The Schey lab has incorporated laser capture microdissection and imaging mass spectrometry into its arsenal of tools for spatially-resolved proteomics. We are developing methods for imaging lens crystallin proteins as well as for imaging integral membrane proteins. In a collaboration with Dr. Rosalie Crouch at the Medical University of South Carolina, we are imaging retinoid metabolites in retina tissue. Tissues that have been imaged in the Schey lab to date include: lens, retina, heart, brain, lung, kidney and whole shrimp.
Cover from Investigative Ophthalmology and Visual Science, September 2009, showing an array of human lens crystallin images acquired by MALDI imaging mass spectrometry.
Collage of tissue images acquired in the Schey lab from various projects.
Ablonczy Z, Gutierrez DB, Grey AC, Schey KL, Crouch RK. Molecule-specific imaging and quantitation of A2E in the RPE. Adv. Exp. Med. Biol. 723:75-71, 2012.
Gutierrez DB, Garland D, Schey KL. Spatial analysis of human lens aquaporin-0 post-translational modifications by MALDI mass spectrometry tissue profiling, Exp. Eye Res., 93:912-920, 2011.
Qureshi A, Grey A, Rose KL, Schey KL, Del Poeta M. Cryptococcus neoformans modulates extracellular killing by neutrophils. Frontiers in Microbiol. 2:193, 2011.
Grey AC, Crouch RK, Koutalos Y, Schey KL, and Ablonczy Z. Spatial Localization of A2E in the Retinal Pigment Epithelium, Invest. Ophthalmol. Vis. Sci. 52:3926-3933, 2011.
Qureshi A, Subathra M, Grey A, Schey K, Del Poeta M, Luberto C. Role of sphinghomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans. PLoS One, 5:e15587, 2010.
Stella DR, Floyd KA, Grey AC, Renfrow MB, Schey KL, Barnes S. Tissue localization and solubilities of αA-crystallin and its numerous C-terminal truncation products in pre- and post-cataractous ICR/f rat lenses. Invest. Ophthalmol. Vis. Sci., 51:5153-5161, 2010.
Uys JD, Grey AC, Wiggins A, Schwacke JH, Schey KL, Kalivas PW. Matrix-Assisted Laser Desorption/Ionization Tissue Profiling of Secretoneurin in the Nucleus Accumbens Shell from Cocaine Sensitized Rats. J. Mass Spectrom., 45:97-103, 2010.
Grey AC, Gelasco AK, Moreno-Rodriguez RA, Krug EL, Schey KL. Molecular Morphology of the Chick Heart Visualized by MALDI Imaging Mass Spectrometry. Anat. Rec., 293:821-8, 2010.
Grey AC and Schey KL. Age-related Changes in the Spatial Distribution of Human Lens α-Crystallin Products by MALDI Imaging Mass Spectrometry, Invest. Ophthalmol. Vis. Sci., 50:4319-4329, 2009.
Grey AC, Chaurand P, Caprioli, RM , Schey KL. MALDI Imaging Mass Spectrometry of Integral Membrane Proteins from Ocular Lens and Retinal Tissue, J. Proteom. Res., 8:3278-3283, 2009.
Grey AC and Schey KL. Distribution of Bovine and Rabbit Lens α-Crystallin Products by MALDI Imaging Mass Spectrometry, Mol. Vis., 2008, 14:171-9.
Thibault DB, Gillam CJ, Han J, Schey KL. MALDI Tissue Profiling of Integral Membrane Proteins from Ocular Tissues, J. Amer. Soc. Mass Spectrom., 19:814-22, 2008.
Wang Z, Han J, Schey KL. Spatial Differences in an Integral Membrane Proteome Detected in Laser Capture Microdissected Samples, J. Proteome Res., 7:2696-702, 2008.
Han J and Schey KL. MALDI Tissue Imaging of Ocular Lens α-Crystallin, Invest Ophthalmol Vis Sci, 2006, 47:2990-6.