MICROSATELLITE INSTABILITY (MSI)
: Molecular Diagnostics
: Amplified by PCR using fluorescent primers specific for 5 mononucleotide loci; analyzed by capillary electrophoresis
: Lavender top (EDTA) for control
: Control: whole blood or normal tissue (fresh, frozen or paraffin) AND Tumor tissue (fresh, frozen or paraffin block)
: Light blue top (Sodium Citrate) for control
: 3-15 mL whole blood
: 0.5 mL whole blood
: EDTA and Citrate tubes: room temperature or refrigerated, paraffin block: room temperature, fresh tissue: freeze or may be refrigerated in transport media.
: EDTA and citrate: Room temperature: 2 days Refrigerated: 3 days
Reasons for Rejection
: Client will be notified by Molecular Genetics Lab if specimen is rejected.
: Once a week - variable days
: 10 days
: Used for Somatic Genetic Disease testing (associated with Colorectal Cancer)
: Call the Molecular Genetics Laboratory at 615-343-8121 if additional assistance is needed regarding specimen requirements or submission. Please notify Molecular Genetics Laboratory if patient has received a transfusion in the prior 2 weeks. Click here to print the Molecular Diagnostics requisition.
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