The analysis of complex proteomes of tissue and biofluid samples provides information that can facilitate the detection of disease states, responses to therapy and drug toxicity.
Proteome profiling identifies mass spectral patterns derived from multiple proteins in a tissue or biofluid sample. Proteome imaging can be done by analyzing mass spectral data acquired across a grid within a tissue section. Recent work suggests that proteome profiling can identify biomarkers of diseases, including different cancers.
The Tissue Core laboratory provides direct proteome profiling and protein imaging of intact tissues by MALDI-MS. The Core also provides proteome profiling of biofluids by MALDI-MS as they pertain to a specific disease.
Proteomic patterns can be analyzed with bioinformatics and biostatistical methods to identify sets of diagnostic spectral markers. Identification of the proteins and protein fragments that account for biomarker signals is done in collaboration with the Proteomics Core Laboratory.
This new Core Laboratory was established as a Shared Resource in the Vanderbilt Ingram Cancer Center. Technology for tissue proteome profiling and imaging by MALDI-MS was developed in the Research and Development Laboratory in the MSRC. Technology and methods for tissue and serum are rapidly evolving and new methods developed to improve the sensitivity and throughput of these approaches.