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Vanderbilt Pathology Laboratory Services

Test Directory



BRAIN LOH (1p, 10q, 17p AND 19q) (BLH)

Department: Molecular Diagnostics
Test Synonym(s): Loss of Heterozygosity, BLH
CPT Codes: 81479
Methodology: Detection of LOH associated with Gliomas at 1p, 10q, 17p and 19q by PCR and evaluated by capillary electrophoresis (tumor and normal samples)
Tube Type: Lavender top (EDTA)
Specimen: Control: Whole blood or normal paraffin embedded tissue and TUMOR - paraffin embedded tissue:block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10μm thick with accompanying before and after cut H&E slides

Pediatric Requirements: 0.5 mL (whole blood control)
Volume: 4 mL whole blood
Minimum Volume: 0.5 mL whole blood control
Temperature: EDTA : room temperature or refrigerated, Paraffin block: room temperature
Stability: EDTA - Room temperature: 2 days Refrigerated: 3 days; Paraffin block - indefinitely
Reasons for Rejection: Decalcification (paraffin embedded tissue. Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
Days Performed: Once a week - variable days
Times Performed: Batched
TAT: 7 - 14 days
Significance: Detection of LOH associated with Gliomas at 1p, 10q, 17p or 19q
Special Instructions: Referring clinician must specify which chromosomal sites analysis of LOH is requested. Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission.

Please notify Molecular Diagnostics Laboratory if patient has received a transfusion in the prior 2 weeks.
Click here to print the Molecular Diagnostics requisition.

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