Based on our discovery that shrimp histones possess antimicrobial activity, we have characterized several shrimp histones and their role in innate immunity. In addition, a collaboration with Dr. Jerome LaPeyre has led us to characterize a number of oyster proteins that play a role in host defense. We are interested in identifying other proteins that play a role in invertebrate immunity and to use quantitative proteomics methods to identify mechanisms of defense.
Itoh N, Xue QG, Schey KL, Li Y, Cooper R, LaPeyre J. Characterization of the major plasma protein of the eastern oyster, Crassostrea virginica, and a proposed role in host defense. Comp. Biochem. Physiol. B: Bicohem. Mol. Biol., 158:9-22., 2011.
Xue Q-G, Hellberg ME, Schey KL, Itoh N, Eytan RI, Cooper RI, LaPeyre J. A new lysozyme from the eastern oyster, Crassostrea virginica, and a possible evolutionary pathway for i-type lysozymes in bivalves from host defense to digestion. BMC Evolutionary Biology, 10:213, 2010.
Robalino J, Carnegie RB, O`Leary N, A. Ouvry-Patat SA, de la Vega E, Prior S, Gross PS, Browdy CL, Chapman RW, Schey KL, Warr G. Contributions of functional genomics and proteomics to the study of immune responses in the Pacific White Leg shrimp Litopenaeus vannamei, Vet. Immunol. Immunopathol., 128:110-118, 2009.
Xue Q, Itoh N, Schey KL, Cooper RK, LaPeyre JF. Evidence indicating the existence of a novel family of serine protease inhibitors potentially involved in marine invertebrate immunity, Fish and Shellfish Immunol., 27:250-259, 2009.
Ouvry Patat SA and Schey KL. Characterization of antimicrobial histone sequences and posttranslational modifications by mass spectrometry. J. Mass Spectrom., 2007, 42:664-674.
Xue Q, Waldrop GL, Schey KL, Itoh N, Ogawa M, Cooper RK, Losso JN, LaPeyre JF. A Novel Slow-Tight Binding Serine Protease Inhibitor from Eastern Oyster (Crassostrea virginica) Plasma Inhibits Protease of the Oyster Parasite Perkinsus marinus. Comp. Biochem. Physiol., 2006, 145: 16-26.
Xue Q, Itoh N, Schey KL, Li Y, Cooper RK, La Peyre JF, A New Lysozyme from the Eastern Oyster (Crassostrea virginica) Indicates Adaptive Evolution of i-Type Lysozymes in Bivalve Mollusks, Cell. Mol. Life Sci., 2007, 64:82-95.