February 22, 2011

The Department of Microbiology and Immunology Research in Progress Seminar Series, Thursday, Feb.24, 9:30 a.m.

Michelle LaFrance, Graduate Student
From the Laboratory of Borden Lacy, Ph.D.


“Host Factors Involved in Clostridium difficile TcdB Cytotoxicity”


Clostridium difficile has become a major public health problem in that it causes hospital-acquired diarrheal cases throughout the United States. Virulence of C. difficile is associated with expression of two homologous Large Clostridial Toxins, TcdA and TcdB. These toxins enter host cells and monoglucosylate members of the Rho and Ras families of GTPases, but, in fact, many of the cellular factors involved in cytotoxicity remain unknown.

We have used insertional “gene-trap” mutagenesis to identify mutations in human intestinal epithelial cells that confer resistance to TcdB-mediated cytotoxicity. Here, I will focus on the roles of two of the 43 genes identified in the screen, nectin-3 and galectin-3.

Thursday, Feb. 24
9:30 a.m.
Room A5305 MCN