MSRC Schey Lab

Proteomics Methods Development & Applications

The focus of the Schey lab is on the use of mass spectrometry in proteomics and in protein structure determination. The lab is interested in both method/instrument development as well as in applications of state-of-the-art technology to biomedical science. Specific projects are focused on lens protein structure/function in aging and cataractogenesis, imaging mass spectrometry, biomarker discovery via exosome analysis, and shrimp & oyster innate immunity. Other areas of interest include ocular proteomics (retina and glaucoma), signal transduction and cardiovascular proteomics.

Some recent publications:

Wang Z, Hill S, Luther JM, Hachey DL, Schey KL. Proteomic Analysis of Urine Exosomes by Multidimensional Protein Identification Technology (MudPIT). Proteomics 12:329-338, 2012.

Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL. Protein Profile of Exosomes from Trabecular Meshwork Cells, J. Proteomics 74:796-804, 2011.

Gutierrez DB, Garland D, Schey KL. Spatial analysis of human lens aquaporin-0 post-translational modifications by MALDI mass spectrometry tissue profiling, Exp. Eye Res., 93:912-920, 2011.

Grey AC, Crouch RK, Koutalos Y, Schey KL, and Ablonczy Z. Spatial Localization of A2E in the Retinal Pigment Epithelium, Invest. Ophthalmol. Vis. Sci. 52:3926-3933, 2011.

Wang Z and Schey KL. Aquaporin-0 interacts with the FERM domain of ERM proteins in the ocular lens, Invest. Ophthalmol. Vis. Sci. 52:5079-5087, 2011.

Truscott RJW, Comte-Walters S, Ablonczy A, Schwacke JH, Berry Y, Korlimbinis A, Friedrich MG, Schey KL. Tight binding of proteins to membranes from older human cells. AGE, 33:543-54, 2011.

Reissner KJ, Uys JD, Schwacke JH, Comte-Walters S, Rutherford-Bethard JL, Dunn TE, Blumer JB, Schey KL, Kalivas PW. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. J. Neurosci., 31:5648-58, 2011.

Sakakura M, Hadziselimovic A, Wang Z, Schey KL, Sanders CR. Structural Basis for the Trembler-J Phenotype of Charcot-Marie-Tooth Disease, Structure 19:1160-1169, 2011.

Schey KL, Wang Z, Gutierrez DB, Wei J, Grey AC. Novel fatty acid acylation of lens integral membrane protein aquaporin-0. Biochemistry 49:9858-9865, 2010.

Qureshi A, Subathra M, Grey A, Schey K, Del Poeta M, Luberto C. Role of sphinghomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans. PLoS One, 5:e15587, 2010.