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MSRC Schey Lab

Kevin L. Schey, Ph.D.,
Professor of Biochemistry
Professor of Ophthalmology
Proteomics Core Director
Mass Spectrometry Core Director

kevin.schey@vanderbilt.edu

Tel: 615-936-6861
Fax: 615-343-8372

 

Proteomics Methods Development & Applications

The focus of the Schey lab is on the use of mass spectrometry in proteomics and in protein structure determination. The lab is interested in both method/instrument development as well as in applications of state-of-the-art technology to biomedical science. Specific projects are focused on lens protein structure/function in aging and cataractogenesis, imaging mass spectrometry, biomarker discovery via exosome analysis, and shrimp & oyster innate immunity. Other areas of interest include ocular proteomics (retina and glaucoma), signal transduction and cardiovascular proteomics.

Some recent publications:

Anderson DMG, Zblonczy A, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL. High Resolution 10µm MALDI- Imaging Mass Spectrometry of Retinal Tissue Lipids. J. Amer. Soc. Mass Spectrom., in press.

Wang Z, Lyons B, Truscott RJW, Schey KL. Human Protein Aging: Modification and Crosslinking through Dehydroalanine and Dehydrobutyrine Intermediates. Aging Cell, 13:226-234, 2014.

Schey KL, Wang Z, Wenke J, Qi Y. Aquaporins in the Eye: Expression, Function, and Roles in Ocular Disease. Biochim. Biophys. Acta, 1840:1513-1523, 2014.

Betke KM, Rose KL, Friedman DB, Baucom AJ, Hyde K, Schey KL, Hamm HE. Differential Localization of G Protein βγ Subunits. Biochemistry, 53:2329-2343, 2014.

Chaturvedi R, Asim M, Piazuilo MB, Yan F, Barry DP, Sierra JC, Delgado AG, Hill S, Casero RA, Bravo LE, Dominguez RL, Correa P, Polk DB, Washington MK, Rose KL, Schey KL, Peek RM, Morgan DR, Wilson KT. Activation of EGFR and ErbB2 by Helicobacter pylori Results in Survival of Gastric Epithelial Cells with DNA Damage. Gastroenterol. 146:1739-1751, 2014.

Burns TA, Dours-Zimmermann MT, Zimmermann DR, Krug EL, Comte-Walters S, Reyes L, Davis MA, Schey KL, Schwacke JH, Kern CB, Mjaatvedt CH. Imbalanced expression of Vcan mRNA splice form proteins laters heart morphology and cellular protein profiles. PLoS One 9(2):e89133. 2014.

Schey KL, Grey AC, Nicklay JJ. Mass Spectrometry Analysis of Membrane Proteins: A Focus on Aquaporins, Biochemistry, 52:3807-3817, 2013.

Roberson CD, Qi Y, Gercel-Taylor C, Schey KL, Taylor DD. Identification of specific tumor-reactive antigen using circulating free and tumor-derived exosome humoral responses. Exosomes and Microvesicles, 2013, 1:8. doi: 10.5772/57524.

Ablonczy Z, Higbee D, Anderson DM, Dahrouj M, Grey AC, Koutalos Y, Schey KL, Gutierrez D, Hanneken A, Crouch RK. Lack of Correlation between the Spatial Distribution of A2E and Lipofuscin Fluorescence in the Human Retinal Pigment Epithelium. Invest. Ophthalmol. Vis. Sci, 54:5535-542, 2013.

Nicklay JJ, Harris GA, Schey KL, Caprioli RM, MALDI imaging and in situ identification of integral membrane proteins from rat brain tissue sections, Anal Chem, 85:7191-7196, 2013.

Wang Z, Han J, David LL, Schey KL. Proteomics and Phosphoproteomics Analysis of Human Lens Fiber Cell Membranes, Invest. Ophthalmol. Vis. Sci. 54:1135-1143, 2013.

Anderson DMG, Mills D, Spraggins J, Lambert WS, Calkins DJ, Schey KL. High Resolution MALDI-Imaging Mass Spectrometry of Lipids in Rodent Optic Nerve Tissue, Mol. Vis. 19:581-592, 2013.

Schey KL, Anderson DMG, Rose KL. Spatially-Directed Protein Identification from Tissue Sections by Top-Down LC-MS/MS with Electron Transfer Dissociation. Anal. Chem., 85:6767-6774, 2013.

Grey, AC, Walker KL, Petrova RS, Han J, Wilmarth PA, David LL, Donaldson PJ, Schey KL. Verification and Spatial Localization of Aquaporin-5 in the Ocular Lens. Exp. Eye Res. 108:94-102, 2013.

Schey KL, Grey AC, Nicklay JJ. Mass Spectrometry Analysis of Membrane Proteins: A Focus on Aquaporins, Biochemistry, 52:3807-3817, 2013.

Maddala R, Nagendran T, de Ridder GG, Schey KL, Rao PV. L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins, PLoS One, 8:e64676, 2013.

Wang Z, Hill S, Luther JM, Hachey DL, Schey KL. Proteomic Analysis of Urine Exosomes by Multidimensional Protein Identification Technology (MudPIT). Proteomics 12:329-338, 2012.

Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL. Protein Profile of Exosomes from Trabecular Meshwork Cells, J. Proteomics 74:796-804, 2011.

Gutierrez DB, Garland D, Schey KL. Spatial analysis of human lens aquaporin-0 post-translational modifications by MALDI mass spectrometry tissue profiling, Exp. Eye Res., 93:912-920, 2011.

Grey AC, Crouch RK, Koutalos Y, Schey KL, and Ablonczy Z. Spatial Localization of A2E in the Retinal Pigment Epithelium, Invest. Ophthalmol. Vis. Sci. 52:3926-3933, 2011.

Wang Z and Schey KL. Aquaporin-0 interacts with the FERM domain of ERM proteins in the ocular lens, Invest. Ophthalmol. Vis. Sci. 52:5079-5087, 2011.

Truscott RJW, Comte-Walters S, Ablonczy A, Schwacke JH, Berry Y, Korlimbinis A, Friedrich MG, Schey KL. Tight binding of proteins to membranes from older human cells. AGE, 33:543-54, 2011.

Reissner KJ, Uys JD, Schwacke JH, Comte-Walters S, Rutherford-Bethard JL, Dunn TE, Blumer JB, Schey KL, Kalivas PW. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. J. Neurosci., 31:5648-58, 2011.

Sakakura M, Hadziselimovic A, Wang Z, Schey KL, Sanders CR. Structural Basis for the Trembler-J Phenotype of Charcot-Marie-Tooth Disease, Structure 19:1160-1169, 2011.

Schey KL, Wang Z, Gutierrez DB, Wei J, Grey AC. Novel fatty acid acylation of lens integral membrane protein aquaporin-0. Biochemistry 49:9858-9865, 2010.

Qureshi A, Subathra M, Grey A, Schey K, Del Poeta M, Luberto C. Role of sphinghomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans. PLoS One, 5:e15587, 2010.

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