Mouse Metabolic Phenotyping Center
Services List -
Metabolic Pathophysiology Core
Metabolic Pathophysiology Core
- Catheterization of Jugular Vein and Carotid Artery
- Cannulation of the Cerebral Ventricle
- Glucose Tolerance Test
- Hyperinsulinemic Clamp
- Hyperglycemic Clamp
- Glucose Turnover
- Hepatic Gluconeogenesis / Glycogenolysis
- Glycogen Synthesis
- Amino Acid Kinetics
- Tissue Specific Glucose Uptake
- Tissue Specific Fatty Acid Uptake
- Indirect Calorimetry/Energy Expenditure
- Metabolic Response to Exercise
- Spontaneous Exercise Activity
- Food Consumption
- Body Composition
- Exploratory Locomotor Activity
- Assessment of Real Time Imaging of Cellular Metabolic Events
- In vivo Optical Imaging of Gene Expression
- Isolation of murine pancreatic islets from normal and genetically modified mice
- Measurement of insulin secretion by pancreatic islets
MetabolicPathCore: Hyperglycemic
Hyperglycemic Clamp
The responsiveness of the pancreas to glucose is assessed using the hyperglycemic clamp. Hyperglycemic clamps are performed on conscious mice with catheters chronically implanted in the jugular vein and carotid artery. A defined hyperglycemic stimulus is created using a primed variable glucose infusion to raise the glucose level to twice basal for 120 min. An established priming algorithm is used to elevate glucose quickly. Mice with extra copies of the glucokinase gene locus were demonstrated to have a blunted insulin response to hyperglycemia using this technique (Niswender et al. J. Biol. Chem. 1997).
