.

Department of Pathology, Microbiology, and Immunology

 

 

Mark R. Boothby, M.D., Ph.D.

 

 

Professor

Dept. of Pathology, Microbiology and Immunology

Professor

Dept. of Medicine

 

 

Contact Information

 

 

Office Location:
MCN AA-4214B

Phone: 615-343-1699
E-mail:  mark.boothby@vanderbilt.edu

 

Campus Mail address:

Pathology, Microbiology and Immunology-5th Fl

MCN A-5301 (2363)

 

US Mailing address:
Vanderbilt University School of Medicine
Dept of Pathology, Microbiology and Immunology-5th Fl
MCN A-5301
Nashville, TN 37232-2363

 

 

 

Education

 

 

M.D., Ph.D., Washington Univ. St. Louis
B.S. , University of Wisconsin - Madison

 

 

Research Keywords

 

 

signal transduction; cytokine receptor; cellular metabolism; gene regulation, transcription factors; gene transcription; chromatin; epigenetics; epigenetic regulation; Chromatin modifications; differentiation; transgenic mice; knock-out; retroviral transduction; T lymphocyte; immune disease models, allergic diseases, Immunology, Pulmonary

 

 

Research Description

 

 

We investigate mechanisms by which signaling and transcription factors regulate cell growth, differentiation, and survival. The lab dissects these questions through an integration of genetic manipulations, biochemical studies, and analyses of function in immunity.

Potential projects (2013):
1. Investigation of a novel protein cloned in the lab, which enhances the function of a transcription factor central to allergic diseases and asthma. We are using knockout mice deficient in this cofactor, a novel ADP-ribosyltransferase, to explore how this protein impacts signaling to the regulation of programmed cell death, cellular metabolism, B lymphoma pathophysiology (because of connections to a related protein affecting the outcome of lymphocyte cancers), and the impact of this protein on vaccination and immunity to pathogens.
2. Signaling and immune function mediated by the Akt / mTOR axis of serine-threonine kinases. We have identified evidence of an important role for a novel complex involving the kinase mTOR - "mammalian target of rapamycin" (rapamycin being an important immune suppressant drug) - in T cell differentiation and are dissecting molecular mechanisms involved in these findings as well as impact on leukemia development and on immunity, including T cell memory, B cell development and function in models of vaccination and anti-pathogen immunity, and in autoimmunity.
3. Regulation of cytokine gene transcription. Studies of particular excitement at present include working to understand the molecular and epigenetic basis for two processes we have identified for immunological memory as carried by cells in the T helper 2 subset.

One major focus is on the differentiation of naive T helper lymphocytes into activated cells that have acquired effector functions, i.e., the ability to express new genes that orchestrate immune responses. One subset of effector T helper cells is called the Th2 subset. Differentiation into Th2 effectors is driven by the cytokine IL-4, so one major area of study in the lab is the transduction of signals by the IL-4 receptor and the means by which IL-4 regulates gene transcription. We cloned and knocked out a gene encoding a novel protein that enhances IL-4 induction of transcription and pro-survival signaling. Future studies will include determining the biochemical and function roles of this novel transcriptional coregulator.

We recently discovered that memory Th2 cells permit an unexpected flexibility in regulation of the Th1 cytokine gene for IFN-gamma while at the same time retaining commitment to expression of the characteristic Th2 cytokine genes (IL-4, IL-5, and IL-13). We will dissect molecular mechanisms for both flexibility and commitment, using analyses of transcription factor knockouts as well as of chromatin (epigenetic DNA methylation as well as histone modifications).

A second class of effector T cells is known as the Th1 subset, of which a hallmark is the activation of the gene encoding the inflammatory cytokine interferon-gamma. We dicsoveredthat the transcription factor NF-kappaB is particularly important in the Th1 response but not in responses mediated by Th2 cells, and have identified an orderly sequence of molecular events leading to remodeling of the IFN-gamma promoter. Using analyses of chromatin modifications and structure in the interferon gamma locus, we are in the process of determining the mechanisms by which the gene is regulated in primary T helper subsets, memory CD4 cells, and exploring the impact of these processes on differentiation of another autoimmunity-promoting class of T cells (Th17 cells).

