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Which pressure/nozzle settings are right for my sort?

     We will choose the nozzle size for your particular sort.  This will depend on the size and characteristics of the cells more than anything else.  The nozzle size should be about four to five times that of the cells that are being sorted.  For sorts targeting most lymphocytes we'll probably run the 70 um nozzle.  For larger cells we'll probably choose the 100 um nozzle.  Increased pressure and the smaller orifice of the 70 um nozzle will expose your cells to a slightly harsher evironment, albeit very briefly.  If your cells are more delicate, we might choose the 100 um nozzle regardless of their size.
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This page was last updated April 23, 2007 and is maintained by Kevin Weller