Vanderbilt Pathology Laboratory Services
Test Directory
FLT3 INTERNAL TANDEM DUPLICATION (FL3)
Department: Molecular DiagnosticsTest Synonym(s): FL3, FLT3-ITD
CPT Codes: 81245
Methodology: Amplified by PCR using primer pairs specific for exon 14 and 15 of the FLT3 gene; evaluated by capillary electrophoresis
Tube Type: Lavender top (EDTA)
Specimen: Whole blood, bone marrow or purified DNA (1ìg)
Alternate Specimen: Yellow top (ACDH) for whole blood or bone marrow
Pediatric Requirements: 0.5 mL whole blood or bone marrow
Volume: 4 mL (whole blood, bone marrow)
Minimum Volume: 0.5 mL whole blood or bone marrow
Temperature: EDTA, ACDH tubes: room temperature or refrigerated, purified DNA: refrigerate or freeze
Stability: EDTA and citrate: Room temperature: 2 days Refrigerated: 3 days; Purified DNA: indefinitely at -70°C
Reasons for Rejection: Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
Days Performed: Monday and Thursday
Times Performed: Batched
TAT: 7 - 10 days
Significance: Associated with Acute Myeloid Leukemia
Special Instructions: Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission. Please notify Molecular Genetics Laboratory if patient has received a transfusion in the prior 2 weeks.
Click here to print the Molecular Diagnostics requisition.
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