Finally, we are in the midst of developing exciting data for both T and B lymphocytes in relation to the signaling pathways described above and dissecting how these pathways impact the generation and persistence of antibody-producing cells and immune memory.

 

 

Publications

 

 

Collier, SP, Collins, PL, Williams, CL, Boothby, MR, Aune, TM. Cutting edge: influence of Tmevpg1, a long intergenic noncoding RNA, on the expression of Ifng by Th1 cells. J Immunol, 189(5), 2084-8, 2012

Yang, J, Kantrow, S, Sai, J, Hawkins, OE, Boothby, M, Ayers, GD, Young, ED, Demicco, EG, Lazar, AJ, Lev, D, Richmond, A. Ikk4a/Arf inactivation with activation of the NF-??B/IL-6 pathway is sufficient to drive the development and growth of angiosarcoma. Cancer Res, 72(18), 4682-95, 2012

Volanakis, EJ, Boothby, MR, Sherr, CJ. Epigenetic regulation of the Ink4a-Arf (Cdkn2a) tumor suppressor locus in the initiation and progression of Notch1 driven T-ALL. Exp Hematol, 2012

Boothby, M, Williams, CL. The goldilocks effect. Sci Transl Med, 4(163), 163fs42, 2012

Lee, K, Nam, KT, Cho, SH, Gudapati, P, Hwang, Y, Park, DS, Potter, R, Chen, J, Volanakis, E, Boothby, M. Vital roles of mTOR complex 2 in Notch-driven thymocyte differentiation and leukemia. J Exp Med, 209(4), 713-28, 2012

Zhuang, G, Song, W, Amato, K, Hwang, Y, Lee, K, Boothby, M, Ye, F, Guo, Y, Shyr, Y, Lin, L, Carbone, DP, Brantley-Sieders, DM, Chen, J. Effects of cancer-associated EPHA3 mutations on lung cancer. J Natl Cancer Inst, 104(15), 1182-97, 2012

Cho, SH, Ahn, AK, Bhargava, P, Lee, CH, Eischen, CM, McGuinness, O, Boothby, M. Glycolytic rate and lymphomagenesis depend on PARP14, an ADP ribosyltransferase of the B aggressive lymphoma (BAL) family. Proc Natl Acad Sci U S A, 108(38), 15972-7, 2011

Gordy, LE, Bezbradica, JS, Flyak, AI, Spencer, CT, Dunkle, A, Sun, J, Stanic, AK, Boothby, MR, He, YW, Zhao, Z, Van Kaer, L, Joyce, S. IL-15 Regulates Homeostasis and Terminal Maturation of NKT Cells. J Immunol, 187(12), 6335-45, 2011

Dunaway, CM, Hwang, Y, Lindsley, CW, Cook, RS, Wu, JY, Boothby, M, Chen, J, Brantley-Sieders, DM. Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol Cell Biol, 31(3), 404-16, 2011

Boothby, M. CRACking the code without Rosetta: molecular regulation of calcium-stimulated gene transcription after T cell activation. J Immunol, 185(9), 4969-71, 2010

Lee, K, Gudapati, P, Dragovic, S, Spencer, C, Joyce, S, Killeen, N, Magnuson, MA, Boothby, M. Mammalian target of rapamycin protein complex 2 regulates differentiation of Th1 and Th2 cell subsets via distinct signaling pathways. Immunity, 32(6), 743-53, 2010

Boothby, M. The calculus of integrating differentiation: timing control of T-bet. Immunity, 30(5), 666-8, 2009

Cho, SH, Goenka, S, Henttinen, T, Gudapati, P, Reinikainen, A, Eischen, CM, Lahesmaa, R, Boothby, M. PARP-14, a member of the B aggressive lymphoma (BAL) family, transduces survival signals in primary B cells. Blood, 2009

Adeeku, E, Gudapati, P, Mendez-Fernandez, Y, Van Kaer, L, Boothby, M. Flexibility accompanies commitment of memory CD4 lymphocytes derived from IL-4 locus-activated precursors. Proc Natl Acad Sci U S A, 105(27), 9307-12, 2008

