Digestive Disease Research Center
Novel Cell Line Development Subcore
Director: Jeff Franklin, Ph.D.
Collaborator: Robert Whitehead, Ph.D.
Core Services:
- Utilizes the Immortomouse as a source of a conditional immortalizing gene in crosses with other transgenic mouse models that are relevant to a particular digestive disease
- Establishes conditionally-immortalized epithelial cell lines from gastrointestinal mucosa, pancreas orliver
- Fully characterizes these cell lines with respect to their epithelial phenotype, the presence of the specific genetic mutation of interest and the presence of tissue-specific markers
- Has established a “bank” of early passage cells for use by DDRC investigators
