.

Department of Pathology, Microbiology, and Immunology

 

 

   Pampee P. Young, M.D., Ph.D.

 

 

Associate Professor

Dept of Pathology, Microbiology and Immunology 

Dept of Medicine

Medical Director, Transfusion Service

Vanderbilt Medical Center and Department of Veterans Affairs

 

  

  Contact Information
 


  Office Location:

C-2217 MCN

Nashville, TN 37232-2561

Phone: 615-936-1098     

Fax: 615-343-7023

E-mail: pampee.young@vanderbilt.edu

 

Campus Mail address:

Pathology, Microbiology and Immunology-3rd Fl

C-3321 MCN (2561)

 

US Mailing address:

Vanderbilt University School of Medicine

Pathology, Microbiology and Immunology-3rd Fl

C-3321 MCN

Nashville, TN 37232-2561

 

 

 

Education

 

 

M.D., Univ of Texas Southwestern
Ph.D., Univ of Texas Southwestern (MSTP)
B.A., Rice Univ

 

 

Research Keywords

 

 

Endothelial Progenitor Cells (EPCs), Angiogenesis, Mesenchymal stem cells (MSCs), Vasculogenesis, cellular adaptation to biomechanical stress, tumor-host interactions, regeneneration, cardiac regeneration

 

 

Research Description

 

 



My laboratory studies the cellular drivers and signaling mechanisms that regulate tissue repair and regeneration. We use mouse models of myocardial and complex cutaneous tissue injuries to study endogenous regulators of repair as well as exogenous factors, including the role of stem cell-mediated cellular therapies, that regulate repair. My research interests dovetails well with my clinical role as Medical Director of Transfusion Medicine and of the Stem Cell Laboratory. In addition, I am dedicated to training future biomedical scientists and medical residents. Our long term goal is to identify signaling pathways which can be targeted to improve tissue repair.

Project 1

Role of Wnt signaling in repair vs. regeneration. Using both genetic models and novel small molecule Wnt pathway modulators, we have identified a pro-regenerative role in both cardiac and complex cutaneous wounds for the canonical Wnt pathway. Our current efforts are to dissect the cellular drivers of regeneration and to identify Wnt targets which mediate the pro-reparative phenotype.

Project 2

How do stem cells mediate repair and can we develop new bioengineered platforms to improve stem cell-based therapeutics? We have partnered with Vanderbilt Bioengeers to develop stem cell-based treatments for ischemic vascular diseases which seek to understand and optimize both stem cell and scaffold properties which result in improved blood flow restoration.

Project 3

We have developed a mouse model which demonstrates that deletion of a vascular smooth muscle cell protein, SPRR3, results in progression of atherosclerosis and ultimately to its most serious consequence, plaque rupture. Current projects are focused on identifying the molecular partners of SPRR3 and testing it as a novel therapeutic using a novel transgenic mouse as well as state-of-art peptide replacement approaches.

Project 4

As a Transfusion Medicine physician, I am involved in multiple clinical/translational projects involving blood and blood products in clinical medicine.

 

 

Clinical Research Description

 

 

The Vanderbilt University Blood Bank is undergoing automation as well as a major software installment. We continually strive to implement the best Transfusion Practices.

 

 

Publications

 

 

Young, PP, Sch??fer, R. Cell-based therapies for cardiac disease: a cellular therapist''s perspective. Transfusion, 2014

West, JD, Austin, ED, Gaskill, C, Marriott, S, Baskir, R, Bilousova, G, Jean, JC, Hemnes, AR, Menon, S, Bloodworth, NC, Fessel, JP, Kropski, JA, Irwin, D, Ware, LB, Wheeler, L, Hong, CC, Meyrick, B, Loyd, JE, Bowman, AB, Ess, KC, Klemm, DJ, Young, PP, Merryman, WD, Kotton, D, Majka, SM. Identification of a common Wnt-associated genetic signature across multiple cell types in pulmonary arterial hypertension. Am J Physiol Cell Physiol, 307(5), C415-30, 2014

Oakley, FD, Woods, M, Arnold, S, Young, PP. Transfusion reactions in pediatric compared with adult patients: a look at rate, reaction type, and associated products. Transfusion, 2014

Segedy, AK, Pyle, AL, Li, B, Zhang, Y, Babaev, VR, Jat, P, Fazio, S, Atkinson, JB, Linton, MF, Young, PP. Identification of small proline-rich repeat protein 3 as a novel atheroprotective factor that promotes adaptive Akt signaling in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol, 34(12), 2527-36, 2014

Gehrie, E, Muranyi, A, Walk, E, Young, PP. Commercially available blood-group antibodies may be used to visualize blood group A antigen in paraffin-embedded tissue sections. Arch Pathol Lab Med, 138(2), 155, 2014