Goenka, S, Cho, SH, Boothby, M. Collaborator of Stat6 (CoaSt6)-associated poly(ADP-ribose) polymerase activity modulates Stat6-dependent gene transcription. J Biol Chem, 282(26), 18732-9, 2007

Talati, M, Meyrick, B, Peebles, RS, Davies, SS, Dworski, R, Mernaugh, R, Mitchell, D, Boothby, M, Roberts, LJ, Sheller, JR. Oxidant stress modulates murine allergic airway responses. Free Radic Biol Med, 40(7), 1210-9, 2006

Zhang, F, Boothby, M. T helper type 1-specific Brg1 recruitment and remodeling of nucleosomes positioned at the IFN-gamma promoter are Stat4 dependent. J Exp Med, 203(6), 1493-505, 2006

Goenka, S, Boothby, M. Selective potentiation of Stat-dependent gene expression by collaborator of Stat6 (CoaSt6), a transcriptional cofactor. Proc Natl Acad Sci U S A, 103(11), 4210-5, 2006

Corn, RA, Hunter, C, Liou, HC, Siebenlist, U, Boothby, MR. Opposing roles for RelB and Bcl-3 in regulation of T-box expressed in T cells, GATA-3, and Th effector differentiation. J Immunol, 175(4), 2102-10, 2005

Corn RA, Hunter C, Liou HC, Siebenlist U, Boothby M . Opposing roles for RelB and Bcl-3 in regulation of T-box expressed in T cells, GATA-3, and Th effector differentiation. J Immunol., 175(Aug 15 (4)), 2102-2110, 2005

Tong, Y, Aune, T, Boothby, M. T-bet antagonizes mSin3a recruitment and transactivates a fully methylated IFN-gamma promoter via a conserved T-box half-site. Proc Natl Acad Sci U S A, 102(6), 2034-9, 2005

Stephenson, LM, Park, DS, Mora, AL, Goenka, S, Boothby, M. Sequence motifs in IL-4R alpha mediating cell-cycle progression of primary lymphocytes. J Immunol, 175(8), 5178-85, 2005

Jones, RG, Saibil, SD, Pun, JM, Elford, AR, Bonnard, M, Pellegrini, M, Arya, S, Parsons, ME, Krawczyk, CM, Gerondakis, S, Yeh, WC, Woodgett, JR, Boothby, MR, Ohashi, PS. NF-kappaB couples protein kinase B/Akt signaling to distinct survival pathways and the regulation of lymphocyte homeostasis in vivo. J Immunol, 175(6), 3790-9, 2005

Aronica, MA, Swaidani, S, Zhang, YH, Mitchell, D, Mora, AL, McCarthy, S, O''Neal, J, Topham, D, Sheller, JR, Boothby, M. Susceptibility to allergic lung disease regulated by recall responses of dual-receptor memory T cells. J Allergy Clin Immunol, 114(6), 1441-8, 2004

Stanic, AK, Bezbradica, JS, Park, JJ, Van Kaer, L, Boothby, MR, Joyce, S. Cutting edge: the ontogeny and function of Va14Ja18 natural T lymphocytes require signal processing by protein kinase C theta and NF-kappa B. J Immunol, 172(8), 4667-71, 2004

Stephenson, L, Johns, MH, Woodward, E, Mora, AL, Boothby, M. An IL-4R alpha allelic variant, I50, acts as a gain-of-function variant relative to V50 for Stat6, but not Th2 differentiation. J Immunol, 173(7), 4523-8, 2004

Aronica MA, Swaidani, S, Zhang YH, Mitchell D, Mora AL, McCarthy S, Topham D, Sheller JR, and Boothby M. Susceptibility to allergic lung disease regulated by recall responses of dual-specificity memory T cells. J Allergy and Clin Immunol, in press, 2004

Stanic, Aleksandar K, Bezbradica, Jelena S, Park, Jang-June, Matsuki, Naoto, Mora, Ana L, Van Kaer, Luc, Boothby, Mark R, Joyce, Sebastian. NF-kappaB controls cell fate specification, survival, and molecular differentiation of immunoregulatory natural T lymphocytes. J Immunol, 172(4), 2265-73, 2004

