1. The minimum number of animals recommended per group/treatment/diet/genotype is eight.
2. Unless your research question is specifically gender related all animals should be the same sex due to known effects of gender on energy balance.
3. At the time of testing animals should be fully mature (8 weeks+) and age matched as much as possible (±2 weeks). It is recommended that all animals be sent in a single shipment.
4. Current Vanderbilt quarantine for animals shipped from external institutions is approximately 6 weeks.
5. When comparing between genotypes (particularly on mixed genetic backgrounds) wild-type littermates should be used as controls.
6. Studies can be performed with animals on special diets but these diets must be received a minimum of 2 weeks prior to the start of the study. If the animals need to be on the diet while in quarantine; fenbendazole must be added to the diet. If fenbendazole is contraindicated for your mice, contact the core director.
7. Measurement of body composition is included in all indirect calorimetry studies.
8. Before starting experiments we need to know the genotype, genetic modification and background strain of the animals. This is required so we can make sure that the experiments are run correctly. **Please contact the core director for more details**.
9. Data analysis will include indirect calorimetry normalized to both body weight AND lean body mass. Normalization to lean mass is currently considered the best way to present data. Please refer to Butler et al Diabetes 2010 59(2):323-9 for a detailed discussion of the issues.
10. On completion of the study ear punches will be returned to the investigator for validation of genotypes.