KIT D816V MUTATION (K17)
: Molecular Diagnostics
: K17, Kit Exon 17 (D816V), KIT D816V MUTATION, Kit 17
: Amplified by PCR using allele specific PCR for exon 17 of the KIT gene located on 4q12; evaluated by capillary electrophoresis; Laboratory Developed Test
: Lavender top (EDTA)
: Whole blood, bone marrow or purified DNA (1μg)
: Yellow top (ACDH) tube for whole blood or bone marrow
: 4 mL (whole blood, bone marrow)
: 0.5 mL (whole blood, bone marrow)
: EDTA, ACDH tubes: Refrigerated or room temperature; Purified DNA: Refrigerate or freeze
: EDTA and ACDH tubes: Room temperature - 2 days, Refrigerated - 3 days; Purified DNA: Indefinitely at -70 C
Reasons for Rejection
: Client will be notified by Molecular Diagnostics Lab if specimen is rejected
: 1 time per week, variable days
: 10 days
: Associated with Acute Myeloid Leukemia
: Call the Molecular Diagnostics Lab at 615-343-8121 or Molecular Diagnostics Lab Director at 615-343-9074 if additional assistance is needed regarding specimen requirements or submission. Please notify Molecular Diagnostics Lab if patient has received a transfusion in the prior 2 weeks.
Search Options for the Test Directory:
1. Enter the test name in the Keyword search field.
Hint: You can also enter any part of the test name to expand the search.
(Example: you can type "west" or "sed" or "rate" to find Westergren Sedimentation Rate)
2. Enter the Vanderbilt Test Code, if known.
3. Choose one of the letter boxes to display a list of all test names and keywords starting with that letter.
If you are unable to find a test in our directory, please contact VPLS at
1-800-551-5227 or e-mail firstname.lastname@example.org.