Heller, NM, Matsukura, S, Georas, SN, Boothby, MR, Rothman, PB, Stellato, C, Schleimer, RP. Interferon-gamma inhibits STAT6 signal transduction and gene expression in human airway epithelial cells. Am J Respir Cell Mol Biol, 31(5), 573-82, 2004

Heller, NM, Matsukura, S, Georas, SN, Boothby, MR, Stellato, C, Schleimer, RP. Assessment of signal transducer and activator of transcription 6 as a target of glucocorticoid action in human airway epithelial cells. Clin Exp Allergy, 34(11), 1690-700, 2004

Kelly-Welch, Ann E, Hanson, Erica M, Boothby, Mark R, Keegan, Achsah D . Interleukin-4 and interleukin-13 signaling connections maps. Science, 300, 1527-8, 2003

Zhou, Ping, Hwang, Kwang Woo, Palucki, David A, Guo, Zhong, Boothby, Mark, Newell, Kenneth A, Alegre, Maria-Luisa. Impaired NF-kappaB activation in T cells permits tolerance to primary heart allografts and to secondary donor skin grafts. Am J Transplant, 3, 139-47, 2003

Tato, Cristina M, Villarino, Alejandro, Caamano JH, Jorge H, Boothby, Mark, Hunter, Christopher A, . Inhibition of NF-kappa B activity in T and NK cells results in defective effector cell expansion and production of IFN-gamma required for resistance to Toxoplasma gondii.. J Immunol, 170, 3139-46, 2003

Goenka, Shreevrat, Marlar, Clinton, Schindler, Ulrike, Boothby, Mark. Differential roles of C-terminal activation motifs in the establishment of Stat6 transcriptional specificity. J Biol Chem, 2003

Aronica, Mark A, McCarthy, Susan, Swaidani, Shadi, Mitchell, Daphne, Goral, Mehmet, Sheller, James R, Boothby, Mark. Recall Th2 response: Th1-resistant allergic susceptibility without biasing uncommitted CD4 T cells. Am J Respir Crit Care Med, 2003

Mora, Ana L, Stephenson, Linda M, Enerson, Ben, Youn, Jeehee, Keegan, Achsah D, Boothby, Mark, . New programming of IL-4 receptor signal transduction in activated T cells: Stat6 induction and Th2 differentiation mediated by IL-4Ralpha lacking cytoplasmic tyrosines.. J Immunol, 171:, 1891-1900, 2003

Corn, Radiah A, Aronica, Mark A, Zhang, Fuping, Tong, Yingkai, Stanley, Sarah A, Kim, Se Ryoung Agnes, Stephenson, Linda, Enerson, Ben, McCarthy, Susan, Mora, Ana, Boothby, Mark . T cell-intrinsic requirement for NF-kappaB induction in postdifferentiation IFN-gamma production and clonal expansion in a Th1 response. J Immunol, 171, 1816-1824, 2003

Boothby, Mark. Signaling advances from immunogenetics to immunogenomics. Genome Biol, 4(12), 239, 2003

Yang, Li, Yamagata, Noboru, Yadav, Rajwardhan, Brandon, Suzanne, Courtney, Regina L, Morrow, Jason D, Shyr, Yu, Boothby, Mark, Joyce, Sebastian, Carbone, David P, Breyer, Richard M. Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor. J Clin Invest, 111(5), 727-35, 2003

Mora, A L, Corn, R A, Stanic, A K, Goenka, S, Aronica, M, Stanley, S, Ballard, D W, Joyce, S, Boothby, M. Antiapoptotic function of NF-kappaB in T lymphocytes is influenced by their differentiation status: roles of Fas, c-FLIP, and Bcl-xL. Cell Death Differ, 10(9), 1032-44, 2003

Harlin, Helena, Podack, Eckhard, Boothby, Mark, Alegre, Maria-Luisa. TCR-independent CD30 signaling selectively induces IL-13 production via a TNF receptor-associated factor/p38 mitogen-activated protein kinase-dependent mechanism. J Immunol, 169(5), 2451-9, 2002

Harlin, Helena, Hwang, Kwang Woo, Palucki, David A, Kim, Oliver, Thompson, Craig B, Boothby, Mark, Alegre, Maria-Luisa. CTLA-4 engagement regulates NF-kappaB activation in vivo. Eur J Immunol, 32(8), 2095-104, 2002

