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Vanderbilt Pathology Laboratory Services

Test Directory



VON WILLEBRAND PROFILE (VWP)

Department: Coagulation
Test Synonym(s): VWP, von WIllebrand workup, von Willebrand Mulitmer panel
Test Components: Fibrinogen, Factor VIII Activity, von Willebrand Antigen, Ristocetin Cofactor (von Willebrand activity), von Willebrand panel interpretation, von Willebrand Multimers if indicated
CPT Codes: 85384, 85240, 85246, 85245, 85390, 85247
Methodology: Clotting, Immunoturbidimetric, ristocetin induced agglutination of formalin fixed platelets, electrophoresis
Reference Range: Refer to individual test information
Tube Type: Two Light blue top (Sodium Citrate) - 2.7 mL
Specimen: Citrated platelet poor plasma - Requires double centrifugation if sending a plasma aliquot. Aliquot(s) must be frozen if not received by lab within two hours of collection.


Pediatric Requirements: Pediatric: Two 2.7mL Light blue top - 3.2% Sodium Citrate. Neonatal: Two 1.8mL Light blue top - 3.2% Sodium Citrate.


Volume: 2.0 Plasma
Minimum Volume: 2.0 Plasma
Temperature: Room Temperature for Light blue top tube, must be received within two hours of draw. Plasma aliquot(s) must be frozen if not received by lab within two hours of collection.


Stability: Room Temperature: 2 hours; Frozen: -70 C for one month
Reasons for Rejection: Clotted sample, incorrectly filled tubes, incorrect tube type, grossly hemolyzed sample, grossly lipemic sample sent as whole blood >2.0 hours old


Days Performed: Monday - Friday
TAT: 1-3 days, 7-14 days if the VWF Multimer is required.


Special Instructions: Deliver to lab immediately. Tubes must be filled to indicator line and not clotted. Indwelling lines should be flushed with saline only and 5 mL of blood should be discarded prior to collecting samples for coagulation studies. If sending plasma aliquots, DOUBLE CENTRIFUGE plasma samples to produce platelet poor plasma. Samples received from an outside lab must be aliquoted and frozen. Grossly hemlyzed, icteric or lipemic samples may be rejected. Ordering physician name and telephone contact information MUST accompany the sample and request. Vanderbilt Esoteric Coagulation: 615 875-5633



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