Evernote, how we love thee. Clip web notes, share notebooks and replace your hippocampus entirely with this great app. Oh, and did we mention its free?
Google Founder Sergey Brin's Quest to Increase Bioinformatic Processing of Neurodegenerative Disease Genes. Excellent Wired article here.
Protocols
General Guidelines
We have provided many of our most commonly used protocols as a resource to those who wish to confirm our results or expand on our published work. We make every effort to post the most up-to-date information possible. If you are working on a particular technique and have any questions or concerns, please contact us and we will make every effort to put you in touch with a team member who may be working with the method currently.
Know that an informed decision on your part to modify a protocol from what is our current lab practice means you will face certain tragic scientific defeat, or at least a lack of assistance from us and a snarky response to inquires, as we aren't in the business of reinventing wheels. We leave that to Firestone, and they seem to be having enough problems.
Now enjoy a protocol or two thousand on us.
Protocols
New member orientation
All new members of the team are expected to ensure they are compliant with VUMC policies for safety, animal handling, human specimen work and that they have a full understanding of of lab maintenance and business policies. For more information, be sure you check the McLaughlin Lab Guidelines.
Molarity Quiz (to be turned into BethAnn McLaughlin):
Western Blot Information
Cell Culture
Primary Cultures
Cell Lines
Stable Transfection - From Rick "we love him!" Morimoto's Lab many good protocols there.
Biochemical Assays
Molecular Biology
Protein Chemistry
Documentation and Imaging
The Really Important Things
Autophagy Protocols
Dan Klionsky, you crazy super genius mildly grumpy dude.....we worship you! And having a protocol site just for those of us who love 'self eating' on the Autophagy journal site is fabulous. Check out the protocols here.
The Dissection Video
Neuronal dissection videos are available from companies like Stem Cell Technology
From this demo, you can get a sense of the prep though.
Lab buddy Miguel Perez-Pinzon organized the 2nd World Conference on Preconditioning. Check Translational Stroke Research February 2013 for our work. Also, an update in March 2012 Stroke here. Great links and resources!!
We support the excellent position statement of the Society for Neuroscience on the ethical and humane use of animals in lifesaving medical research. Find out more here
News from VMC
- Vanderbilt and AstraZeneca agree to collaborate on neuroscience drug discovery
Chronicle of Higher Education - 1/17/2013 - VUMC and West Tennessee Healthcare announce affiliation agreement
WPLN Nashville Public Radio - 1/17/2013 - Lee Ann Ruffing, director of operations in Children’s Services at the Monroe Carell Jr. Children’s Hospital at Vanderbilt, named to Emerging Leaders
Nashville Post - 1/17/2013 - Clinical enterprise well prepared for future challenges: Balser
The Reporter - 6/29/2012