.

Department of Pathology, Microbiology, and Immunology

 

 

  Paul E. Bock, Ph.D.

 

 

Professor

Dept. of Pathology, Microbiology and Immunology

 

 

  Contact Information

 

 

Office Location:

Stallworth Rehabilitation Hospital

Room 1205
  Phone (office):  615-343-9863

Phone (lab): 615-343-3085

 

Fax: 615-322-1855

E-mail: paul.bock@vanderbilt.edu

 

 

Campus Mail address: 

Pathology, Microbiology and Immunology -3rd Fl

MCN C-3321 (2561)

 
US Mailing address:
  Vanderbilt University School of Medicine

Dept. of Pathology, Microbiology and Immunology-3rd Fl

MCN C-3321

Nashville, TN 37232-2561

 

 

Kroh, HK, Panizzi, P, Tchaikovski, S, Baird, TR, Wei, N, Krishnaswamy, S, Tans, G, Rosing, J, Furie, B, Furie, BC, Bock, PE. Active site-labeled prothrombin inhibits prothrombinase in vitro and thrombosis in vivo. J Biol Chem, 286(26), 23345-56, 2011

Laha, M, Panizzi, P, Nahrendorf, M, Bock, PE. Engineering streptokinase for generation of active site-labeled plasminogen analogs. Anal Biochem, 415(2), 105-15, 2011

Wiles, KG, Panizzi, P, Kroh, HK, Bock, PE. Skizzle is a novel plasminogen- and plasmin-binding protein from Streptococcus agalactiae that targets proteins of human fibrinolysis to promote plasmin generation. J Biol Chem, 285(27), 21153-64, 2010

Kroh, HK, Panizzi, P, Bock, PE. Von Willebrand factor-binding protein is a hysteretic conformational activator of prothrombin. Proc Natl Acad Sci U S A, 106(19), 7786-91, 2009

Tharp, AC, Laha, M, Panizzi, P, Thompson, MW, Fuentes-Prior, P, Bock, PE. Plasminogen substrate recognition by the streptokinase-plasminogen catalytic complex is facilitated by Arg253, Lys256, and Lys257 in the streptokinase {beta}-domain and kringle 5 of the substrate. J Biol Chem, 284(29), 19511-21, 2009

Verhamme, IM, Bock, PE. Rapid-reaction kinetic characterization of the pathway of streptokinase.plasmin catalytic complex formation. J Biol Chem, 2008

Smith, SB, Verhamme, IM, Sun, MF, Bock, PE, Gailani, D. Characterization of Novel Forms of Coagulation Factor XIa: independence of factor XIa subunits in factor IX activation. J Biol Chem, 283(11), 6696-705, 2008

Hacisalihoglu, A, Panizzi, P, Bock, PE, Camire, RM, Krishnaswamy, S. Restricted Active Site Docking by Enzyme-bound Substrate Enforces the Ordered Cleavage of Prothrombin by Prothrombinase. J Biol Chem, 282(45), 32974-82, 2007

Bock, PE, Panizzi, P, Verhamme, IM. Exosites in the substrate specificity of blood coagulation reactions. J Thromb Haemost, 5 Suppl 1, 81-94, 2007

Kroh, HK, Tans, G, Nicolaes, GA, Rosing, J, Bock, PE. Expression of allosteric linkage between the sodium ion binding site and exosite I of thrombin during prothrombin activation. J Biol Chem, 282(22), 16095-104, 2007

Munnix, IC, Kuijpers, MJ, Auger, J, Thomassen, CM, Panizzi, P, van Zandvoort, MA, Rosing, J, Bock, PE, Watson, SP, Heemskerk, JW. Segregation of platelet aggregatory and procoagulant microdomains in thrombus formation: regulation by transient integrin activation. Arterioscler Thromb Vasc Biol, 27(11), 2484-90, 2007

Panizzi, P, Friedrich, R, Fuentes-Prior, P, Kroh, HK, Briggs, J, Tans, G, Bode, W, Bock, PE. Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. J Biol Chem, 281(2), 1169-78, 2006

Panizzi, P, Boxrud, PD, Verhamme, IM, Bock, PE. Binding of the COOH-terminal lysine residue of streptokinase to plasmin(ogen) kringles enhances formation of the streptokinase.plasmin(ogen) catalytic complexes. J Biol Chem, 281(37), 26774-8, 2006

Panizzi, P, Friedrich, R, Fuentes-Prior, P, Richter, K, Bock, PE, Bode, W. Fibrinogen substrate recognition by staphylocoagulase.(pro)thrombin complexes. J Biol Chem, 281(2), 1179-87, 2006