Haley, Kathleen J, Ciota, Alex, Contreras, Johanna P, Boothby, Mark R, Perkins, David L, Finn, Patricia W. Alterations in lung collectins in an adaptive allergic immune response. Am J Physiol Lung Cell Mol Physiol, 282(3), L573-84, 2002

Soutto, Mohammed, Zhang, Feng, Enerson, Ben, Tong, Yingkai, Boothby, Mark, Aune, Thomas M, . A minimal IFN-gamma promoter confers Th1 selective expression.. J Immunol, 169, 4205-12, 2002

Boothby M and Aronica MA. Transcription regulation, allergic responses, and asthma . Immunol Allergy Clin N. Amer , 22, 871-889, 2002

Chen, Ying, Rosloniec, Ed, Price, Jim, Boothby, Mark, Chen, Jin. Constitutive expression of BCL-X(L) in the T lineage attenuates collagen-induced arthritis in Bcl-X(L) transgenic mice. Arthritis Rheum, 46(2), 514-21, 2002

Finn, Patricia W, He, Hongzhen, Ma, Chunyan, Mueller, Thomas, Stone, James R, Liou, Hsiou-Chi, Boothby, Mark R, Perkins, David L. Molecular profiling of the role of the NF-kappaB family of transcription factors during alloimmunity. J Leukoc Biol, 72(5), 1054-62, 2002

, 2001.

Boothby, M. Specificity of sn50 for NF-kappa B. Nat Immunol, 2(6), 471-2, 2001

Mora, A L, Stanley, S, Armistead, W, Chan, A C, Boothby, M. Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel. J Immunol, 167(10), 5628-35, 2001

Finn, P W, Stone, J R, Boothby, M R, Perkins, D L. Inhibition of NF-kappaB-dependent T cell activation abrogates acute allograft rejection. J Immunol, 167(10), 5994-6001, 2001

Mora A, Youn J, Keegan A, and Boothby, M . NF-kappa B/Rel participation in the lymphokine-dependent proliferation of T lymphoid cells. J Immunol, 166, 2218-2227, 2001

Chen, Y, Rosloniec, E, Goral, M I, Boothby, M, Chen, J. Redirection of T cell effector function in vivo and enhanced collagen-induced arthritis mediated by an IL-2R beta/IL-4R alpha chimeric cytokine receptor transgene. J Immunol, 166(6), 4163-9, 2001

Boothby, M, Mora, A L, Aronica, M A, Youn, J, Sheller, J R, Goenka, S, Stephenson, L, . IL-4 signaling, gene transcription regulation, and the control of effector T cells. Immunol Res, 23, 179-191, 2001

Boothby M, Mora AL, Stephenson, LM . Lymphokine-dependent proliferation of T-lymphoid cells: regulated responsiveness and role in vivo. Crit Rev Immunol, 21, 487-522, 2001

Zamorano, J, Mora, A L, Boothby, M, Keegan, A D. NF-kappa B activation plays an important role in the IL-4-induced protection from apoptosis. Int Immunol, 13(12), 1479-87, 2001

Aronica MA, Goenka S, and Boothby, M . IL-4-dependent induction of BCL-2 and BCL-x(L) in activated T lymphocytes through a STAT6- and PI 3-kinase-independent pathway. Cytokine, 12, 578-587, 2000

Aune, T M, Mora, A L, Kim, S, Boothby, M, Lichtman, A H. Costimulation reverses the defect in IL-2 but not effector cytokine production by T cells with impaired IkappaBalpha degradation. J Immunol, 162(10), 5805-12, 1999

Goenka, S, Youn, J, Dzurek, L M, Schindler, U, Yu-Lee, L Y, Boothby, M. Paired Stat6 C-terminal transcription activation domains required both for inhibition of an IFN-responsive promoter and trans-activation. J Immunol, 163(9), 4663-72, 1999

Aronica, M A, Mora, A L, Mitchell, D B, Finn, P W, Johnson, J E, Sheller, J R, Boothby, M R. Preferential role for NF-kappa B/Rel signaling in the type 1 but not type 2 T cell-dependent immune response in vivo. J Immunol, 163(9), 5116-24, 1999

