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Vanderbilt Pathology Laboratory Services

Test Directory



CHLAMYDIA TRACHOMATIS AMPLIFIED DNA ASSAY (CTD)

Department: Molecular Infectious Disease
Test Synonym(s): CTD, Chlamydia PCR
CPT Codes: CT-87491
Methodology: PCR- Polymerase Chain Reaction
Reference Range: Not Detected
Tube Type: Orange top M2000 Collection Swabs, Urine: 10 mL first voided
Specimen: Urine specimens (male or female), endocervical swabs, male urethral swabs, rectal swabs and oropharyngeal swabs
Alternate Specimen: Eye swab - sent to a reference lab: Click here for test information. Other specimens need Medical Director approval. Please call 615-875-5227 to speak with the lab.
Pediatric Requirements: Assay not approved for pediatric testing (contact lab)
Volume: 10.0 mL
Minimum Volume: 4.0 mL
Temperature: Urine - refrigerated, Swabs - room temperature if same day, refrigerated if overnight or up to 6 days after collection.
Stability: Refrigerated: 6 days; Frozen: 3 months (preferably -80C)
Reasons for Rejection: Specimen collected incorrectly; alternative specimen sent without Medical Director approval
Days Performed: Monday - Friday
Times Performed: Once per day
TAT: 1 - 3 days
Significance: Chlamydia trachomatis (CT) is the most common communicable notifiable disease in the United States. CT causes cervicitis, urethritis, salpingitis, proctitis, and endometritis in women and urethritis, epididymitis, and proctitis in men. This organism can also be transmitted via the birth canal, potentially resulting in infant conjunctivitis and/or chlamydial pneumonia in newborns. Current methods for detection of CT include culture, immunoassays, non-amplified probes, and nucleic acid amplification tests (NAATs). NAATs have demonstrated two advantages over non-amplified methods: increased sensitivity and applicability to a variety of sample types, including urine. NAATs have been accepted as the preferred method for laboratory diagnosis of CT infections. In addition to testing for genital CT infections, recent CDC recommendations reflect the clinical utility of NAA-based CT testing of rectal and oropharyngeal specimens collected from patients at increased risk of infection at these extragenital sites, in particular, men who have sex with men.
Special Instructions: Click here to print the Molecular Diagnostics requisition.

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