Mouse Metabolic Phenotyping Center
Metabolic Pathophysiology Core
- Catheterization of Jugular Vein and Carotid Artery
- Cannulation of the Cerebral Ventricle
- Glucose Tolerance Test
- Hyperinsulinemic Clamp
- Hyperglycemic Clamp
- Glucose Turnover
- Hepatic Gluconeogenesis / Glycogenolysis
- Glycogen Synthesis
- Amino Acid Kinetics
- Tissue Specific Glucose Uptake
- Tissue Specific Fatty Acid Uptake
- Indirect Calorimetry/Energy Expenditure
- Metabolic Response to Exercise
- Spontaneous Exercise Activity
- Food Consumption
- Body Composition
- Exploratory Locomotor Activity
- Assessment of Real Time Imaging of Cellular Metabolic Events
- In vivo Optical Imaging of Gene Expression
- Isolation of murine pancreatic islets from normal and genetically modified mice
- Measurement of insulin secretion by pancreatic islets
MetabolicPathCore: TissueSpecific Fatty Acid
Tissue Specific Fatty Acid Uptake
Tissue fatty acid uptake is assessed by measuring tissue-specific incorporation of circulating 125I-BMIPP (Rottman et al. Am. J. Physiol. 2002). The beta-methyl modification of the long-chain fatty acid BMIPP (beta-methyl-p-iodophenylpentadecanoic acid) causes terminal trapping in the TCA cycle. Studies in man and a variety of small animal models, including rodents, have shown that BMIPP uptake and metabolism closely tracks that of endogenous long-chain fatty acids in a variety of normal and pathophysiologic states.
BMIPP is dissolved in proprionic acid, and incorporation of 125I is catalyzed with CuSO4. After extraction, the purified 125I-BMIPP is dissolved in ursodeoxycholic acid, filtered, and adjusted to defined activity. This stable compound is suitable for direct intravascular injection. Serum levels are stable in tracer amounts after injection, and tissue incorporation is measured by gamma counting of freeze-clamped samples in protocols compatible with the simultaneous assay of, for example, [2-3H]DG.