Powell, W C, Fingleton, B, Wilson, C L, Boothby, M, Matrisian, L M. The metalloproteinase matrilysin proteolytically generates active soluble Fas ligand and potentiates epithelial cell apoptosis. Curr Biol, 9(24), 1441-7, 1999

Mora, A L, Chen, D, Boothby, M, Rubin, D H. Lineage-specific differences among CD8+ T cells in their dependence of NF-kappa B/Rel signaling. Eur J Immunol, 29(9), 2968-80, 1999

Seetharaman, R, Mora, A L, Nabozny, G, Boothby, M, Chen, J. Essential role of T cell NF-kappa B activation in collagen-induced arthritis. J Immunol, 163(3), 1577-83, 1999

Lee, S Y, Kaufman, D R, Mora, A L, Santana, A, Boothby, M, Choi, Y. Stimulus-dependent synergism of the antiapoptotic tumor necrosis factor receptor-associated factor 2 (TRAF2) and nuclear factor kappaB pathways. J Exp Med, 188(7), 1381-4, 1998

Youn, J, Chen, J, Goenka, S, Aronica, M A, Mora, A L, Correa, V, Sheller, J R, Boothby, M. In vivo function of an interleukin 2 receptor beta chain (IL-2Rbeta)/IL-4Ralpha cytokine receptor chimera potentiates allergic airway disease. J Exp Med, 188(10), 1803-16, 1998

Zhang, F, Wang, D Z, Boothby, M, Penix, L, Flavell, R A, Aune, T M. Regulation of the activity of IFN-gamma promoter elements during Th cell differentiation. J Immunol, 161(11), 6105-12, 1998

Wang, D, Zamorano, J, Keegan, A D, Boothby, M. HMG-I(Y) phosphorylation status as a nuclear target regulated through insulin receptor substrate-1 and the I4R motif of the interleukin-4 receptor. J Biol Chem, 272(40), 25083-90, 1997

Boothby, M R, Mora, A L, Scherer, D C, Brockman, J A, Ballard, D W. Perturbation of the T lymphocyte lineage in transgenic mice expressing a constitutive repressor of nuclear factor (NF)-kappaB. J Exp Med, 185(11), 1897-907, 1997

Wang, D Z, Cherrington, A, Famakin-Mosuro, B, Boothby, M. Independent pathways for de-repression of the mouse Ig heavy chain germ-line epsilon promoter: an IL-4 NAF/NF-IL-4 site as a context-dependent negative element. Int Immunol, 8(7), 977-89, 1996

Kovalik, J P, Ansari, B, Boothby, M. Rapid commitment to negative selection of a thymocyte line independent of CD4 co-engagement. J Immunol, 157(12), 5290-9, 1996

Kim, J, Reeves, R, Rothman, P, Boothby, M. The non-histone chromosomal protein HMG-I(Y) contributes to repression of the immunoglobulin heavy chain germ-line epsilon RNA promoter. Eur J Immunol, 25(3), 798-808, 1995

Wang, D Z, Ray, P, Boothby, M. Interleukin 4-inducible phosphorylation of HMG-I(Y) is inhibited by rapamycin. J Biol Chem, 270(39), 22924-32, 1995

Whitley, M Z, Cheng, H L, Tomasi, T B, Boothby, M. Distinct IL-4 response mechanisms of the MHC gene A alpha in different mouse B cell lines. Mol Immunol, 30(9), 821-32, 1993

Casey, L S, Lichtman, A H, Boothby, M. IL-4 induces IL-2 receptor p75 beta-chain gene expression and IL-2-dependent proliferation in mouse T lymphocytes. J Immunol, 148(11), 3418-26, 1992

Li, S C, Rothman, P, Boothby, M, Ferrier, P, Glimcher, L, Alt, F W. Control of immunoglobulin heavy chain constant region gene expression. Adv Exp Med Biol, 292, 245-51, 1991

Whitley, M Z, Sisk, R, Ivashkiv, L B, Finn, P W, Glimcher, L H, Boothby, M R. Non-consensus DNA sequences function in a cell-type-specific enhancer of the mouse class II MHC gene A alpha. Int Immunol, 3(9), 877-88, 1991