Saraswati, S, Guo, Y, Atkinson, J, Young, PP. Prolonged hypoxia induces MCT4 expression in MSCs resulting in a secretome that is deleterious to cardiovascular repair. Stem Cells, 2014

Cohen, DN, Johnson, MS, Liang, WH, McDaniel, HL, Young, PP. Clinically significant hemolytic disease of the newborn secondary to passive transfer of anti-D from maternal RhIG. Transfusion, 54(11), 2863-6, 2014

Ardestani, S, Deskins, DL, Young, PP. Membrane TNF-alpha-activated programmed necrosis is mediated by Ceramide-induced reactive oxygen species. J Mol Signal, 8(1), 12, 2013

Gehrie, EA, Nian, H, Young, PP. Brown Recluse spider bite mediated hemolysis: clinical features, a possible role for complement inhibitor therapy, and reduced RBC surface glycophorin A as a potential biomarker of venom exposure. PLoS One, 8(9), e76558, 2013

Ardestani, S, Li, B, Deskins, DL, Wu, H, Massion, PP, Young, PP. Membrane versus soluble isoforms of TNF-?? exert opposing effects on tumor growth and survival of tumor-associated myeloid cells. Cancer Res, 73(13), 3938-50, 2013

Deskins, DL, Bastakoty, D, Saraswati, S, Shinar, A, Holt, GE, Young, PP. Human mesenchymal stromal cells: identifying assays to predict potency for therapeutic selection. Stem Cells Transl Med, 2(2), 151-8, 2013

Janz, DR, Zhao, Z, Koyama, T, May, AK, Bernard, GR, Bastarache, JA, Young, PP, Ware, LB. Longer storage duration of red blood cells is associated with an increased risk of acute lung injury in patients with sepsis. Ann Intensive Care, 3(1), 33, 2013

Alfaro, MP, Deskins, DL, Wallus, M, DasGupta, J, Davidson, JM, Nanney, LB, A Guney, M, Gannon, M, Young, PP. A physiological role for connective tissue growth factor in early wound healing. Lab Invest, 93(1), 81-95, 2013

McClain, CM, Hughes, J, Andrews, JC, Blackburn, J, Sephel, S, France, D, Viele, M, Goodnough, LT, Young, PP. Blood ordering from the operating room: turnaround time as a quality indicator. Transfusion, 53(1), 41-8, 2013

Hughey, CC, Alfaro, MP, Belke, DD, Rottman, JN, Young, PP, Wasserman, DH, Shearer, J. Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells. Cell Regen (Lond), 1(1), 3, 2012

Deskins, DL, Ardestani, S, Young, PP. The polyvinyl alcohol sponge model implantation. J Vis Exp(62), 2012

Gehrie, EA, Cates, JM, Nian, H, Olson, SJ, Young, PP. Blood Group A antigen expression on cardiac endothelium is highly individualized: possible implications for transplantation. Cardiovasc Pathol, 22(4), 251-6, 2012

Alfaro, MP, Young, PP. Lessons from genetically altered mesenchymal stem cells (MSCs): candidates for improved MSC-directed myocardial repair. Cell Transplant, 21(6), 1065-74, 2012

O''Leary, MF, Szklarski, P, Klein, TM, Young, PP. Hemolysis of red blood cells after cell washing with different automated technologies: clinical implications in a neonatal cardiac surgery population. Transfusion, 51(5), 955-60, 2011

Alfaro, MP, Saraswati, S, Young, PP. Molecular mediators of mesenchymal stem cell biology. Vitam Horm, 87, 39-59, 2011

Li, B, Pozzi, A, Young, PP. TNFalpha accelerates monocyte to endothelial transdifferentiation in tumors by the induction of integrin alpha5 expression and adhesion to fibronectin. Mol Cancer Res, 9(6), 702-11, 2011

Young, PP, Cotton, BA, Goodnough, LT. Massive transfusion protocols for patients with substantial hemorrhage. Transfus Med Rev, 25(4), 293-303, 2011

Garcia, CB, Shaffer, CM, Alfaro, MP, Smith, AL, Sun, J, Zhao, Z, Young, PP, Vansaun, MN, Eid, JE. Reprogramming of mesenchymal stem cells by the synovial sarcoma-associated oncogene SYT-SSX2. Oncogene, 2011

Gehrie, E, Neff, AT, Ciombor, KK, Harris, N, Seegmiller, AC, Young, PP. Profound piperacillin-mediated drug-induced immune hemolysis in a patient with cystic fibrosis. Transfusion, 2011

Saraswati, S, Deskins, DL, Holt, GE, Young, PP. Pyrvinium, a potent small molecule Wnt inhibitor, increases engraftment and inhibits lineage commitment of mesenchymal stem cells (MSCs). Wound Repair Regen, 20(2), 185-93, 2011

