DNA ISOLATION (BANKING) (DNI)
: Molecular Diagnostics
: DNA Isolated and Banked for Future Studies
: Lavender top (EDTA)
: Whole blood or bone marrow; 5-10mg Chorionic Villi; 2-3 T-25 flasks-cultured cells; paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10μm thick with accompanying before and after cut H&E slides); purified DNA (1μg)
: Yellow top (ACDH) for whole blood or bone marrow
: 0.5 mL whole blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen type requirements
: 4 mL (whole blood and bone marrow)
: 0.5 mL whole blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen t
: EDTA : room temperature or refrigerated, Chorionic Villi: refrigerate, flasks (cultured cells): room temperature or refrigerate, purified DNA: refrigerate or freeze, paraffin block: room temperature
: EDTA and ACDH tubes - Room temperature: 2 days Refrigerated: 3 days; Purified DNA - indefinitely @ -70°C; Paraffin block - indefinitely; Chorionic villi and cultured cells - call Molecular Diagnostics Lab at 615-343-8121
Reasons for Rejection
: Decalcification (paraffin embedded tissue) Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
: Monday - Friday
: 5 - 7 days
: Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission. Please notify Molecular Dagnostics Laboratory if patient has received a transfusion in the prior 2 weeks. Chorionic Villi must be free of maternal cells; Cultured cells - backup culture must be maintained in event additional DNA is required.Click here to print the Molecular Diagnostics requisition.
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