The focus of the Schey lab is on the use of mass spectrometry in proteomics and in protein structure determination. The lab is interested in both method/instrument development as well as in applications of state-of-the-art technology to biomedical science. Specific projects are focused on lens protein structure/function in aging and cataractogenesis, imaging mass spectrometry, biomarker discovery via exosome analysis, and shrimp & oyster innate immunity. Other areas of interest include ocular proteomics (retina and glaucoma), signal transduction and cardiovascular proteomics.
Wang Z, Hill S, Luther JM, Hachey DL, Schey KL. Proteomic Analysis of Urine Exosomes by Multidimensional Protein Identification Technology (MudPIT). Proteomics 12:329-338, 2012.
Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL. Protein Profile of Exosomes from Trabecular Meshwork Cells, J. Proteomics 74:796-804, 2011.
Gutierrez DB, Garland D, Schey KL. Spatial analysis of human lens aquaporin-0 post-translational modifications by MALDI mass spectrometry tissue profiling, Exp. Eye Res., 93:912-920, 2011.
Grey AC, Crouch RK, Koutalos Y, Schey KL, and Ablonczy Z. Spatial Localization of A2E in the Retinal Pigment Epithelium, Invest. Ophthalmol. Vis. Sci. 52:3926-3933, 2011.
Wang Z and Schey KL. Aquaporin-0 interacts with the FERM domain of ERM proteins in the ocular lens, Invest. Ophthalmol. Vis. Sci. 52:5079-5087, 2011.
Truscott RJW, Comte-Walters S, Ablonczy A, Schwacke JH, Berry Y, Korlimbinis A, Friedrich MG, Schey KL. Tight binding of proteins to membranes from older human cells. AGE, 33:543-54, 2011.
Reissner KJ, Uys JD, Schwacke JH, Comte-Walters S, Rutherford-Bethard JL, Dunn TE, Blumer JB, Schey KL, Kalivas PW. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. J. Neurosci., 31:5648-58, 2011.
Sakakura M, Hadziselimovic A, Wang Z, Schey KL, Sanders CR. Structural Basis for the Trembler-J Phenotype of Charcot-Marie-Tooth Disease, Structure 19:1160-1169, 2011.
Schey KL, Wang Z, Gutierrez DB, Wei J, Grey AC. Novel fatty acid acylation of lens integral membrane protein aquaporin-0. Biochemistry 49:9858-9865, 2010.
Qureshi A, Subathra M, Grey A, Schey K, Del Poeta M, Luberto C. Role of sphinghomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans. PLoS One, 5:e15587, 2010.