Bianchini, EP, Orcutt, SJ, Panizzi, P, Bock, PE, Krishnaswamy, S. Ratcheting of the substrate from the zymogen to proteinase conformations directs the sequential cleavage of prothrombin by prothrombinase. Proc Natl Acad Sci U S A, 102(29), 10099-104, 2005

Ogawa, T, Verhamme, IM, Sun, MF, Bock, PE, Gailani, D. Exosite-mediated substrate recognition of factor IX by factor XIa. The factor XIa heavy chain is required for initial recognition of factor IX. J Biol Chem, 280(25), 23523-30, 2005

Bean, RR, Verhamme, IM, Bock, PE. Role of the streptokinase alpha-domain in the interactions of streptokinase with plasminogen and plasmin. J Biol Chem, 280(9), 7504-10, 2005

Verhamme, IM, Bock, PE, Jackson, CM. The preferred pathway of glycosaminoglycan-accelerated inactivation of thrombin by heparin cofactor II. J Biol Chem, 279(11), 9785-95, 2004

Panizzi, P, Friedrich, R, Fuentes-Prior, P, Bode, W, Bock, PE. The staphylocoagulase family of zymogen activator and adhesion proteins. Cell Mol Life Sci, 61(22), 2793-8, 2004

Boxrud, PD, Bock, PE. Coupling of conformational and proteolytic activation in the kinetic mechanism of plasminogen activation by streptokinase. J Biol Chem, 279(35), 36642-9, 2004

Boxrud, PD, Verhamme, IM, Bock, PE. Resolution of conformational activation in the kinetic mechanism of plasminogen activation by streptokinase. J Biol Chem, 279(35), 36633-41, 2004

Anderson, PJ, Nesset, A, Bock, PE. Effects of activation peptide bond cleavage and fragment 2 interactions on the pathway of exosite I expression during activation of human prethrombin 1 to thrombin. J Biol Chem, 278(45), 44482-8, 2003

Anderson, PJ, Bock, PE. Role of prothrombin fragment 1 in the pathway of regulatory exosite I formation during conversion of human prothrombin to thrombin. J Biol Chem, 278(45), 44489-95, 2003

Bedsted, Tina, Swanson, Richard, Chuang, Yung-Jen, Bock, Paul E, Bj??rk, Ingemar, Olson, Steven T, . Heparin and calcium ions dramatically enhance antithrombin reactivity with factor IXa by generating new interaction exosites.. Biochemistry, 42, 8143-52, 2003

Flanagan, John J, Chen, Jui-Chang, Miao, Yiwei, Shao, Yuanlong, Lin, Jialing, Bock, Paul E, Johnson, Arthur E, . Signal recognition particle binds to ribosome-bound signal sequences with fluorescence-detected subnanomolar affinity that does not diminish as the nascent chain lengthens.. J Biol Chem, 278, 18628-37, 2003

Verhamme, Ingrid M, Olson, Steven T, Tollefsen, Douglas M, Bock, Paul E. Binding of exosite ligands to human thrombin. Re-evaluation of allosteric linkage between thrombin exosites I and II. J Biol Chem, 277(9), 6788-98, 2002

Pekovich, S R, Bock, P E, Hoover, R L. Thrombin-thrombomodulin activation of protein C facilitates the activation of progelatinase A. FEBS Lett, 494(1-2), 129-32, 2001

Monteiro, R. Q., Bock, P. E., Bianconi, M. L., and Zingali, R. B. Characterization of Bothrojaracin Interacion with Human Prothrombin. Protein Science, 10, 1897-1904, 2001

Boxrud, P D, Verhamme, I M, Fay, W P, Bock, P E. Streptokinase triggers conformational activation of plasminogen through specific interactions of the amino-terminal sequence and stabilizes the active zymogen conformation. J Biol Chem, 276(28), 26084-9, 2001

Johnson, A E, Chen, J C, Flanagan, J J, Miao, Y, Shao, Y, Lin, J, Bock, P E, . Structure, function, and regulation of free and membrane-bound ribosomes: the view from their substrates and products.. Cold Spring Harb Symp Quant Biol, 66, 531-41, 2001

Anderson, P J, Bock, P E. Biotin derivatives of D-Phe-Pro-Arg-CH2Cl for active-site-specific labeling of thrombin and other serine proteinases. Anal Biochem, 296(2), 254-61, 2001

