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Vanderbilt Pathology Laboratory Services

Test Directory



IGH GENE REARRANGEMENT BY PCR (IGR)

Department: Molecular Diagnostics
Test Synonym(s): B cell gene rearrangement, IGR
CPT Codes: 81261
Methodology: PCR and evaluated by gel electrophoresis
Tube Type: Lavender top (EDTA)
Specimen: Whole blood, bone marrow, paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10m thick with accompanying before and after cut H&E slides); purified DNA (1g), CSF
Alternate Specimen: Yellow top (ACDH) for whole blood or bone marrow
Pediatric Requirements: 0.5 mL whole blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen type requirements
Volume: 4 mL (whole blood, bone marrow)
Minimum Volume: 0.5 mL whole blood or bone marrow
Temperature: EDTA and ACDH tubes: room temperature or refrigerated, purified DNA: refrigerate or freeze, paraffin block: room temperature; CSF - refrigerate
Stability: EDTA and ACDH tubes - Room temperature: 2 days Refrigerated: 3 days; Purified DNA - indefinitely @ -70C; Paraffin block - indefinitely; CSF - call Molecular Diagnostics Lab at 615-343-8121
Reasons for Rejection: Decalcification (paraffin embedded tissue)Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
Days Performed: Tuesday
Times Performed: Batched
TAT: 7 - 14 days
Significance: Detection of a clonal population of cells by PCR
Special Instructions: Call the Molecular Diagnostics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission or if patient has received a transfusion in the prior 2 weeks.
Click here to print the Molecular Diagnostics requisition.

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