Gravallese, E M, Darling, J M, Glimcher, L H, Boothby, M. Role of lipopolysaccharide and IL-4 in control of transcription of the class II A alpha gene. J Immunol, 147(7), 2377-83, 1991

Rothman, P, Li, S C, Gorham, B, Glimcher, L, Alt, F, Boothby, M. Identification of a conserved lipopolysaccharide-plus-interleukin-4-responsive element located at the promoter of germ line epsilon transcripts. Mol Cell Biol, 11(11), 5551-61, 1991

Finn, P W, Kara, C J, Van, T T, Douhan, J, Boothby, M R, Glimcher, L H. The presence of a DNA binding complex correlates with E beta class II MHC gene expression. EMBO J, 9(5), 1543-9, 1990

Liou, H C, Boothby, M R, Finn, P W, Davidon, R, Nabavi, N, Zeleznik-Le, N J, Ting, J P, Glimcher, L H. A new member of the leucine zipper class of proteins that binds to the HLA DR alpha promoter. Science, 247(4950), 1581-4, 1990

Boothby, M, Liou, H C, Finn, P W, Gravallese, E, Glimcher, L H. Control of transcription at the murine A alpha locus. Immunol Res, 9(2), 77-92, 1990

Gravallese, E M, Boothby, M R, Smas, C M, Glimcher, L H. A lipopolysaccharide-induced DNA-binding protein for a class II gene in B cells is distinct from NF-kappa B. Mol Cell Biol, 9(8), 3184-92, 1989

Boothby, M, Liou, H C, Glimcher, L H. Differences in DNA sequence specificity among MHC class II X box binding proteins. J Immunol, 142(3), 1005-14, 1989

Boothby, M, Gravallese, E, Liou, H C, Glimcher, L H. A DNA binding protein regulated by IL-4 and by differentiation in B cells. Science, 242(4885), 1559-62, 1988

Liou, H C, Boothby, M R, Glimcher, L H. Distinct cloned class II MHC DNA binding proteins recognize the X box transcription element. Science, 242(4875), 69-71, 1988

Boime, I, Boothby, M, Darnell, R B, Policastro, P. Structure and expression of human placental hormone genes. Adv Exp Med Biol, 205, 267-80, 1986

Hoshina, M, Boothby, M, Hussa, R, Pattillo, R, Camel, H M, Boime, I. Linkage of human chorionic gonadotrophin and placental lactogen biosynthesis to trophoblast differentiation and tumorigenesis. Placenta, 6(2), 163-72, 1985

Hoshina, M, Boothby, M R, Hussa, R D, Pattillo, R A, Camel, H M, Boime, I. Segregation patterns of polymorphic restriction sites of the gene encoding the alpha subunit of human chorionic gonadotropin in trophoblastic disease. Proc Natl Acad Sci U S A, 81(8), 2504-7, 1984

Boothby, M, Kukowska, J, Boime, I. Imbalanced synthesis of human choriogonadotropin alpha and beta subunits reflects the steady state levels of the corresponding mRNAs. J Biol Chem, 258(15), 9250-3, 1983

Policastro, P, Ovitt, C E, Hoshina, M, Fukuoka, H, Boothby, M R, Boime, I. The beta subunit of human chorionic gonadotropin is encoded by multiple genes. J Biol Chem, 258(19), 11492-9, 1983

Hoshina, M, Boothby, M, Boime, I. Cytological localization of chorionic gonadotropin alpha and placental lactogen mRNAs during development of the human placenta. J Cell Biol, 93(1), 190-8, 1982

Boime, I, Boothby, M, Hoshina, M, Daniels-McQueen, S, Darnell, R. Expression and structures of human placental hormone genes as a function of placental development. Biol Reprod, 26(1), 73-91, 1982

Kohler, PO, Riser, M, Hardin, J, Boothby, M, Boime, I, Norris, J, Siciliano, MJ. Chorionic gonadotropin synthesis and gene assignment in human:mouse hybrid cells. Adv Exp Med Biol, 138, 405-18, 1981

Boothby, M, Ruddon, R W, Anderson, C, McWilliams, D, Boime, I. A single gonadotropin alpha-subunit gene in normal tissue and tumor-derived cell lines. J Biol Chem, 256(10), 5121-7, 1981