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Vanderbilt Pathology Laboratory Services

Test Directory



NEISSERIA GONORRHOEAE AMPLIFIED DNA ASSAY (GCD)

Department: Molecular Infectious Disease
Test Synonym(s): GCD, Gonorrhoea PCR, GC PCR
CPT Codes: GC-87591
Methodology: PCR - Polymerase Chain Reaction
Reference Range: Not detected
Tube Type: Orange top M2000 Collection swabs, Urine: 10 mL first voided
Specimen: Urine specimens (male or female), endocervical swabs, male urethral swabs, rectal swabs and oropharyngeal swabs
Alternate Specimen: Eye swab - sent to a reference lab: Click here for test information. Other specimens need Medical Director approval. Please call 615-875-5227 to speak with the lab.
Pediatric Requirements: Assay not approved for pediatric testing (contact lab)
Volume: 10.0 mL
Minimum Volume: 4.0 mL
Temperature: Urine - refrigerated, Swabs - room temperature if same day, refrigerated if overnight or up to 6 days after collection.
Stability: Refrigerated: 6 days; Frozen (preferably -80C): 3 months
Reasons for Rejection: Specimen collected incorrectly
Days Performed: Monday - Friday
Times Performed: Once per day
TAT: 1 - 3 days
Significance: "Neisseria gonorrhoeae (NG) infections are the second most common communicable notifiable diseases in the United States. NG causes acute urethritis in males, which if untreated can progress to epididymitis, prostatitis, and urethral stricture. In females, the primary site of infection is the endocervix. An important complication in females is development of pelvic inflammatory disease, which contributes to infertility. Asymptomatic infections occur often in females but infrequently in males. Current methods for detection of NG include culture, non-amplified probes, and nucleic acid amplification tests (NAATs). NAATs have demonstrated two advantages over non-amplified methods: increased sensitivity and applicability to a variety of sample types, including urine. NAATs have been accepted as the preferred method for laboratory diagnosis of NG infections. In addition to testing for genital NG infections, recent CDC recommendations reflect the clinical utility of NAA-based NG testing of rectal and oropharyngeal specimens collected from patients at increased risk of infection at these extragenital sites, in particular, men who have sex with men.
Special Instructions: Click here to print the Molecular Diagnostics requisition.

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