Boxrud, P D, Fay, W P, Bock, P E. Streptokinase binds to human plasmin with high affinity, perturbs the plasmin active site, and induces expression of a substrate recognition exosite for plasminogen. J Biol Chem, 275(19), 14579-89, 2000

Anderson, PJ, Nesset, A, Dharmawardana, KR, Bock, PE. Role of proexosite I in factor Va-dependent substrate interactions of prothrombin activation. J Biol Chem, 275(22), 16435-42, 2000

Boxrud, P D, Bock, P E. Streptokinase binds preferentially to the extended conformation of plasminogen through lysine binding site and catalytic domain interactions. Biochemistry, 39(45), 13974-81, 2000

Anderson, PJ, Nesset, A, Dharmawardana, KR, Bock, PE. Characterization of proexosite I on prothrombin. J Biol Chem, 275(22), 16428-34, 2000

Dharmawardana, K R, Olson, S T, Bock, P E. Role of regulatory exosite I in binding of thrombin to human factor V, factor Va, factor Va subunits, and activation fragments. J Biol Chem, 274(26), 18635-43, 1999

Dharmawardana, K R, Bock, P E. Demonstration of exosite I-dependent interactions of thrombin with human factor V and factor Va involving the factor Va heavy chain: analysis by affinity chromatography employing a novel method for active-site-selective immobilization of serine proteinases. Biochemistry, 37(38), 13143-52, 1998

Bock, P E, Olson, S T, Bj??rk, I. Inactivation of thrombin by antithrombin is accompanied by inactivation of regulatory exosite I. J Biol Chem, 272(32), 19837-45, 1997

Hogg, PJ, Jackson, CM, Labanowski, JK, Bock, PE. Binding of fibrin monomer and heparin to thrombin in a ternary complex alters the environment of the thrombin catalytic site, reduces affinity for hirudin, and inhibits cleavage of fibrinogen. J Biol Chem, 271(42), 26088-95, 1996

Bock, P E, Day, D E, Verhamme, I M, Bernardo, M M, Olson, S T, Shore, J D. Analogs of human plasminogen that are labeled with fluorescence probes at the catalytic site of the zymogen. Preparation, characterization, and interaction with streptokinase. J Biol Chem, 271(2), 1072-80, 1996

Olson, ST, Bock, PE, Kvassman, J, Shore, JD, Lawrence, DA, Ginsburg, D, Bj??rk, I. Role of the catalytic serine in the interactions of serine proteinases with protein inhibitors of the serpin family. Contribution of a covalent interaction to the binding energy of serpin-proteinase complexes. J Biol Chem, 270(50), 30007-17, 1995

Olson, ST, Stephens, AW, Hirs, CH, Bock, PE, Bj??rk, I. Kinetic characterization of the proteinase binding defect in a reactive site variant of the serpin, antithrombin. Role of the P1'' residue in transition-state stabilization of antithrombin-proteinase complex formation. J Biol Chem, 270(17), 9717-24, 1995

Bock, P E. Thioester peptide chloromethyl ketones: reagents for active site-selective labeling of serine proteinases with spectroscopic probes. Methods Enzymol, 222, 478-503, 1993

Bj??rk, I, Ylinenj??rvi, K, Olson, ST, Bock, PE. Conversion of antithrombin from an inhibitor of thrombin to a substrate with reduced heparin affinity and enhanced conformational stability by binding of a tetradecapeptide corresponding to the P1 to P14 region of the putative reactive bond loop of the inhibitor. J Biol Chem, 267(3), 1976-82, 1992

Olson, ST, Bock, PE, Sheffer, R. Quantitative evaluation of solution equilibrium binding interactions by affinity partitioning: application to specific and nonspecific protein-heparin interactions. Arch Biochem Biophys, 286(2), 533-45, 1991

Carlisle, TL, Bock, PE, Jackson, CM. Kinetic intermediates in prothrombin activation. Bovine prethrombin 1 conversion to thrombin by factor X. J Biol Chem, 265(35), 22044-55, 1990

Bock, PE, Craig, PA, Olson, ST, Singh, P. Isolation of human blood coagulation alpha-factor Xa by soybean trypsin inhibitor-sepharose chromatography and its active-site titration with fluorescein mono-p-guanidinobenzoate. Arch Biochem Biophys, 273(2), 375-88, 1989

Shore, JD, Day, DE, Bock, PE, Olson, ST. Acceleration of surface-dependent autocatalytic activation of blood coagulation factor XII by divalent metal ions. Biochemistry, 26(8), 2250-8, 1987