Vanderbilt Pathology Laboratory Services

VITAMIN D, 25 - OH, TOTAL (TVD)

Department: Esoteric Chemistry
Test Synonym(s): TVD, 25 - Hydroxy - Vitamin D, Total Vitamin D
CPT Codes: 82306
Methodology: Chemiluminescent Immunoassay
Reference Range: 25 - 80 ng/mL
Tube Type: Red top tube
Specimen: Serum
Alternate Specimen: None
Pediatric Requirements: 2 red microtainers
Volume: 2.0 mL
Minimum Volume: 0.5 mL
Temperature: Refrigerate
Stability: Separate within one hour and store at 2 - 8 C
Reasons for Rejection: Gross hemolysis or lipemia, improper collection, QNS, plasma sample received

Days Performed: Monday - Friday
Times Performed: 7:00 a.m. - 3:00 p.m.
TAT: 1 day
Significance: This test is appropriate for screening for 25-OH vitamin D status, diagnosis of vitamin D deficiency or toxicity, and/or monitoring of Vitamin D supplementation




<10 ng/mL (severe deficiency)




10-24 ng/mL (mild to moderate deficiency)




25-80 ng/mL (optimum levels)




>80 ng/mL (toxicity possible)

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