Cotton, BA, Dossett, LA, Haut, ER, Shafi, S, Nunez, TC, Au, BK, Zaydfudim, V, Johnston, M, Arbogast, P, Young, PP. Multicenter validation of a simplified score to predict massive transfusion in trauma. J Trauma, 69 Suppl 1, S33-9, 2010

Cates, JM, Friedman, DB, Seeley, EH, Dupont, WD, Schwartz, HS, Holt, GE, Caprioli, RM, Young, PP. Proteomic analysis of osteogenic sarcoma: association of tumour necrosis factor with poor prognosis. Int J Exp Pathol, 2010

Alfaro, MP, Vincent, A, Saraswati, S, Thorne, CA, Hong, CC, Lee, E, Young, PP. sFRP2 suppression of bone morphogenic protein (BMP) and Wnt signaling mediates mesenchymal stem cell (MSC) self-renewal promoting engraftment and myocardial repair. J Biol Chem, 285(46), 35645-53, 2010

Saraswati, S, Alfaro, MP, Thorne, CA, Atkinson, J, Lee, E, Young, PP. Pyrvinium, a potent small molecule Wnt inhibitor, promotes wound repair and post-MI cardiac remodeling. PLoS One, 5(11), e15521, 2010

Pyle, AL, Young, PP. Atheromas feel the pressure: biomechanical stress and atherosclerosis. Am J Pathol, 177(1), 4-9, 2010

Pyle AL, Li B, Maupin AB, Guzman RJ, Crimmins DL, Olson S, Atkinson JB and Young PP. . Biomechanical stress induces novel arterial intima-enriched genes: implications for vascular adaptation to stress. . Cardiovasc Pathol, in press, 2009

Cotton, BA, Au, BK, Nunez, TC, Gunter, OL, Robertson, AM, Young, PP. Predefined massive transfusion protocols are associated with a reduction in organ failure and postinjury complications. J Trauma, 66(1), 41-8; discussion 48-9, 2009

Li, B, Vincent, A, Cates, J, Brantley-Sieders, DM, Polk, DB, Young, PP. Low levels of tumor necrosis factor alpha increase tumor growth by inducing an endothelial phenotype of monocytes recruited to the tumor site. Cancer Res, 69(1), 338-48, 2009

Au, BK, Dutton, WD, Zaydfudim, V, Nunez, TC, Young, PP, Cotton, BA. Hyperkalemia following massive transfusion in trauma. J Surg Res, 157(2), 284-9, 2009

Li B, Vincent A, Cates J, Brantley-Sieders, D, Polk DB, Young PP. Low levels of TNF increase tumor growth by inducing an endothelial phenotype of monocytes recruited to the tumor site. Cancer Res, in press, 2009

Barnes, SL, Young, PP, Miga, MI. A novel model-gel-tissue assay analysis for comparing tumor elastic properties to collagen content. Biomech Model Mechanobiol, 8(4), 337-43, 2009

Alfaro, MP, Pagni, M, Vincent, A, Atkinson, J, Hill, MF, Cates, J, Davidson, JM, Rottman, J, Lee, E, Young, PP. The Wnt modulator sFRP2 enhances mesenchymal stem cell engraftment, granulation tissue formation and myocardial repair. Proc Natl Acad Sci U S A, 105(47), 18366-71, 2008

Pyle, AL, Li, B, Maupin, AB, Guzman, RJ, Crimmins, DL, Olson, S, Atkinson, JB, Young, PP. Biomechanical stress induces novel arterial intima-enriched genes: implications for vascular adaptation to stress. Cardiovasc Pathol, 19(2), e13-20, 2008

Alfaro MP, Pagni M, Vincent A, Atkinson J, Hill MF, Cates J, Davidson JM, Rottman J, Lee E, and Young PP. . A Wnt modulator sFRP2 enhances mesenchymal stem cell engraftment, granulation tissue formation and myocardial repair. . Proc. Natl. Acad. Sci. , USA, Track 2, in press, 2008

Gunter, OL, Au, BK, Isbell, JM, Mowery, NT, Young, PP, Cotton, BA. Optimizing outcomes in damage control resuscitation: identifying blood product ratios associated with improved survival. J Trauma, 65(3), 527-34, 2008

Pyle, AL, Atkinson, JB, Pozzi, A, Reese, J, Eckes, B, Davidson, JM, Crimmins, DL, Young, PP. Regulation of the atheroma-enriched protein, SPRR3, in vascular smooth muscle cells through cyclic strain is dependent on integrin alpha1beta1/collagen interaction. Am J Pathol, 173(5), 1577-88, 2008

Devin JK, Vaughan DE, Blevins LS, Chen Q, Covington J, Verity DK, and Young PP. Low-dose growth hormone administration mobilizes endothelial progenitor cells in healthy adults. Growth Hormone and IGF Res., 9, 983-92, 2008

