Technology Research and Development projects in Imaging Mass Spectrometry are ongoing to expand the capabilities of the technology, enabling new application areas. Current projects include the development of new instrumental capabilities through modification of commercial instruments to increase imaging resolution, improving sample preparation techniques for different classes of analytes, and enabling reliable analysis of discrete cell populations.
Histology Directed Molecular Analysis
MALDI MS profiling and imaging has an advantage over other MS-based methods because molecules of interest in tissue remain in their natural environment, preserving their spatial relationship. This technology is being focused in the area of the anatomic pathology workflow to better understand disease mechanisms and resolve ambiguous diagnoses.
High Spatial Resolution Imaging
Commercial MALDI Mass Spectrometers are capable of routine imaging at resolutions greater than 25 micrometers. We are developing the means to reach spatial resolutions between 0.5 and 5.0 micrometers on a routine basis by modifying commercial instruments with custom optical and mechanical systems.
High Sensitivity Imaging
New capabilities of Imaging Mass Spectrometry are being developed to new classes of analytes and significant sensitivity enhancement through the use of novel and improved sample preparation and by the application of modern mass spectrometry techniques.
Surface Chemistry Enhanced IMS
Sample preparation in Imaging Mass Spectrometry is critically important to ensure reproducible tissue images with the highest achievable spatial resolution; however, current methods are often highly variable and time consuming. We are developing protocols for both matrix application and matrix-free sample preparation that provide high analyte specificity and high image fidelity across multiple preparations.