.

Vanderbilt Pathology Laboratory Services

Test Directory



DNA ISOLATION (BANKING) (DNI)

Department: Molecular Diagnostics
Test Synonym(s): DNI
CPT Codes: 83891
Methodology: DNA Isolated and Banked for Future Studies
Tube Type: Lavender top (EDTA)
Specimen: Whole blood or bone marrow; 5-10mg Chorionic Villi; 2-3 T-25 flasks-cultured cells; paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10μm thick with accompanying before and after cut H&E slides); purified DNA (1μg)

Alternate Specimen: Yellow top (ACDH) for whole blood or bone marrow

Pediatric Requirements: 0.5 mL whole blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen type requirements

Volume: 4 mL (whole blood and bone marrow)
Minimum Volume: 0.5 mL whole blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen t
Temperature: EDTA : room temperature or refrigerated, Chorionic Villi: refrigerate, flasks (cultured cells): room temperature or refrigerate, purified DNA: refrigerate or freeze, paraffin block: room temperature

Stability: EDTA and ACDH tubes - Room temperature: 2 days Refrigerated: 3 days; Purified DNA - indefinitely @ -70C; Paraffin block - indefinitely; Chorionic villi and cultured cells - call Molecular Diagnostics Lab at 615-343-8121
Reasons for Rejection: Decalcification (paraffin embedded tissue) Client will be notified by Molecular Diagnostics Lab if specimen is rejected.

Days Performed: Monday - Friday
Times Performed: Batched
TAT: 5 - 7 days
Special Instructions: Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission. Please notify Molecular Dagnostics Laboratory if patient has received a transfusion in the prior 2 weeks. Chorionic Villi must be free of maternal cells; Cultured cells - backup culture must be maintained in event additional DNA is required.

Click here to print the Molecular Diagnostics requisition.

Search Tests

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Enter Keyword:

Search Help


Search Options for the Test Directory:

1. Enter the test name in the Keyword search field.
Hint: You can also enter any part of the test name to expand the search.
(Example: you can type "west" or "sed" or "rate" to find Westergren Sedimentation Rate)

2. Enter the Vanderbilt Test Code, if known.

3. Choose one of the letter boxes to display a list of all test names and keywords starting with that letter.

If you are unable to find a test in our directory, please contact VPLS at
1-800-551-5227 or e-mail emily.k.thompson@vanderbilt.edu.