. J Cell Biol. 184(1): 113-127.