Cotton, BA, Gunter, OL, Isbell, J, Au, BK, Robertson, AM, Morris, JA, St Jacques, P, Young, PP. Damage control hematology: the impact of a trauma exsanguination protocol on survival and blood product utilization. J Trauma, 64(5), 1177-82; discussion 1182-3, 2008

Young, PP, Vaughan, DE, Hatzopoulos, AK. Biologic properties of endothelial progenitor cells and their potential for cell therapy. Prog Cardiovasc Dis, 49(6), 421-9, 2007

Santoro, SA, Mosse, CA, Young, PP. The MD/PhD pathway to a career in laboratory medicine. Clin Lab Med, 27(2), 425-34; abstract ix, 2007

Ryzhov, S, Solenkova, NV, Goldstein, AE, Lamparter, M, Fleenor, T, Young, PP, Greelish, JP, Byrne, JG, Vaughan, DE, Biaggioni, I, Hatzopoulos, AK, Feoktistov, I. Adenosine Receptor Mediated Adhesion of Endothelial Progenitors to Cardiac Microvascular Endothelial Cells. Circ Res, 2007

Sharpe, EE, Teleron, AA, Li, B, Price, J, Sands, MS, Alford, K, Young, PP. The origin and in vivo significance of murine and human culture-expanded endothelial progenitor cells. Am J Pathol, 168(5), 1710-21, 2006

Li, B, Sharpe, EE, Maupin, AB, Teleron, AA, Pyle, AL, Carmeliet, P, Young, PP. VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization. FASEB J, 20(9), 1495-7, 2006

Young, PP, Modur, V, Teleron, AA, Ladenson, JH. Enrichment of genes in the aortic intima that are associated with stratified epithelium: implications of underlying biomechanical and barrier properties of the arterial intima. Circulation, 111(18), 2382-90, 2005

Teleron, Amylynn A, Carlson, Brian, Young, Pampee P. Blood donor white blood cell reduction filters as a source of human peripheral blood-derived endothelial progenitor cells. Transfusion, 45(1), 21-5, 2005

Young, Pampee P, Fantz, Corinne R, Sands, Mark S. VEGF disrupts the neonatal blood-brain barrier and increases life span after non-ablative BMT in a murine model of congenital neurodegeneration caused by a lysosomal enzyme deficiency. Exp Neurol, 188(1), 104-14, 2004

Alcorn, JL, Islam, KN, Young, PP, Mendelson, CR. Glucocorticoid inhibition of SP-A gene expression in lung type II cells is mediated via the TTF-1-binding element. Am J Physiol Lung Cell Mol Physiol, 286(4), L767-76, 2004

Young, PP, Uzieblo, A, Trulock, E, Lublin, DM, Goodnough, LT. Autoantibody formation after alloimmunization: are blood transfusions a risk factor for autoimmune hemolytic anemia. Transfusion, 44(1), 67-72, 2004

Young, Pampee P, Vogler, Carole, Alex Hofling, A, Sands, Mark S, . Biodistribution and efficacy of donor t lymphocytes in a murine model of lysosomal storage disease.. Mol Ther, 7, 52-61, 2003

Young, Pampee P, Hofling, A Alex, Sands, Mark S, . VEGF increases engraftment of bone marrow-derived endothelial progenitor cells (EPCs) into vasculature of newborn murine recipients.. Proc Natl Acad Sci U S A, 99, 11951-6, 2002

Young, P P, Goodnough, L T, Westervelt, P, Diersio, J F, . Immune hemolysis involving non-ABO/RhD alloantibodies following hematopoietic stem cell transplantation.. Bone Marrow Transplant, 27, 1305-10, 2001

Young, P P, Buller, R S, Storch, G A, . Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.. J Clin Microbiol, 38, 4260-1, 2000

Mendelson, C R, Gao, E, Li, J, Young, P P, Michael, L F, Alcorn, J L, . Regulation of expression of surfactant protein-A.. Biochim Biophys Acta, 1408, 132-49, 1998

Mendelson, C R, Gao, E, Young, P P, Michael, L F, Alcorn, J L, . Transcriptional regulation of the surfactant protein-A gene in fetal lung.. Chest, 111, 96S-104S, 1997

Young, P P, Mendelson, C R, . A GT box element is essential for basal and cyclic adenosine 3',5'-monophosphate regulation of the human surfactant protein A2 gene in alveolar type II cells: evidence for the binding of lung nuclear factors distinct from Sp1.. Mol Endocrinol, 11, 1082-93, 1997

Young, PP, Mendelson, CR. A CRE-like element plays an essential role in cAMP regulation of human SP-A2 gene in alveolar type II cells. Am J Physiol, 271(2 Pt 1), L287-99, 1996