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Flow Cytometry Shared Resource

How long will it take to sort all my cells on your sorter?

     The answer to this question will vary greatly.  You can make some rough estimates that should put you in the ballpark.  For the typical low pressure sort with the 100 um nozzle you can estimate that we'll run a threshold rate of about 3000 cells/second, this translates to about 10 million cells in one hour.  For the typical high pressure sort with the 70 um nozzle you can estimate around 20,000 cells/second, translating to around 70 million cells in one hour.  These estimates are conservative and speculative, we'll make adjustments to the rate of cells/second based on the efficiency and quality of the sort in relation to each investigators needs and the situation.

This page was last updated April 23, 2007 and is maintained by