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Eicosanoid Core Laboratory

 The Eicosanoid Core Laboratory is a Vanderbilt University Shared Resource designated for the analysis of oxidation products of arachidonic acid and other polyunsaturated fatty acids.  These molecules include isoprostanes, prostaglandins, leukotrienes, epoxyeicosatetraenoic acids (EETs), and hydroxy- and di-hydroxy-eicosatetraenoic acids (20-HETE and DHETs), and their metabolites.  The services available in the Core are listed in the Table below.  All of the assays performed are validated highly precise, accurate, and sensitive mass spectrometric assays, many of which were developed by Core personnel.

Analyte

Matrix

20-hydroxy-eicosatetraenoic acid (20-HETE)

Plasma, Tissues, Cells/Media

Di-hydroxy-eicosatetraenoic acids (DHETs)

Plasma, Tissues, Cells/Media

Epoxyeicosatetraenoic acids (EETs)

Plasma, Tissues, Cells/Media

F2-Isoprostanes

Plasma, Urine, Tissues, Cells/Media, CSF, BAL

F2-Isoprostane metabolite

 Urine

F3-Isoprostanes (from eicosapentaenoic acid)

Plasma, Urine, Tissues, Cells/Media, CSF, BAL

F4-Isoprostanes/Neuroprostanes (from eicosapentaenoic acid)

Plasma, Urine, Tissues, Cells/Media, CSF, BAL

Isofurans

Plasma, Urine, Tissues, Cells/Media, CSF, BAL

Thromboxane B2

Serum

PGE2 metabolite

Urine

PGD2 metabolite

Urine

PGI2 (prostacyclin) metabolite

Urine

Thromboxane B2 metabolite

Urine

Leukotriene B4

Cells/Media

Leukotriene E4

Urine

Parent Prostaglandins (F, D, E, J, A, I, and thromboxane B2)

Tissues, Cells/Media, CSF, EBC, BAL

 

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