The overall goal of the Histology and Morphometry Core is 1) to provide a common facility and expertise for the broad spectrum of morphologic studies that may be required for interpretation of studies of various models of kidney disease and their response to interventions; 2) to provide tissue samples from existing archival specimens from various mouse and rat kidney disease models that may be used for exploration and characterization of expression patterns of various novel target molecules; and 3) to develop and enhance existing morphologic techniques to improve the ability of center investigators to localize specific molecules and tissue sections, and to interpret their significance based on the spatial localization and relationship to patterns of injury. The Core will also maintain a dedicated, upto date and interactive renal immunohistochemistry database that will outline all of the availlable renal cell specific antibodies, their characteristics and associated staining protocols.
The specific objectives are as follows:
1) To provide expert consultation to all investigators on procedures for harvesting and processing of tissues and preparation of these prior to submission to the Core for analysis.
2) To provide appropriate processing of tissue, including freezing, fixation in various media, paraffin-embedding, and sectioning.
3) To provide routine H&E and special stains as needed, including PAS, Jones' Silver Stain, Elastic and Masson Trichrome stains.
4) To provide immunohistochemistry for selected panels of tested and validated antibodies.
5) To provide expert advice on harvesting and selection of tissue for electron microscopic studies, including selection of appropriate portions of tissue from thick scout sections for subsequent electron microscopic thin sectioning and examination.
6) To provide expert advice and services for basic morphometry of key aspects of renal structure, including glomerular size, mesangial expansion, tubulointerstitial fibrosis, and GBM thickness (EM).
7) To provide archival tissue samples from tissue arrays of various disease phenotypes for investigators' use in pilot studies to detect key novel molecules of interest, and to determine if they are affected by manipulations with proven effects in our archival tissue samples.
8) To provide expert analysis of material submitted to the Core for processing of appropriately prepared outside material, including detailed structural analysis of structural injury affecting the glomerulus, tubulointerstitium and vessels.