VUMC faculty requested to take Transgenic Mouse/ES Cell Shared Resource Survey
Letter from From Mark Magnuson to VUSM faculty:
The discovery of CRISPR/Cas has led to a new, exciting and highly flexible means for performing targeted-mutagenesis directly in mouse embryos, reducing the need for many investigators to perform gene targeting in mESCs. However, homologous recombination using mESCs is still needed for more sophisticated strategies such as complex knock-in alleles. These evolving needs have prompted a discussion about the transgenic services that Vanderbilt investigators need, whether some of these services can be acquired commercially, what new services the TMESCSR may need to develop and how the development of these services should be funded.
The current services of the TMESCSR are:
1. Microinjections into single cell embryos
2. Preparation of mESCs for microinjection
3. mESC microinjections into blastocysts
4. Rederivation and assisted reproduction
5. Embryo and sperm cryopreservation
6. Long-term storage of frozen germplasm.
If you anticipate using mice in your research over the next 5 years, please take the time to visit the link below to complete a short survey.
Professor of Molecular Physiology and Biophysics,
Cell and Developmental Biology, and Medicine
Louise B. McGavock Chair
Director, Center for Stem Cell Biology
Sr. Scientific Co-Director, TMESCSR
You may open the survey in your web browser by clicking the link below: