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Vanderbilt Pathology Laboratory Services

Test Directory



VITAMIN D, 25 - OH, TOTAL (TVD)

Department: Chemistry
Test Synonym(s): TVD, 25 - Hydroxy - Vitamin D, Total Vitamin D
CPT Codes: 82306
Methodology: Chemiluminescent Immunoassay
Reference Range: Vitamin D Status: Deficiency <10; Insufficiency 10-24; Sufficiency 25-80; Toxicity >80
Tube Type: Light green PST (Lithium Heparin) - 4.5 mL tube
Specimen: Plasma
Pediatric Requirements: 1 light green microtainer
Volume: 1 mL plasma
Minimum Volume: 0.4 mL plasma
Temperature: Refrigerate
Stability: Whole blood or separated plasma: Room temperature (15-30 C) - up to 3 days, Refrigerated (2-8 C) - up to 12 days, Frozen (-20 C) - 3 months. Avoid more than 4 freeze/thaw cycles
Reasons for Rejection: Gross hemolysis or lipemia, improper collection, QNS
Days Performed: Daily
Times Performed: Continuous
TAT: 1 day
Significance: This test is appropriate for screening for 25-OH vitamin D status, diagnosis of vitamin D deficiency or toxicity, and/or monitoring of Vitamin D supplementation
<10 ng/mL (severe deficiency)
10-24 ng/mL (mild to moderate deficiency)
25-80 ng/mL (optimum levels)
>80 ng/mL (toxicity possible)

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